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Literature summary extracted from

  • Ohe, T.; Watanabe, Y.
    Purification and properties of hypoxanthine phosphoribosyltransferase from Streptomyces cyanogenus (1980), Agric. Biol. Chem., 44, 1999-2006.
No PubMed abstract available

General Stability

EC Number General Stability Organism
2.4.2.8 5-phospho-alpha-D-ribose 1-diphosphate stabilizes against heat inactivation Streptomyces cyanogenus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.8 ATP
-
 Streptomyces cyanogenus
2.4.2.8 EDTA
-
 Streptomyces cyanogenus
2.4.2.8 GDP
-
 Streptomyces cyanogenus
2.4.2.8 GMP
-
 Streptomyces cyanogenus
2.4.2.8 GTP
-
 Streptomyces cyanogenus
2.4.2.8 IDP
-
 Streptomyces cyanogenus
2.4.2.8 IMP
-
 Streptomyces cyanogenus
2.4.2.8 purine nucleotides
-
 Streptomyces cyanogenus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.8 0.0016
-
 hypoxanthine
-
 Streptomyces cyanogenus
2.4.2.8 0.0027
-
 guanine
-
 Streptomyces cyanogenus
2.4.2.8 0.063
-
 5-phospho-alpha-D-ribose 1-diphosphate
-
 Streptomyces cyanogenus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.2.8 Mg2+ activating effect of Mn2+ is higher than that of Mg2+ Streptomyces cyanogenus
2.4.2.8 Mn2+ activating effect of Mn2+ is higher than that of Mg2+ Streptomyces cyanogenus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.8 18000
-
 8 * 18000, SDS-PAGE Streptomyces cyanogenus
2.4.2.8 150000
-
 gel filtration Streptomyces cyanogenus

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.8 Streptomyces cyanogenus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.8
-
 Streptomyces cyanogenus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.8 66.5
-
 purified enzyme Streptomyces cyanogenus

Storage Stability

EC Number Storage Stability Organism
2.4.2.8 4°C, 50 mM Tris-HCl, pH 7.8, 1 M KCl, 10 mM MgCl2, stable at least 1 month Streptomyces cyanogenus
2.4.2.8 4°C, 50 mM Tris-HCl, pH 7.8, complete loss of activity after 4 days Streptomyces cyanogenus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.8 guanine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Streptomyces cyanogenus GMP + diphosphate
-
 ?
2.4.2.8 hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Streptomyces cyanogenus IMP + diphosphate
-
 ?
2.4.2.8 additional information no substrate: adenine Streptomyces cyanogenus ?
-
 ?
2.4.2.8 xanthine + 5-phospho-alpha-D-ribose 1-diphosphate poor substrate Streptomyces cyanogenus XMP + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.8 octamer 8 * 18000, SDS-PAGE Streptomyces cyanogenus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.2.8 30
-
 pH 7.4, stable Streptomyces cyanogenus
2.4.2.8 40
-
 pH 7.4, gradual decrease of activity Streptomyces cyanogenus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.8 additional information
-
 pI: 4.4 Streptomyces cyanogenus
2.4.2.8 8.5
-
-
 Streptomyces cyanogenus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.2.8 5.8 9.7
-
 Streptomyces cyanogenus
2.4.2.8 6.3 9.7 pH 6.3: about 50% of activity maximum, pH 9.7: about 75% of activity maximum Streptomyces cyanogenus