BRENDA - Enzyme Database show

trans-N-Deoxyribosylase: substrate specificity studies. Purine bases as acceptors

Holguin, J.; Cardinaud, R.; Eur. J. Biochem. 54, 515-520 (1975)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.2.6
amino-pyrrolo-pyrimidine
12.1 mM, complete inhibition of the reaction with adenine and deoxycytidine
Lactobacillus helveticus
2.4.2.6
Hydroxypyrrolopyrimidine
0.33 mM, 66% inhibition of the reaction with adenine and deoxycytidine
Lactobacillus helveticus
2.4.2.6
imidazole
12.1 mM, 76% inhibition of the reaction with adenine and deoxycytidine
Lactobacillus helveticus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.2.6
Lactobacillus helveticus
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.2.6
deoxycytidine + adenine
-
638043
Lactobacillus helveticus
cytosine + deoxyadenosine
-
-
-
r
2.4.2.6
thymidine + 1-deaza-8-aza-adenine
21% of substrate converted into deoxynucleoside after 2 h
638043
Lactobacillus helveticus
thymine + 2'-deoxy-1-deaza-8-aza-adenosine
-
-
-
?
2.4.2.6
thymidine + 1-deaza-adenine
6.2% of substrate converted into deoxynucleoside after 2 h
638043
Lactobacillus helveticus
thymine + 2'-deoxy-1-deaza-adenosine
-
-
-
?
2.4.2.6
thymidine + 2,6-diaminopurine
53.4% of substrate converted into deoxynucleoside after 2 h
638043
Lactobacillus helveticus
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-2,6-diaminopurine
-
-
-
?
2.4.2.6
thymidine + 2-amino-6-methyl-mercaptopurine
-
638043
Lactobacillus helveticus
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-2-amino-6-methylmercaptopurine
-
-
-
r
2.4.2.6
thymidine + 2-aminopurine
-
638043
Lactobacillus helveticus
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-2-aminopurine
-
-
-
r
2.4.2.6
thymidine + 2-hydroxy-6-mercaptopurine
-
638043
Lactobacillus helveticus
additional information
2 products
638043
Lactobacillus helveticus
r
2.4.2.6
thymidine + 2-mercaptoadenine
-
638043
Lactobacillus helveticus
additional information
2 products
638043
Lactobacillus helveticus
r
2.4.2.6
thymidine + 2-mercaptopurine
-
638043
Lactobacillus helveticus
additional information
2 products
638043
Lactobacillus helveticus
r
2.4.2.6
thymidine + 3-deaza-8-aza-2-aminopurine
39.7% of substrate converted into deoxynucleoside after 2 h
638043
Lactobacillus helveticus
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-3-deaza-8-aza-aminopurine
-
-
-
?
2.4.2.6
thymidine + 3-deaza-8-aza-adenine
3.5% of substrate converted into deoxynucleoside after 2 h
638043
Lactobacillus helveticus
thymine + 2'-deoxy-3-deaza-8-aza-adenosine
-
-
-
?
2.4.2.6
thymidine + 4-amino-5-carboxamide-imidazole
12.3% of substrate converted into deoxynucleoside after 2 h
638043
Lactobacillus helveticus
thymine + ?
-
-
-
?
2.4.2.6
thymidine + 4-amino-pyrazolopyrimidine
0.37% of substrate converted into deoxynucleoside after 2 h
638043
Lactobacillus helveticus
thymine + ?
-
-
-
?
2.4.2.6
thymidine + 5,6-dimethyl-benzimidazole
37.7% of substrate converted into deoxynucleoside after 2 h
638043
Lactobacillus helveticus
thymine + ?
-
-
-
?
2.4.2.6
thymidine + 6-benzylaminopurine
-
638043
Lactobacillus helveticus
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-benzylaminopurine
-
-
-
r
2.4.2.6
thymidine + 6-chloropurine
-
638043
Lactobacillus helveticus
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-chloropurine
-
-
-
r
2.4.2.6
thymidine + 6-dimethylaminopurine
-
638043
Lactobacillus helveticus
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-dimethylaminopurine
-
-
-
r
2.4.2.6
thymidine + 6-hydrazinepurine
-
638043
Lactobacillus helveticus
additional information
several products observed
638043
Lactobacillus helveticus
r
2.4.2.6
thymidine + 6-iodopurine
65.6% of substrate converted into deoxynucleoside after 2 h
638043
Lactobacillus helveticus
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-iodopurine
-
-
-
?
2.4.2.6
thymidine + 6-mercaptoguanine
52.1% of substrate converted into deoxynucleoside after 2 h
638043
Lactobacillus helveticus
thymine + 6-mercaptoguanosine
-
-
-
?
2.4.2.6
thymidine + 6-mercatopurine
56.5% of substrate converted into deoxynucleoside after 2 h
638043
Lactobacillus helveticus
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-mercaptopurine
-
-
-
?
