trans-N-Deoxyribosylase: substrate specificity studies. Purine bases as acceptors

Holguin, J.; Cardinaud, R.; Eur. J. Biochem. 54, 515-520 (1975)

Data extracted from this reference:

EC Number

Inhibitors

Commentary

Organism

Structure

2.4.2.6

amino-pyrrolo-pyrimidine

12.1 mM, complete inhibition of the reaction with adenine and deoxycytidine

Lactobacillus helveticus

2.4.2.6

Hydroxypyrrolopyrimidine

0.33 mM, 66% inhibition of the reaction with adenine and deoxycytidine

Lactobacillus helveticus

2.4.2.6

imidazole

12.1 mM, 76% inhibition of the reaction with adenine and deoxycytidine

Lactobacillus helveticus

EC Number

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

Substrate Product ID

2.4.2.6

deoxycytidine + adenine

-

638043

Lactobacillus helveticus

cytosine + deoxyadenosine

-

-

-

r

2.4.2.6

thymidine + 1-deaza-8-aza-adenine

21% of substrate converted into deoxynucleoside after 2 h

638043

Lactobacillus helveticus

thymine + 2'-deoxy-1-deaza-8-aza-adenosine

-

-

-

?

2.4.2.6

thymidine + 1-deaza-adenine

6.2% of substrate converted into deoxynucleoside after 2 h

638043

Lactobacillus helveticus

thymine + 2'-deoxy-1-deaza-adenosine

-

-

-

?

2.4.2.6

thymidine + 2,6-diaminopurine

53.4% of substrate converted into deoxynucleoside after 2 h

638043

Lactobacillus helveticus

thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-2,6-diaminopurine

-

-

-

?

2.4.2.6

thymidine + 2-amino-6-methyl-mercaptopurine

-

638043

Lactobacillus helveticus

thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-2-amino-6-methylmercaptopurine

-

-

-

r

2.4.2.6

thymidine + 2-aminopurine

-

638043

Lactobacillus helveticus

thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-2-aminopurine

-

-

-

r

2.4.2.6

thymidine + 2-hydroxy-6-mercaptopurine

-

638043

Lactobacillus helveticus

additional information

2 products

638043

Lactobacillus helveticus

r

2.4.2.6

thymidine + 2-mercaptoadenine

-

638043

Lactobacillus helveticus

additional information

2 products

638043

Lactobacillus helveticus

r

2.4.2.6

thymidine + 2-mercaptopurine

-

638043

Lactobacillus helveticus

additional information

2 products

638043

Lactobacillus helveticus

r

2.4.2.6

thymidine + 3-deaza-8-aza-2-aminopurine

39.7% of substrate converted into deoxynucleoside after 2 h

638043

Lactobacillus helveticus

thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-3-deaza-8-aza-aminopurine

-

-

-

?

2.4.2.6

thymidine + 3-deaza-8-aza-adenine

3.5% of substrate converted into deoxynucleoside after 2 h

638043

Lactobacillus helveticus

thymine + 2'-deoxy-3-deaza-8-aza-adenosine

-

-

-

?

2.4.2.6

thymidine + 4-amino-5-carboxamide-imidazole

12.3% of substrate converted into deoxynucleoside after 2 h

638043

Lactobacillus helveticus

thymine + ?

-

-

-

?

2.4.2.6

thymidine + 4-amino-pyrazolopyrimidine

0.37% of substrate converted into deoxynucleoside after 2 h

638043

Lactobacillus helveticus

thymine + ?

-

-

-

?

2.4.2.6

thymidine + 5,6-dimethyl-benzimidazole

37.7% of substrate converted into deoxynucleoside after 2 h

638043

Lactobacillus helveticus

thymine + ?

-

-

-

?

