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Literature summary extracted from

  • Brockhausen, I.; Hull, E.; Hindsgaul, O.; Schachter, H.; Shah, R.N.; Michnick, S.W.; Carver, J.P.
    Control of glycoprotein synthesis. Detection and characterization of a novel branching enzyme from hen oviduct, UDP-N-acetylglucosamine:GlcNAc beta 1-6 (GlcNAc beta 1-2)Man alpha-R (GlcNAc to Man) beta-4-N-acetylglucosaminyltransferase VI (1989), J. Biol. Chem., 264, 11211-11221.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.201 Triton X-100 activation, 0.125-1.25% Gallus gallus
2.4.1.201 Triton X-100 activation, 0.125-1.25% Meleagris gallopavo
2.4.1.201 Triton X-100 activation, 0.125-1.25% Anas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.201 AMP 0.0125 mM, in the presence of 0.125 M N-acetylglucosamine Gallus gallus
2.4.1.201 ATP 0.0125 mM, in the presence of 0.125 M N-acetylglucosamine Gallus gallus
2.4.1.201 EDTA
-
 Gallus gallus
2.4.1.201 N-acetylglucosamine weak, 0.125 M Gallus gallus
2.4.1.201 UDP 0.0125 mM, in the presence of 0.125 M N-acetylglucosamine Gallus gallus
2.4.1.201 UDP-hexanolamine 0.0125 mM, in the presence of 0.125 M N-acetylglucosamine Gallus gallus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.201 0.09
-
 N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2)-beta-D-mannosyl-R
-
 Gallus gallus
2.4.1.201 0.6
-
 UDP-N-acetyl-D-glucosamine
-
 Gallus gallus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.201 microsome
-
 Gallus gallus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.201 Ca2+ activation, 12.5 mM, can substitute partially for Mn2+ Gallus gallus
2.4.1.201 Co2+ activation, 12.5 mM Gallus gallus
2.4.1.201 Co2+ can substitute partially for Mn2+ Gallus gallus
2.4.1.201 Mg2+ activation, 12.5 mM Gallus gallus
2.4.1.201 Mg2+ can substitute partially for Mn2+ Gallus gallus
2.4.1.201 Mn2+ absolute requirement Gallus gallus
2.4.1.201 Mn2+ absolute requirement Meleagris gallopavo
2.4.1.201 Mn2+ absolute requirement Anas sp.
2.4.1.201 Mn2+ maximal activity at 0.05-0.1 M Gallus gallus
2.4.1.201 Mn2+ maximal activity at 0.05-0.1 M Meleagris gallopavo
2.4.1.201 Mn2+ maximal activity at 0.05-0.1 M Anas sp.
2.4.1.201 additional information no activation by 12.5 mM Cd2+, Ni2+ or Zn2+ Gallus gallus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.201 UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2)-beta-D-mannosyl-R Meleagris gallopavo
-
 UDP + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2-beta)-(N-acetyl-D-glucosaminyl-1,4)-beta-D-mannosyl-R
-
 ?
2.4.1.201 UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2)-beta-D-mannosyl-R Anas sp.
-
 UDP + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2-beta)-(N-acetyl-D-glucosaminyl-1,4)-beta-D-mannosyl-R
-
 ?
2.4.1.201 UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2)-beta-D-mannosyl-R Gallus gallus reaction in biosynthesis of penta-antennary oligosaccharides of hen ovomucoid UDP + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2-beta)-(N-acetyl-D-glucosaminyl-1,4)-beta-D-mannosyl-R
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.201 Anas sp.
-
 duck
-
2.4.1.201 Gallus gallus
-
-
-
2.4.1.201 Meleagris gallopavo
-
 turkey
-
2.4.1.201 no activity in Bos taurus
-
 kidney
-
2.4.1.201 no activity in Homo sapiens
-
 colon
-
2.4.1.201 no activity in Rattus norvegicus
-
 colon and liver
-
2.4.1.201 no activity in Sus scrofa
-
 colon and stomach
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.201 colon
-
 Gallus gallus
-
2.4.1.201 colon
-
 Anas sp.
-
2.4.1.201 intestine
-
 Meleagris gallopavo
-
2.4.1.201 liver
-
 Gallus gallus
-
2.4.1.201 additional information tissue distribution Gallus gallus
-
2.4.1.201 oviduct
-
 Gallus gallus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.201 UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2)-alpha-mannosyl-1,6-beta-D-mannosyl-(CH2)8-CH3 synthetic acceptor substrate Gallus gallus UDP + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2-beta)-(N-acetyl-D-glucosaminyl-1,4)-beta-D-mannosyl-(CH2)8-CH3
-
 ?
2.4.1.201 UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2)-alpha-mannosyl-1,6-beta-D-mannosyl-(CH2)8-COOH synthetic acceptor substrate Gallus gallus UDP + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2-beta)-(N-acetyl-D-glucosaminyl-1,4)-beta-D-mannosyl-(CH2)8-COOH
-
 ?
2.4.1.201 UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2)-beta-D-mannosyl-R
-
 Meleagris gallopavo UDP + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2-beta)-(N-acetyl-D-glucosaminyl-1,4)-beta-D-mannosyl-R
-
 ?
2.4.1.201 UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2)-beta-D-mannosyl-R
-
 Anas sp. UDP + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2-beta)-(N-acetyl-D-glucosaminyl-1,4)-beta-D-mannosyl-R
-
 ?
2.4.1.201 UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2)-beta-D-mannosyl-R acceptor substrate specificity Gallus gallus UDP + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2-beta)-(N-acetyl-D-glucosaminyl-1,4)-beta-D-mannosyl-R
-
 ?
2.4.1.201 UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2)-beta-D-mannosyl-R acts on bisected and non-bisected substrates Gallus gallus UDP + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2-beta)-(N-acetyl-D-glucosaminyl-1,4)-beta-D-mannosyl-R
-
 ?
2.4.1.201 UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2)-beta-D-mannosyl-R reaction in biosynthesis of penta-antennary oligosaccharides of hen ovomucoid Gallus gallus UDP + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2-beta)-(N-acetyl-D-glucosaminyl-1,4)-beta-D-mannosyl-R
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.201 UDP-GlcNAc:GlcNAcbeta1-6(GlcNAcbeta1-2)Manalpha-R (GlcNAc to Man) beta4-GlcNAc-transferase VI
-
 Gallus gallus
2.4.1.201 UDP-GlcNAc:GlcNAcbeta1-6(GlcNAcbeta1-2)Manalpha-R (GlcNAc to Man) beta4-GlcNAc-transferase VI
-
 Meleagris gallopavo
2.4.1.201 UDP-GlcNAc:GlcNAcbeta1-6(GlcNAcbeta1-2)Manalpha-R (GlcNAc to Man) beta4-GlcNAc-transferase VI
-
 Anas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.201 37
-
 assay at Gallus gallus
2.4.1.201 37
-
 assay at Meleagris gallopavo
2.4.1.201 37
-
 assay at Anas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.201 7 8.4
-
 Gallus gallus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.201 5 8.4 about half-maximal activity at pH 5.0 and about 80% of maximal activity at pH 8.4 Gallus gallus