2.4.2.6
thymidine + 6-methoxypurine
-
638043
Lactobacillus helveticus
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-methoxypurine
-
-
-
r
2.4.2.6
thymidine + 6-methylaminopurine
-
638043
Lactobacillus helveticus
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-methylaminopurine
-
-
-
r
2.4.2.6
thymidine + 6-methylpurine
-
638043
Lactobacillus helveticus
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-methylpurine
-
-
-
r
2.4.2.6
thymidine + 6-n-hexylaminopurine
-
638043
Lactobacillus helveticus
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-n-hexylaminopurine
-
-
-
r
2.4.2.6
thymidine + 6-n-hexylmercaptopurine
-
638043
Lactobacillus helveticus
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-n-hexylmercaptopurine
-
-
-
r
2.4.2.6
thymidine + 6-phenylaminopurine
-
638043
Lactobacillus helveticus
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-phenylaminopurine
-
-
-
r
2.4.2.6
thymidine + 7-hydroxy-1,2,5-selenadiazolopyridine
29.2% of substrate converted into deoxynucleoside after 2 h
638043
Lactobacillus helveticus
thymine + ?
-
-
-
?
2.4.2.6
thymidine + 8-aza-2,6-diaminopurine
59.6% of substrate converted into deoxynucleoside after 2 h
638043
Lactobacillus helveticus
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-8-aza-2,6-diaminopurine
-
-
-
?
2.4.2.6
thymidine + 8-azaguanine
16.0% of substrate converted into deoxynucleoside after 2 h
638043
Lactobacillus helveticus
thymine + 2'-deoxy-8-azaguanosine
-
-
-
?
2.4.2.6
thymidine + 8-azaxanthine
-
638043
Lactobacillus helveticus
thymine + 2'-deoxy-8-azaxanthosine
-
-
-
?
2.4.2.6
thymidine + adenine
-
638043
Lactobacillus helveticus
thymine + 2'-deoxyadenosine
-
-
-
r
2.4.2.6
thymidine + benzimidazole
33.1% of substrate converted into deoxynucleoside after 2 h
638043
Lactobacillus helveticus
thymine + ?
-
-
-
?
2.4.2.6
thymidine + guanine
42.3% of substrate converted into deoxynucleoside after 2 h
638043
Lactobacillus helveticus
thymine + 2'-deoxyguanosine
-
-
-
?
2.4.2.6
thymidine + hypoxanthine
-
638043
Lactobacillus helveticus
thymine + 2'-deoxyinosine
-
-
-
r
2.4.2.6
thymidine + purine
-
638043
Lactobacillus helveticus
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-purine
-
-
-
r
2.4.2.6
thymidine + theophylline
-
638043
Lactobacillus helveticus
thymine + 2'-deoxytheophylline riboside
-
-
-
?
2.4.2.6
thymidine + xanthine
-
638043
Lactobacillus helveticus
thymine + xanthosine
-
-
-
r
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.2.6
amino-pyrrolo-pyrimidine
12.1 mM, complete inhibition of the reaction with adenine and deoxycytidine
Lactobacillus helveticus
2.4.2.6
Hydroxypyrrolopyrimidine
0.33 mM, 66% inhibition of the reaction with adenine and deoxycytidine
Lactobacillus helveticus
2.4.2.6
imidazole
12.1 mM, 76% inhibition of the reaction with adenine and deoxycytidine
Lactobacillus helveticus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.2.6
deoxycytidine + adenine
-
638043
Lactobacillus helveticus
cytosine + deoxyadenosine
-
-
-
r
2.4.2.6
thymidine + 1-deaza-8-aza-adenine
21% of substrate converted into deoxynucleoside after 2 h
638043
Lactobacillus helveticus
thymine + 2'-deoxy-1-deaza-8-aza-adenosine
-
-
-
?
2.4.2.6
thymidine + 1-deaza-adenine
6.2% of substrate converted into deoxynucleoside after 2 h
638043
Lactobacillus helveticus
thymine + 2'-deoxy-1-deaza-adenosine
-
-
-
?
2.4.2.6
thymidine + 2,6-diaminopurine
53.4% of substrate converted into deoxynucleoside after 2 h
638043
Lactobacillus helveticus
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-2,6-diaminopurine
-
-
-
?
2.4.2.6
thymidine + 2-amino-6-methyl-mercaptopurine
-
638043
Lactobacillus helveticus
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-2-amino-6-methylmercaptopurine
-
-
-
r
2.4.2.6
thymidine + 2-aminopurine
-
638043
Lactobacillus helveticus
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-2-aminopurine
-
-
-
r
2.4.2.6
thymidine + 2-hydroxy-6-mercaptopurine
-
638043
Lactobacillus helveticus
additional information
2 products
638043
Lactobacillus helveticus
r
2.4.2.6
thymidine + 2-mercaptoadenine
-
638043
Lactobacillus helveticus
additional information
2 products
638043
Lactobacillus helveticus
r
2.4.2.6
thymidine + 2-mercaptopurine
-
638043
Lactobacillus helveticus
additional information
2 products
638043
Lactobacillus helveticus
r
2.4.2.6
thymidine + 3-deaza-8-aza-2-aminopurine
39.7% of substrate converted into deoxynucleoside after 2 h
638043
Lactobacillus helveticus
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-3-deaza-8-aza-aminopurine
-
-
-
?
