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Literature summary extracted from

  • Forlani, G.; Parisi, B.; Nielsen, E.
    5-enol-Pyruvyl-shikimate-3-phosphate synthase from Zea Mays cultured cells. Purification and properties (1994), Plant Physiol., 105, 1107-1114.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.19 N-phosphonomethylglycine 0.006-0.008 mM, 50% inhibition, 1 mM, complete inhibition Zea mays

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.19 0.028
-
 phosphoenolpyruvate isoenzyme II Zea mays
2.5.1.19 0.029
-
 shikimate 3-phosphate isoenzyme II Zea mays
2.5.1.19 0.033
-
 phosphoenolpyruvate isoenzyme I Zea mays
2.5.1.19 0.033
-
 shikimate 3-phosphate isoenzyme I Zea mays

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.19 chloroplast isoenzymes I and II Zea mays 9507
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.19 53600
-
 1 * 53600, isoenzyme II, SDS-PAGE Zea mays
2.5.1.19 54900
-
 1 * 54900, isoenzyme I, SDS-PAGE Zea mays
2.5.1.19 58100
-
 isoenzyme II, gel filtration Zea mays
2.5.1.19 58700
-
 isoenzyme I, gel filtration Zea mays

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.19 no activity in mammals
-
-
-
2.5.1.19 Petunia x hybrida
-
-
-
2.5.1.19 Zea mays
-
 var. Black Mexican Sweet, isoenzymes I and II
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.19 ammonium sulfate, DEAE-Sephacel, cellulose phosphate column, isoenzymes I and II Zea mays

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.19 61
-
 isoenzyme I Zea mays
2.5.1.19 94.7
-
 isoenzyme II Zea mays

Storage Stability

EC Number Storage Stability Organism
2.5.1.19 -20°C, 3 months, 15% loss of activity Zea mays
2.5.1.19 0°C, 30 d, 25% loss of activity Zea mays

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.19 phosphoenolpyruvate + 3-phosphoshikimate
-
 Zea mays phosphate + 5-enolpyruvylshikimate 3-phosphate i.e. 5-O-(1-carboxyvinyl)-3-phosphoshikimate r
2.5.1.19 phosphoenolpyruvate + 3-phosphoshikimate
-
 Petunia x hybrida phosphate + 5-enolpyruvylshikimate 3-phosphate i.e. 5-O-(1-carboxyvinyl)-3-phosphoshikimate r

Subunits

EC Number Subunits Comment Organism
2.5.1.19 monomer 1 * 53600, isoenzyme II, SDS-PAGE Zea mays
2.5.1.19 monomer 1 * 54900, isoenzyme I, SDS-PAGE Zea mays

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.19 55
-
 isoenzymes I and II Zea mays

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.19 45
-
 isoenzyme I, 50% loss of activity after 20 min, isoenzyme II, 50% loss of activity after 60 min Zea mays

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.19 7.4 7.5 isoenzymes I and II Zea mays

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.19 0.00012 0.00013 N-phosphonomethylglycine isoenzymes I and II, vs. phosphoenolpyruvate Zea mays
2.5.1.19 0.0094
-
 N-phosphonomethylglycine isoenzyme I, vs. shikimate 3-phosphate Zea mays
2.5.1.19 0.012
-
 N-phosphonomethylglycine isoenzyme II, vs. shikimate 3-phosphate Zea mays