2.4.2.6

thymidine + 6-benzylaminopurine

-

638043

Lactobacillus helveticus

thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-benzylaminopurine

-

-

-

r

2.4.2.6

thymidine + 6-chloropurine

-

638043

Lactobacillus helveticus

thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-chloropurine

-

-

-

r

2.4.2.6

thymidine + 6-dimethylaminopurine

-

638043

Lactobacillus helveticus

thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-dimethylaminopurine

-

-

-

r

2.4.2.6

thymidine + 6-hydrazinepurine

-

638043

Lactobacillus helveticus

additional information

several products observed

638043

Lactobacillus helveticus

r

2.4.2.6

thymidine + 6-iodopurine

65.6% of substrate converted into deoxynucleoside after 2 h

638043

Lactobacillus helveticus

thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-iodopurine

-

-

-

?

2.4.2.6

thymidine + 6-mercaptoguanine

52.1% of substrate converted into deoxynucleoside after 2 h

638043

Lactobacillus helveticus

thymine + 6-mercaptoguanosine

-

-

-

?

2.4.2.6

thymidine + 6-mercatopurine

56.5% of substrate converted into deoxynucleoside after 2 h

638043

Lactobacillus helveticus

thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-mercaptopurine

-

-

-

?

2.4.2.6

thymidine + 6-methoxypurine

-

638043

Lactobacillus helveticus

thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-methoxypurine

-

-

-

r

2.4.2.6

thymidine + 6-methylaminopurine

-

638043

Lactobacillus helveticus

thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-methylaminopurine

-

-

-

r

2.4.2.6

thymidine + 6-methylpurine

-

638043

Lactobacillus helveticus

thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-methylpurine

-

-

-

r

2.4.2.6

thymidine + 6-n-hexylaminopurine

-

638043

Lactobacillus helveticus

thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-n-hexylaminopurine

-

-

-

r

2.4.2.6

thymidine + 6-n-hexylmercaptopurine

-

638043

Lactobacillus helveticus

thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-n-hexylmercaptopurine

-

-

-

r

2.4.2.6

thymidine + 6-phenylaminopurine

-

638043

Lactobacillus helveticus

thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-phenylaminopurine

-

-

-

r

2.4.2.6

thymidine + 7-hydroxy-1,2,5-selenadiazolopyridine

29.2% of substrate converted into deoxynucleoside after 2 h

638043

Lactobacillus helveticus

thymine + ?

-

-

-

?

2.4.2.6

thymidine + 8-aza-2,6-diaminopurine

59.6% of substrate converted into deoxynucleoside after 2 h

638043

Lactobacillus helveticus

thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-8-aza-2,6-diaminopurine

-

-

-

?

2.4.2.6

thymidine + 8-azaguanine

16.0% of substrate converted into deoxynucleoside after 2 h

638043

Lactobacillus helveticus

thymine + 2'-deoxy-8-azaguanosine

-

-

-

?

2.4.2.6

thymidine + 8-azaxanthine

-

638043

Lactobacillus helveticus

thymine + 2'-deoxy-8-azaxanthosine

-

-

-

?

2.4.2.6

thymidine + adenine

-

638043

Lactobacillus helveticus

thymine + 2'-deoxyadenosine

-

-

-

r

2.4.2.6

thymidine + benzimidazole

33.1% of substrate converted into deoxynucleoside after 2 h

638043

Lactobacillus helveticus

thymine + ?

-

-

-

?

2.4.2.6

thymidine + guanine

42.3% of substrate converted into deoxynucleoside after 2 h

638043

Lactobacillus helveticus

thymine + 2'-deoxyguanosine

-

-

-

?

2.4.2.6

thymidine + hypoxanthine

-

638043

Lactobacillus helveticus

thymine + 2'-deoxyinosine

-

-

-

r

2.4.2.6

thymidine + purine

-

638043

Lactobacillus helveticus

thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-purine

-

-

-

r

2.4.2.6

thymidine + theophylline

-

638043

Lactobacillus helveticus

thymine + 2'-deoxytheophylline riboside

-

-

-

?

