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Literature summary extracted from

  • Peterbauer, T.; Mach, L.; Mucha, J.; Richter, A.
    Functional expression of a cDNA encoding pea (Pisum sativum L.) raffinose synthase, partial purification of the enzyme from maturing seeds, and steady-state kinetic analysis of raffinose synthesis (2002), Planta, 215, 839-846.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.82 functional expression as His-tagged protein in Spodoptera frugiperda Sf21 cells via baculovirus infection Pisum sativum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.82 1-deoxygalactonojirimycin strong, competitive towards galactinol Pisum sativum
2.4.1.82 myo-inositol competitive against sucrose Pisum sativum
2.4.1.82 Ni2+ destabilization, 30.2% activity at 1 mM Pisum sativum
2.4.1.82 sucrose
-
 Pisum sativum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.82 1
-
 galactinol hydrolysis Pisum sativum
2.4.1.82 7.3
-
 galactinol partially purified native enzyme, raffinose synthesis Pisum sativum
2.4.1.82 7.9
-
 galactinol recombinant enzyme in Sf21 cell extract, raffinose synthesis Pisum sativum
2.4.1.82 22.6
-
 sucrose recombinant enzyme in Sf21 cell extract, raffinose synthesis Pisum sativum
2.4.1.82 22.9
-
 sucrose partially purified native enzyme, raffinose synthesis Pisum sativum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.82 88700
-
 amino acid sequence determination Pisum sativum

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.82 Pisum sativum Q8VWN6 cv. Wunder von Kelvedon
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.82 no success in purifying the recombinant His-tagged enzyme from Sf21 insect cells Pisum sativum
2.4.1.82 partial Pisum sativum

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.1.82 alpha-D-galactosyl-(1->3)-1D-myo-inositol + sucrose = myo-inositol + raffinose ping-pong reaction mechanism Pisum sativum
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.82 seed maturing Pisum sativum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.82 0.00005
-
 recombinant in Sf21 insect cells Pisum sativum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.82 1-alpha-D-galactosyl-myo-inositol + D-ononitol D-ononitol is 1-D-4-O-methyl-myo-inositol Pisum sativum myo-inositol + alpha-D-galactosyl-D-ononitol
-
 r
2.4.1.82 1-alpha-D-galactosyl-myo-inositol + D-pinitol D-pinitol is 1-D-3-O-methyl-chiro-inositol Pisum sativum myo-inositol + galactopinitol A i.e. O-alpha-D-galactopyranosyl-(1,2)-4-O-methyl-D-chiro-inositol r
2.4.1.82 1-alpha-D-galactosyl-myo-inositol + H2O hydrolysis, in the absence of acceptor substrate Pisum sativum myo-inositol + D-galactose
-
 ?
2.4.1.82 1-alpha-D-galactosyl-myo-inositol + sucrose
-
 Pisum sativum myo-inositol + raffinose
-
 r
2.4.1.82 4-nitrophenol-alpha-D-galactopyranoside + sucrose
-
 Pisum sativum 4-nitrophenol + raffinose
-
 ?
2.4.1.82 alpha-D-galactosyl-D-ononitol + sucrose
-
 Pisum sativum D-ononitol + raffinose
-
 ?
2.4.1.82 additional information substrate specificity Pisum sativum ?
-
 ?
2.4.1.82 additional information raffinose is no acceptor substrate Pisum sativum ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.82 raffinose synthase
-
 Pisum sativum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.82 30
-
 assay at Pisum sativum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.82 7
-
-
 Pisum sativum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.1.82 0.189
-
 1-deoxygalactonojirimycin
-
 Pisum sativum
2.4.1.82 3.7
-
 sucrose hydrolysis reaction of galactinol, noncompetitive against galactinol Pisum sativum
2.4.1.82 10.1
-
 myo-inositol competitive against sucrose Pisum sativum
2.4.1.82 22.3
-
 myo-inositol noncompetitive against galactinol Pisum sativum