2.4.2.6
thymidine + 3-deaza-8-aza-adenine
3.5% of substrate converted into deoxynucleoside after 2 h
638043
Lactobacillus helveticus
thymine + 2'-deoxy-3-deaza-8-aza-adenosine
-
-
-
?
2.4.2.6
thymidine + 4-amino-5-carboxamide-imidazole
12.3% of substrate converted into deoxynucleoside after 2 h
638043
Lactobacillus helveticus
thymine + ?
-
-
-
?
2.4.2.6
thymidine + 4-amino-pyrazolopyrimidine
0.37% of substrate converted into deoxynucleoside after 2 h
638043
Lactobacillus helveticus
thymine + ?
-
-
-
?
2.4.2.6
thymidine + 5,6-dimethyl-benzimidazole
37.7% of substrate converted into deoxynucleoside after 2 h
638043
Lactobacillus helveticus
thymine + ?
-
-
-
?
2.4.2.6
thymidine + 6-benzylaminopurine
-
638043
Lactobacillus helveticus
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-benzylaminopurine
-
-
-
r
2.4.2.6
thymidine + 6-chloropurine
-
638043
Lactobacillus helveticus
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-chloropurine
-
-
-
r
2.4.2.6
thymidine + 6-dimethylaminopurine
-
638043
Lactobacillus helveticus
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-dimethylaminopurine
-
-
-
r
2.4.2.6
thymidine + 6-hydrazinepurine
-
638043
Lactobacillus helveticus
additional information
several products observed
638043
Lactobacillus helveticus
r
2.4.2.6
thymidine + 6-iodopurine
65.6% of substrate converted into deoxynucleoside after 2 h
638043
Lactobacillus helveticus
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-iodopurine
-
-
-
?
2.4.2.6
thymidine + 6-mercaptoguanine
52.1% of substrate converted into deoxynucleoside after 2 h
638043
Lactobacillus helveticus
thymine + 6-mercaptoguanosine
-
-
-
?
2.4.2.6
thymidine + 6-mercatopurine
56.5% of substrate converted into deoxynucleoside after 2 h
638043
Lactobacillus helveticus
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-mercaptopurine
-
-
-
?
2.4.2.6
thymidine + 6-methoxypurine
-
638043
Lactobacillus helveticus
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-methoxypurine
-
-
-
r
2.4.2.6
thymidine + 6-methylaminopurine
-
638043
Lactobacillus helveticus
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-methylaminopurine
-
-
-
r
2.4.2.6
thymidine + 6-methylpurine
-
638043
Lactobacillus helveticus
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-methylpurine
-
-
-
r
2.4.2.6
thymidine + 6-n-hexylaminopurine
-
638043
Lactobacillus helveticus
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-n-hexylaminopurine
-
-
-
r
2.4.2.6
thymidine + 6-n-hexylmercaptopurine
-
638043
Lactobacillus helveticus
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-n-hexylmercaptopurine
-
-
-
r
2.4.2.6
thymidine + 6-phenylaminopurine
-
638043
Lactobacillus helveticus
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-phenylaminopurine
-
-
-
r
2.4.2.6
thymidine + 7-hydroxy-1,2,5-selenadiazolopyridine
29.2% of substrate converted into deoxynucleoside after 2 h
638043
Lactobacillus helveticus
thymine + ?
-
-
-
?
2.4.2.6
thymidine + 8-aza-2,6-diaminopurine
59.6% of substrate converted into deoxynucleoside after 2 h
638043
Lactobacillus helveticus
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-8-aza-2,6-diaminopurine
-
-
-
?
2.4.2.6
thymidine + 8-azaguanine
16.0% of substrate converted into deoxynucleoside after 2 h
638043
Lactobacillus helveticus
thymine + 2'-deoxy-8-azaguanosine
-
-
-
?
2.4.2.6
thymidine + 8-azaxanthine
-
638043
Lactobacillus helveticus
thymine + 2'-deoxy-8-azaxanthosine
-
-
-
?
2.4.2.6
thymidine + adenine
-
638043
Lactobacillus helveticus
thymine + 2'-deoxyadenosine
-
-
-
r
2.4.2.6
thymidine + benzimidazole
33.1% of substrate converted into deoxynucleoside after 2 h
638043
Lactobacillus helveticus
thymine + ?
-
-
-
?
2.4.2.6
thymidine + guanine
42.3% of substrate converted into deoxynucleoside after 2 h
638043
Lactobacillus helveticus
thymine + 2'-deoxyguanosine
-
-
-
?
2.4.2.6
thymidine + hypoxanthine
-
638043
Lactobacillus helveticus
thymine + 2'-deoxyinosine
-
-
-
r
2.4.2.6
thymidine + purine
-
638043
Lactobacillus helveticus
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-purine
-
-
-
r
2.4.2.6
thymidine + theophylline
-
638043
Lactobacillus helveticus
thymine + 2'-deoxytheophylline riboside
-
-
-
?
2.4.2.6
thymidine + xanthine
-
638043
Lactobacillus helveticus
thymine + xanthosine
-
-
-
r