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Substrate specificity and partial purification of a stereospecific NAD- and guanidine-dependent ADP-ribosyltransferase from avian erythrocytes

Moss, J.; Stanley, S.J.; Oppenheimer, N.J.; J. Biol. Chem. 254, 8891-8894 (1979)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
2.4.2.31
highly unstable both in routine storage and in assay, can be stabilized by addition of 30-50% glycerol to the storage buffer and by addition of nontransferase protein to the assay
Meleagris gallopavo
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.2.31
glycerol
-
Meleagris gallopavo
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.2.31
0.03
-
NAD+
-
Meleagris gallopavo
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.2.31
Meleagris gallopavo
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.2.31
erythrocyte
-
Meleagris gallopavo
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.4.2.31
additional information
-
-
Meleagris gallopavo
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.2.31
agmatine + NAD+
-
638094
Meleagris gallopavo
nicotinamide + ?
-
-
-
?
2.4.2.31
arginine + NAD+
-
638094
Meleagris gallopavo
nicotinamide + ADP-ribose-L-arginine
-
-
-
?
2.4.2.31
guanidine + NAD+
-
638094
Meleagris gallopavo
nicotinamide + ?
-
-
-
?
2.4.2.31
guanidinobutyrate + NAD+
-
638094
Meleagris gallopavo
nicotinamide + ?
-
-
-
?
2.4.2.31
guanidinopropionate + NAD+
-
638094
Meleagris gallopavo
nicotinamide + ?
-
-
-
?
2.4.2.31
L-arginine methyl ester + NAD+
-
638094
Meleagris gallopavo
nicotinamide + Nomega-(ADP-D-ribosyl)-L-arginine
-
638094
Meleagris gallopavo
?
2.4.2.31
L-arginine methyl ester + NADP+
-
638094
Meleagris gallopavo
nicotinamide + N2-(2'-phospho-ADP-D-ribosyl)-L-arginine
-
-
-
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.4.2.31
NAD+
-
Meleagris gallopavo
2.4.2.31
NADP+
-
Meleagris gallopavo
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.4.2.31
NAD+
-
Meleagris gallopavo
2.4.2.31
NADP+
-
Meleagris gallopavo
General Stability (protein specific)
EC Number
General Stability
Organism
2.4.2.31
highly unstable both in routine storage and in assay, can be stabilized by addition of 30-50% glycerol to the storage buffer and by addition of nontransferase protein to the assay
Meleagris gallopavo
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.2.31
glycerol
-
Meleagris gallopavo
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.2.31
0.03
-
NAD+
-
Meleagris gallopavo
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.2.31
erythrocyte
-
Meleagris gallopavo
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.4.2.31
additional information
-
-
Meleagris gallopavo
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.2.31
agmatine + NAD+
-
638094
Meleagris gallopavo
nicotinamide + ?
-
-
-
?
2.4.2.31
arginine + NAD+
-
638094
Meleagris gallopavo
nicotinamide + ADP-ribose-L-arginine
-
-
-
?
2.4.2.31
guanidine + NAD+
-
638094
Meleagris gallopavo
nicotinamide + ?
-
-
-
?
2.4.2.31
guanidinobutyrate + NAD+
-
638094
Meleagris gallopavo
nicotinamide + ?
-
-
-
?
2.4.2.31
guanidinopropionate + NAD+
-
638094
Meleagris gallopavo
nicotinamide + ?
-
-
-
?
2.4.2.31
L-arginine methyl ester + NAD+
-
638094
Meleagris gallopavo
nicotinamide + Nomega-(ADP-D-ribosyl)-L-arginine
-
638094
Meleagris gallopavo
?
2.4.2.31
L-arginine methyl ester + NADP+
-
638094
Meleagris gallopavo
nicotinamide + N2-(2'-phospho-ADP-D-ribosyl)-L-arginine
-
-
-
?