BRENDA - Enzyme Database

Protein O-mannosylation in Candida albicans. Determination of the amino acid sequences of peptide acceptors for protein O-mannosyltransferase

Weston, A.; Nassau, P.M.; Henley, C.; Marriott, M.S.; Eur. J. Biochem. 215, 845-849 (1993)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.4.1.109
phosphatidylcholine
stimulates
Candida albicans
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.1.109
Mg2+
above 5 mM
Candida albicans
2.4.1.109
phosphatidylinositol
-
Candida albicans
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.1.109
0.075
-
biotin-Tyr-Thr-Ala-Val-NH2
-
Candida albicans
2.4.1.109
0.1
-
biotin-Tyr-Leu-Ala-Val-NH2
-
Candida albicans
2.4.1.109
0.25
-
Ac-Tyr-Ala-Thr-Ala-Val-NH2
-
Candida albicans
2.4.1.109
0.85
-
biotin-Tyr-Pro-Thr-Ala-Val-NH2
-
Candida albicans
2.4.1.109
2.2
-
Tyr-Ala-Thr-Ala-Val
-
Candida albicans
2.4.1.109
2.5
-
Tyr-Leu-Thr-Ala-Val
-
Candida albicans
2.4.1.109
4.3
-
Ac-Tyr-Asn-Pro-Thr-Ser-Val-NH2
-
Candida albicans
2.4.1.109
6.7
-
Ac-Ala-Thr-Ala-NH2
-
Candida albicans
2.4.1.109
7.3
-
Tyr-Pro-Thr-Ala-Val
-
Candida albicans
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.1.109
membrane
-
Candida albicans
16020
-
2.4.1.109
microsome
-
Candida albicans
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.1.109
Mg2+
not required
Candida albicans
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.4.1.109
dolichyl phosphate D-mannose + protein
Candida albicans
production of cell-wall mannoproteins
dolichyl phosphate + O-D-mannosylprotein
-
-
-
2.4.1.109
dolichyl phosphate D-mannose + protein
Candida albicans 2005 E
production of cell-wall mannoproteins
dolichyl phosphate + O-D-mannosylprotein
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.1.109
Candida albicans
-
2005 E
-
2.4.1.109
Candida albicans 2005 E
-
2005 E
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.4.1.109
0.0000012
-
substrate YNPTSV
Candida albicans
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.109
dolichyl phosphate D-mannose + Ac-Ala-Thr-Ala-NH2
-
638330
Candida albicans
dolichyl phosphate + O-D-mannosyl-Ac-Ala-Thr-Ala-NH2
-
638330
Candida albicans
?
2.4.1.109
dolichyl phosphate D-mannose + Ac-Tyr-Ala-Thr-Ala-Val-NH2
-
638330
Candida albicans
dolichyl phosphate + O-D-mannosyl-Ac-Tyr-Ala-Thr-Ala-Val-NH2
-
638330
Candida albicans
?
2.4.1.109
dolichyl phosphate D-mannose + Ac-Tyr-Asn-Pro-Thr-Ser-Val-NH2
-
638330
Candida albicans
dolichyl phosphate + O-D-mannosyl-Ac-Tyr-Asn-Pro-Thr-Ser-Val-NH2
-
638330
Candida albicans
?
2.4.1.109
dolichyl phosphate D-mannose + Ac-Tyr-Asn-Pro-Thr-Ser-Val-NH2
-
638330
Candida albicans 2005 E
dolichyl phosphate + O-D-mannosyl-Ac-Tyr-Asn-Pro-Thr-Ser-Val-NH2
-
638330
Candida albicans 2005 E
?
2.4.1.109
dolichyl phosphate D-mannose + biotin-Tyr-Ala-Thr-Ala-Val-NH2
-
638330
Candida albicans
dolichyl phosphate + O-D-mannosyl-N-biotinyl-Tyr-Ala-Thr-Ala-Val-NH2
-
638330
Candida albicans
?
2.4.1.109
dolichyl phosphate D-mannose + biotin-Tyr-Pro-Thr-Ala-Val-NH2
-
638330
Candida albicans
dolichyl phosphate + O-D-mannosyl-N-biotinyl-Tyr-Pro-Thr-Ala-Val-NH2
-
638330
Candida albicans
?
2.4.1.109
dolichyl phosphate D-mannose + biotin-Tyr-Thr-Ala-Val-NH2
-
638330
Candida albicans
dolichyl phosphate + O-D-mannosyl-N-biotinyl-Tyr-Thr-Ala-Val-NH2
-
638330
Candida albicans
?
2.4.1.109
dolichyl phosphate D-mannose + protein
-
638330
Candida albicans
dolichyl phosphate + O-D-mannosylprotein
-
638330
Candida albicans
?
2.4.1.109
dolichyl phosphate D-mannose + protein
the enzyme transfers mannosyl residues to the hydroxyl of serine or threonine residues
638330
Candida albicans
dolichyl phosphate + O-D-mannosylprotein
-
638330
Candida albicans
?
