Nucleoside deoxyribosyltransferase from Lactobacillus helveticus
Cardinaud, R.; Methods Enzymol. 51, 446-455 (1978) 
Data extracted from this reference:
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.2.6
adenine
-
Lactobacillus helveticus
2.4.2.6
cytosine
-
Lactobacillus helveticus
2.4.2.6
deoxyadenosine
-
Lactobacillus helveticus
2.4.2.6
deoxycytidine
-
Lactobacillus helveticus
2.4.2.6
deoxyinosine
-
Lactobacillus helveticus
2.4.2.6
hypoxanthine
-
Lactobacillus helveticus
2.4.2.6
Tris
complete inhibition of deoxyribosyltransferase I at 0.1 M, pH 6.9. 62% inhibition of nucleoside deoxyribosyltransferase I at 0.8 M, pH 5.9
Lactobacillus helveticus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.2.6
0.019
-
adenine
reaction with deoxycytidine
Lactobacillus helveticus
2.4.2.6
0.041
-
deoxyinosine
-
Lactobacillus helveticus
2.4.2.6
0.086
-
hypoxanthine
-
Lactobacillus helveticus
2.4.2.6
0.09
-
deoxycytidine
-
Lactobacillus helveticus
2.4.2.6
0.12
-
deoxyadenosine
-
Lactobacillus helveticus
2.4.2.6
0.22
-
cytosine
-
Lactobacillus helveticus
2.4.2.6
0.35
-
deoxyinosine
-
Lactobacillus helveticus
2.4.2.6
0.45
-
deoxyadenosine
-
Lactobacillus helveticus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.4.2.6
86000
-
-
Lactobacillus helveticus
Organism
EC Number
Organism
UniProt
Commentary
Textmining
2.4.2.6
Lactobacillus helveticus
-
nucleoside deoxyribosyltransferase-I and nucleoside deoxyribosyltransferase-I
-
Reaction
EC Number
Reaction
Commentary
Organism
Reaction ID
2.4.2.6
2-deoxy-D-ribosyl-base1 + base2 = 2-deoxy-D-ribosyl-base2 + base1
ping-pong bi bi mechanism
Lactobacillus helveticus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2.4.2.6
5'-fluoro-5'-thymidine + adenine
deoxyribosyltransferase II
638042
Lactobacillus helveticus
5'-fluoro-5'-deoxythymine + adenosine
-
-
-
?
2.4.2.6
deoxycytidine + adenine
-
638042
Lactobacillus helveticus
cytosine + deoxyadenosine
-
638042
Lactobacillus helveticus
r
2.4.2.6
deoxyinosine + adenine
-
638042
Lactobacillus helveticus
hypoxanthine + deoxyadenosine
-
638042
Lactobacillus helveticus
r
2.4.2.6
additional information
nucleoside deoxyribosyltransferase I is strictly specific for transfer between purine bases. Nucleoside deoxyribosyltransferase II catalyzes the transfer of the deoxyribosyl moiety between purines or pyrimidines as well as from a purine to a pyrimidine
638042
Lactobacillus helveticus
?
-
-
-
-
2.4.2.6
thymidine + xanthine
-
638042
Lactobacillus helveticus
9-deoxyribosylxanthine + 7-deoxyribosylxanthine
20% 9-deoxyribosylxanthine + 80% 7-deoxyribosylxanthine
638042
Lactobacillus helveticus
?