2.4.2.6

thymidine + xanthine

-

638043

Lactobacillus helveticus

thymine + xanthosine

-

-

-

r

EC Number

Inhibitors

Commentary

Organism

Structure

2.4.2.6

amino-pyrrolo-pyrimidine

12.1 mM, complete inhibition of the reaction with adenine and deoxycytidine

Lactobacillus helveticus

2.4.2.6

Hydroxypyrrolopyrimidine

0.33 mM, 66% inhibition of the reaction with adenine and deoxycytidine

Lactobacillus helveticus

2.4.2.6

imidazole

12.1 mM, 76% inhibition of the reaction with adenine and deoxycytidine

Lactobacillus helveticus

EC Number

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

ID

2.4.2.6

deoxycytidine + adenine

-

638043

Lactobacillus helveticus

cytosine + deoxyadenosine

-

-

-

r

2.4.2.6

thymidine + 1-deaza-8-aza-adenine

21% of substrate converted into deoxynucleoside after 2 h

638043

Lactobacillus helveticus

thymine + 2'-deoxy-1-deaza-8-aza-adenosine

-

-

-

?

2.4.2.6

thymidine + 1-deaza-adenine

6.2% of substrate converted into deoxynucleoside after 2 h

638043

Lactobacillus helveticus

thymine + 2'-deoxy-1-deaza-adenosine

-

-

-

?

2.4.2.6

thymidine + 2,6-diaminopurine

53.4% of substrate converted into deoxynucleoside after 2 h

638043

Lactobacillus helveticus

thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-2,6-diaminopurine

-

-

-

?

2.4.2.6

thymidine + 2-amino-6-methyl-mercaptopurine

-

638043

Lactobacillus helveticus

thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-2-amino-6-methylmercaptopurine

-

-

-

r

2.4.2.6

thymidine + 2-aminopurine

-

638043

Lactobacillus helveticus

thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-2-aminopurine

-

-

-

r

2.4.2.6

thymidine + 2-hydroxy-6-mercaptopurine

-

638043

Lactobacillus helveticus

additional information

2 products

638043

Lactobacillus helveticus

r

2.4.2.6

thymidine + 2-mercaptoadenine

-

638043

Lactobacillus helveticus

additional information

2 products

638043

Lactobacillus helveticus

r

2.4.2.6

thymidine + 2-mercaptopurine

-

638043

Lactobacillus helveticus

additional information

2 products

638043

Lactobacillus helveticus

r

2.4.2.6

thymidine + 3-deaza-8-aza-2-aminopurine

39.7% of substrate converted into deoxynucleoside after 2 h

638043

Lactobacillus helveticus

thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-3-deaza-8-aza-aminopurine

-

-

-

?

2.4.2.6

thymidine + 3-deaza-8-aza-adenine

3.5% of substrate converted into deoxynucleoside after 2 h

638043

Lactobacillus helveticus

thymine + 2'-deoxy-3-deaza-8-aza-adenosine

-

-

-

?

2.4.2.6

thymidine + 4-amino-5-carboxamide-imidazole

12.3% of substrate converted into deoxynucleoside after 2 h

638043

Lactobacillus helveticus

thymine + ?

-

-

-

?

2.4.2.6

thymidine + 4-amino-pyrazolopyrimidine

0.37% of substrate converted into deoxynucleoside after 2 h

638043

Lactobacillus helveticus

thymine + ?

-

-

-

?

2.4.2.6

thymidine + 5,6-dimethyl-benzimidazole

37.7% of substrate converted into deoxynucleoside after 2 h

638043

Lactobacillus helveticus

thymine + ?

-

-

-

?

