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Literature summary extracted from

  • Gruys, K.J.; Walker, M.C.; Sikorski, J.A.
    Substrate synergism and the steady-state kinetic reaction mechanism for EPSP synthase from Escherichia coli (1992), Biochemistry, 31, 5534-5544.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.19 (Z)-3-fluorophosphoenolpyruvate competitive vs. phosphoenolpyruvate at saturated shikimate 3-phosphate concentration and vice versa Escherichia coli
2.5.1.19 5-Deoxy-shikimate 3-phosphate competitive vs. shikimate 3-phosphate Escherichia coli
2.5.1.19 Carboxyallenyl phosphate strong Escherichia coli
2.5.1.19 N-phosphonomethylglycine
-
 Escherichia coli
2.5.1.19 phosphoenolpyruvate substrate inhibition Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.19 additional information
-
 additional information kinetic study Escherichia coli
2.5.1.19 0.0032 0.0036 3-phosphoshikimate
-
 Escherichia coli
2.5.1.19 0.021 0.023 phosphoenolpyruvate
-
 Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.19 Escherichia coli
-
 overproducing recombinant strain AB2829pKD501
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.5.1.19 phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate proposed mechanism Escherichia coli
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.19 phosphoenolpyruvate + 3-phosphoshikimate
-
 Escherichia coli phosphate + 5-enolpyruvylshikimate 3-phosphate i.e. 5-O-(1-carboxyvinyl)-3-phosphoshikimate r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.19 30
-
 assay at Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.19 56.6
-
 3-phosphoshikimate
-
 Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.19 0.0029
-
 (Z)-3-fluorophosphoenolpyruvate vs. shikimate 3-phosphate Escherichia coli
2.5.1.19 0.0064
-
 (Z)-3-fluorophosphoenolpyruvate vs. phosphoenolpyruvate Escherichia coli
2.5.1.19 0.0126
-
 5-Deoxy-shikimate 3-phosphate
-
 Escherichia coli