2.4.1.109
dolichyl phosphate D-mannose + protein
production of cell-wall mannoproteins
638330
Candida albicans
dolichyl phosphate + O-D-mannosylprotein
-
-
-
-
2.4.1.109
dolichyl phosphate D-mannose + protein
-
638330
Candida albicans 2005 E
dolichyl phosphate + O-D-mannosylprotein
-
638330
Candida albicans 2005 E
?
2.4.1.109
dolichyl phosphate D-mannose + protein
the enzyme transfers mannosyl residues to the hydroxyl of serine or threonine residues
638330
Candida albicans 2005 E
dolichyl phosphate + O-D-mannosylprotein
-
638330
Candida albicans 2005 E
?
2.4.1.109
dolichyl phosphate D-mannose + protein
production of cell-wall mannoproteins
638330
Candida albicans 2005 E
dolichyl phosphate + O-D-mannosylprotein
-
-
-
-
2.4.1.109
dolichyl phosphate D-mannose + Tyr-Ala-Thr-Ala-Val
-
638330
Candida albicans
dolichyl phosphate + O-D-mannosyl-Tyr-Ala-Thr-Ala-Val
-
638330
Candida albicans
?
2.4.1.109
dolichyl phosphate D-mannose + Tyr-Asn-Pro-Thr-Ser-Val
-
638330
Candida albicans
dolichyl phosphate + O-D-mannosyl-Tyr-Asn-Pro-Thr-Ser-Val
-
638330
Candida albicans
?
2.4.1.109
dolichyl phosphate D-mannose + Tyr-Asn-Pro-Thr-Ser-Val
-
638330
Candida albicans 2005 E
dolichyl phosphate + O-D-mannosyl-Tyr-Asn-Pro-Thr-Ser-Val
-
638330
Candida albicans 2005 E
?
2.4.1.109
dolichyl phosphate D-mannose + Tyr-Leu-Thr-Ala-Val
-
638330
Candida albicans
dolichyl phosphate + O-D-mannosyl-Tyr-Leu-Thr-Ala-Val
-
638330
Candida albicans
?
2.4.1.109
dolichyl phosphate D-mannose + Tyr-Pro-Thr-Ala-Val
-
638330
Candida albicans
dolichyl phosphate + O-D-mannosyl-Tyr-Pro-Thr-Ala-Val
-
638330
Candida albicans
?
Temperature Optimum [░C]
EC Number
Temperature Optimum [░C]
Temperature Optimum Maximum [░C]
Commentary
Organism
2.4.1.109
25
-
assay at
Candida albicans
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.1.109
7.5
8
the best buffers are bicine pH 7.7, tricine pH 8.0 and HEPES pH 7.5
Candida albicans
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.4.1.109
phosphatidylcholine
stimulates
Candida albicans
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.1.109
Mg2+
above 5 mM
Candida albicans
2.4.1.109
phosphatidylinositol
-
Candida albicans
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.1.109
0.075
-
biotin-Tyr-Thr-Ala-Val-NH2
-
Candida albicans
2.4.1.109
0.1
-
biotin-Tyr-Leu-Ala-Val-NH2
-
Candida albicans
2.4.1.109
0.25
-
Ac-Tyr-Ala-Thr-Ala-Val-NH2
-
Candida albicans
2.4.1.109
0.85
-
biotin-Tyr-Pro-Thr-Ala-Val-NH2
-
Candida albicans
2.4.1.109
2.2
-
Tyr-Ala-Thr-Ala-Val
-
Candida albicans
2.4.1.109
2.5
-
Tyr-Leu-Thr-Ala-Val
-
Candida albicans
2.4.1.109
4.3
-
Ac-Tyr-Asn-Pro-Thr-Ser-Val-NH2
-
Candida albicans
2.4.1.109
6.7
-
Ac-Ala-Thr-Ala-NH2
-
Candida albicans
2.4.1.109
7.3
-
Tyr-Pro-Thr-Ala-Val
-
Candida albicans
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.1.109
membrane
-
Candida albicans
16020
-
2.4.1.109
microsome
-
Candida albicans
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.1.109
Mg2+
not required
Candida albicans
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.4.1.109
dolichyl phosphate D-mannose + protein
Candida albicans
production of cell-wall mannoproteins
dolichyl phosphate + O-D-mannosylprotein
-
-
-
2.4.1.109
dolichyl phosphate D-mannose + protein
Candida albicans 2005 E
production of cell-wall mannoproteins
dolichyl phosphate + O-D-mannosylprotein
-
-
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.4.1.109
0.0000012
-
substrate YNPTSV
Candida albicans
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.109
dolichyl phosphate D-mannose + Ac-Ala-Thr-Ala-NH2
-
638330
Candida albicans
dolichyl phosphate + O-D-mannosyl-Ac-Ala-Thr-Ala-NH2
-
638330
Candida albicans
?