2.4.2.6

thymidine + 6-benzylaminopurine

-

638043

Lactobacillus helveticus

thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-benzylaminopurine

-

-

-

r

2.4.2.6

thymidine + 6-chloropurine

-

638043

Lactobacillus helveticus

thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-chloropurine

-

-

-

r

2.4.2.6

thymidine + 6-dimethylaminopurine

-

638043

Lactobacillus helveticus

thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-dimethylaminopurine

-

-

-

r

2.4.2.6

thymidine + 6-hydrazinepurine

-

638043

Lactobacillus helveticus

additional information

several products observed

638043

Lactobacillus helveticus

r

2.4.2.6

thymidine + 6-iodopurine

65.6% of substrate converted into deoxynucleoside after 2 h

638043

Lactobacillus helveticus

thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-iodopurine

-

-

-

?

2.4.2.6

thymidine + 6-mercaptoguanine

52.1% of substrate converted into deoxynucleoside after 2 h

638043

Lactobacillus helveticus

thymine + 6-mercaptoguanosine

-

-

-

?

2.4.2.6

thymidine + 6-mercatopurine

56.5% of substrate converted into deoxynucleoside after 2 h

638043

Lactobacillus helveticus

thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-mercaptopurine

-

-

-

?

2.4.2.6

thymidine + 6-methoxypurine

-

638043

Lactobacillus helveticus

thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-methoxypurine

-

-

-

r

2.4.2.6

thymidine + 6-methylaminopurine

-

638043

Lactobacillus helveticus

thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-methylaminopurine

-

-

-

r

2.4.2.6

thymidine + 6-methylpurine

-

638043

Lactobacillus helveticus

thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-methylpurine

-

-

-

r

2.4.2.6

thymidine + 6-n-hexylaminopurine

-

638043

Lactobacillus helveticus

thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-n-hexylaminopurine

-

-

-

r

2.4.2.6

thymidine + 6-n-hexylmercaptopurine

-

638043

Lactobacillus helveticus

thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-n-hexylmercaptopurine

-

-

-

r

2.4.2.6

thymidine + 6-phenylaminopurine

-

638043

Lactobacillus helveticus

thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-phenylaminopurine

-

-

-

r

2.4.2.6

thymidine + 7-hydroxy-1,2,5-selenadiazolopyridine

29.2% of substrate converted into deoxynucleoside after 2 h

638043

Lactobacillus helveticus

thymine + ?

-

-

-

?

2.4.2.6

thymidine + 8-aza-2,6-diaminopurine

59.6% of substrate converted into deoxynucleoside after 2 h

638043

Lactobacillus helveticus

thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-8-aza-2,6-diaminopurine

-

-

-

?

2.4.2.6

thymidine + 8-azaguanine

16.0% of substrate converted into deoxynucleoside after 2 h

638043

Lactobacillus helveticus

thymine + 2'-deoxy-8-azaguanosine

-

-

-

?

2.4.2.6

thymidine + 8-azaxanthine

-

638043

Lactobacillus helveticus

thymine + 2'-deoxy-8-azaxanthosine

-

-

-

?

2.4.2.6

thymidine + adenine

-

638043

Lactobacillus helveticus

thymine + 2'-deoxyadenosine

-

-

-

r

2.4.2.6

thymidine + benzimidazole

33.1% of substrate converted into deoxynucleoside after 2 h

638043

Lactobacillus helveticus

thymine + ?

-

-

-

?

2.4.2.6

thymidine + guanine

42.3% of substrate converted into deoxynucleoside after 2 h

638043

Lactobacillus helveticus

thymine + 2'-deoxyguanosine

-

-

-

?

2.4.2.6

thymidine + hypoxanthine

-

638043

Lactobacillus helveticus

thymine + 2'-deoxyinosine

-

-

-

r

2.4.2.6

thymidine + purine

-

638043

Lactobacillus helveticus

thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-purine

-

-

-

r

2.4.2.6

thymidine + theophylline

-

638043

Lactobacillus helveticus

thymine + 2'-deoxytheophylline riboside

-

-

-

?

2.4.2.6

thymidine + xanthine

-

638043

Lactobacillus helveticus

thymine + xanthosine

-

-

-

r