Synonyms
EC Number
Synonyms
Commentary
Organism
2.4.2.6
nucleoside deoxyribosyltransferase I
purine nucleoside:purine deoxyribosyltransferase:strictly specific for transfer between purine bases
Lactobacillus helveticus
2.4.2.6
nucleoside deoxyribosyltransferase II
purine(pyrimidine) nucleoside:purine(pyrimidine) deoxyribosyltransferase:catalyzes the transfer of the deoxyribosyl moiety between purines or pyrimidines as well as from a purine to a pyrimidine
Lactobacillus helveticus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.2.6
5.8
-
-
Lactobacillus helveticus
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
2.4.2.6
6.5
-
optimal stability
Lactobacillus helveticus
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.4.2.6
0.021
-
adenine
-
Lactobacillus helveticus
2.4.2.6
0.039
-
adenine
-
Lactobacillus helveticus
2.4.2.6
0.079
-
hypoxanthine
-
Lactobacillus helveticus
2.4.2.6
0.092
-
deoxyadenosine
-
Lactobacillus helveticus
2.4.2.6
0.095
-
deoxycytidine
-
Lactobacillus helveticus
2.4.2.6
0.17
-
cytosine
-
Lactobacillus helveticus
2.4.2.6
0.34
-
deoxyinosine
-
Lactobacillus helveticus
2.4.2.6
0.43
-
deoxyadenosine
-
Lactobacillus helveticus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.2.6
adenine
-
Lactobacillus helveticus
2.4.2.6
cytosine
-
Lactobacillus helveticus
2.4.2.6
deoxyadenosine
-
Lactobacillus helveticus
2.4.2.6
deoxycytidine
-
Lactobacillus helveticus
2.4.2.6
deoxyinosine
-
Lactobacillus helveticus
2.4.2.6
hypoxanthine
-
Lactobacillus helveticus
2.4.2.6
Tris
complete inhibition of deoxyribosyltransferase I at 0.1 M, pH 6.9. 62% inhibition of nucleoside deoxyribosyltransferase I at 0.8 M, pH 5.9
Lactobacillus helveticus
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.4.2.6
0.021
-
adenine
-
Lactobacillus helveticus
2.4.2.6
0.039
-
adenine
-
Lactobacillus helveticus
2.4.2.6
0.079
-
hypoxanthine
-
Lactobacillus helveticus
2.4.2.6
0.092
-
deoxyadenosine
-
Lactobacillus helveticus
2.4.2.6
0.095
-
deoxycytidine
-
Lactobacillus helveticus
2.4.2.6
0.17
-
cytosine
-
Lactobacillus helveticus
2.4.2.6
0.34
-
deoxyinosine
-
Lactobacillus helveticus
2.4.2.6
0.43
-
deoxyadenosine
-
Lactobacillus helveticus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.2.6
0.019
-
adenine
reaction with deoxycytidine
Lactobacillus helveticus
2.4.2.6
0.041
-
deoxyinosine
-
Lactobacillus helveticus
2.4.2.6
0.086
-
hypoxanthine
-
Lactobacillus helveticus
2.4.2.6
0.09
-
deoxycytidine
-
Lactobacillus helveticus
2.4.2.6
0.12
-
deoxyadenosine
-
Lactobacillus helveticus
2.4.2.6
0.22
-
cytosine
-
Lactobacillus helveticus
2.4.2.6
0.35
-
deoxyinosine
-
Lactobacillus helveticus
2.4.2.6
0.45
-
deoxyadenosine
-
Lactobacillus helveticus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.4.2.6
86000
-
-
Lactobacillus helveticus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2.4.2.6
5'-fluoro-5'-thymidine + adenine
deoxyribosyltransferase II
638042
Lactobacillus helveticus
5'-fluoro-5'-deoxythymine + adenosine
-
-
-
?
2.4.2.6
deoxycytidine + adenine
-
638042
Lactobacillus helveticus
cytosine + deoxyadenosine
-
638042
Lactobacillus helveticus
r
2.4.2.6
deoxyinosine + adenine
-
638042
Lactobacillus helveticus
hypoxanthine + deoxyadenosine
-
638042
Lactobacillus helveticus
r
2.4.2.6
additional information
nucleoside deoxyribosyltransferase I is strictly specific for transfer between purine bases. Nucleoside deoxyribosyltransferase II catalyzes the transfer of the deoxyribosyl moiety between purines or pyrimidines as well as from a purine to a pyrimidine
638042
Lactobacillus helveticus
?
-
-
-
-
2.4.2.6
thymidine + xanthine
-
638042
Lactobacillus helveticus
9-deoxyribosylxanthine + 7-deoxyribosylxanthine
20% 9-deoxyribosylxanthine + 80% 7-deoxyribosylxanthine
638042
Lactobacillus helveticus
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.2.6
5.8
-
-
Lactobacillus helveticus
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
2.4.2.6
6.5
-
optimal stability
Lactobacillus helveticus