2.4.1.109
dolichyl phosphate D-mannose + Ac-Tyr-Ala-Thr-Ala-Val-NH2
-
638330
Candida albicans
dolichyl phosphate + O-D-mannosyl-Ac-Tyr-Ala-Thr-Ala-Val-NH2
-
638330
Candida albicans
?
2.4.1.109
dolichyl phosphate D-mannose + Ac-Tyr-Asn-Pro-Thr-Ser-Val-NH2
-
638330
Candida albicans
dolichyl phosphate + O-D-mannosyl-Ac-Tyr-Asn-Pro-Thr-Ser-Val-NH2
-
638330
Candida albicans
?
2.4.1.109
dolichyl phosphate D-mannose + Ac-Tyr-Asn-Pro-Thr-Ser-Val-NH2
-
638330
Candida albicans 2005 E
dolichyl phosphate + O-D-mannosyl-Ac-Tyr-Asn-Pro-Thr-Ser-Val-NH2
-
638330
Candida albicans 2005 E
?
2.4.1.109
dolichyl phosphate D-mannose + biotin-Tyr-Ala-Thr-Ala-Val-NH2
-
638330
Candida albicans
dolichyl phosphate + O-D-mannosyl-N-biotinyl-Tyr-Ala-Thr-Ala-Val-NH2
-
638330
Candida albicans
?
2.4.1.109
dolichyl phosphate D-mannose + biotin-Tyr-Pro-Thr-Ala-Val-NH2
-
638330
Candida albicans
dolichyl phosphate + O-D-mannosyl-N-biotinyl-Tyr-Pro-Thr-Ala-Val-NH2
-
638330
Candida albicans
?
2.4.1.109
dolichyl phosphate D-mannose + biotin-Tyr-Thr-Ala-Val-NH2
-
638330
Candida albicans
dolichyl phosphate + O-D-mannosyl-N-biotinyl-Tyr-Thr-Ala-Val-NH2
-
638330
Candida albicans
?
2.4.1.109
dolichyl phosphate D-mannose + protein
-
638330
Candida albicans
dolichyl phosphate + O-D-mannosylprotein
-
638330
Candida albicans
?
2.4.1.109
dolichyl phosphate D-mannose + protein
the enzyme transfers mannosyl residues to the hydroxyl of serine or threonine residues
638330
Candida albicans
dolichyl phosphate + O-D-mannosylprotein
-
638330
Candida albicans
?
2.4.1.109
dolichyl phosphate D-mannose + protein
production of cell-wall mannoproteins
638330
Candida albicans
dolichyl phosphate + O-D-mannosylprotein
-
-
-
-
2.4.1.109
dolichyl phosphate D-mannose + protein
-
638330
Candida albicans 2005 E
dolichyl phosphate + O-D-mannosylprotein
-
638330
Candida albicans 2005 E
?
2.4.1.109
dolichyl phosphate D-mannose + protein
the enzyme transfers mannosyl residues to the hydroxyl of serine or threonine residues
638330
Candida albicans 2005 E
dolichyl phosphate + O-D-mannosylprotein
-
638330
Candida albicans 2005 E
?
2.4.1.109
dolichyl phosphate D-mannose + protein
production of cell-wall mannoproteins
638330
Candida albicans 2005 E
dolichyl phosphate + O-D-mannosylprotein
-
-
-
-
2.4.1.109
dolichyl phosphate D-mannose + Tyr-Ala-Thr-Ala-Val
-
638330
Candida albicans
dolichyl phosphate + O-D-mannosyl-Tyr-Ala-Thr-Ala-Val
-
638330
Candida albicans
?
2.4.1.109
dolichyl phosphate D-mannose + Tyr-Asn-Pro-Thr-Ser-Val
-
638330
Candida albicans
dolichyl phosphate + O-D-mannosyl-Tyr-Asn-Pro-Thr-Ser-Val
-
638330
Candida albicans
?
2.4.1.109
dolichyl phosphate D-mannose + Tyr-Asn-Pro-Thr-Ser-Val
-
638330
Candida albicans 2005 E
dolichyl phosphate + O-D-mannosyl-Tyr-Asn-Pro-Thr-Ser-Val
-
638330
Candida albicans 2005 E
?
2.4.1.109
dolichyl phosphate D-mannose + Tyr-Leu-Thr-Ala-Val
-
638330
Candida albicans
dolichyl phosphate + O-D-mannosyl-Tyr-Leu-Thr-Ala-Val
-
638330
Candida albicans
?
2.4.1.109
dolichyl phosphate D-mannose + Tyr-Pro-Thr-Ala-Val
-
638330
Candida albicans
dolichyl phosphate + O-D-mannosyl-Tyr-Pro-Thr-Ala-Val
-
638330
Candida albicans
?
Temperature Optimum [░C] (protein specific)
EC Number
Temperature Optimum [░C]
Temperature Optimum Maximum [░C]
Commentary
Organism
2.4.1.109
25
-
assay at
Candida albicans
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.1.109
7.5
8
the best buffers are bicine pH 7.7, tricine pH 8.0 and HEPES pH 7.5
Candida albicans