BRENDA - Enzyme Database show

Nucleoside deoxyribosyltransferase from Lactobacillus helveticus

Cardinaud, R.; Methods Enzymol. 51, 446-455 (1978)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.2.6
adenine
-
Lactobacillus helveticus
2.4.2.6
cytosine
-
Lactobacillus helveticus
2.4.2.6
deoxyadenosine
-
Lactobacillus helveticus
2.4.2.6
deoxycytidine
-
Lactobacillus helveticus
2.4.2.6
deoxyinosine
-
Lactobacillus helveticus
2.4.2.6
hypoxanthine
-
Lactobacillus helveticus
2.4.2.6
Tris
complete inhibition of deoxyribosyltransferase I at 0.1 M, pH 6.9. 62% inhibition of nucleoside deoxyribosyltransferase I at 0.8 M, pH 5.9
Lactobacillus helveticus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.2.6
0.019
-
adenine
reaction with deoxycytidine
Lactobacillus helveticus
2.4.2.6
0.041
-
deoxyinosine
-
Lactobacillus helveticus
2.4.2.6
0.086
-
hypoxanthine
-
Lactobacillus helveticus
2.4.2.6
0.09
-
deoxycytidine
-
Lactobacillus helveticus
2.4.2.6
0.12
-
deoxyadenosine
-
Lactobacillus helveticus
2.4.2.6
0.22
-
cytosine
-
Lactobacillus helveticus
2.4.2.6
0.35
-
deoxyinosine
-
Lactobacillus helveticus
2.4.2.6
0.45
-
deoxyadenosine
-
Lactobacillus helveticus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.4.2.6
86000
-
-
Lactobacillus helveticus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.2.6
Lactobacillus helveticus
-
nucleoside deoxyribosyltransferase-I and nucleoside deoxyribosyltransferase-I
-
Reaction
EC Number
Reaction
Commentary
Organism
2.4.2.6
2-deoxy-D-ribosyl-base1 + base2 = 2-deoxy-D-ribosyl-base2 + base1
ping-pong bi bi mechanism
Lactobacillus helveticus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.2.6
5'-fluoro-5'-thymidine + adenine
deoxyribosyltransferase II
638042
Lactobacillus helveticus
5'-fluoro-5'-deoxythymine + adenosine
-
-
-
?
2.4.2.6
deoxycytidine + adenine
-
638042
Lactobacillus helveticus
cytosine + deoxyadenosine
-
638042
Lactobacillus helveticus
r
2.4.2.6
deoxyinosine + adenine
-
638042
Lactobacillus helveticus
hypoxanthine + deoxyadenosine
-
638042
Lactobacillus helveticus
r
2.4.2.6
additional information
nucleoside deoxyribosyltransferase I is strictly specific for transfer between purine bases. Nucleoside deoxyribosyltransferase II catalyzes the transfer of the deoxyribosyl moiety between purines or pyrimidines as well as from a purine to a pyrimidine
638042
Lactobacillus helveticus
?
-
-
-
-
2.4.2.6
thymidine + xanthine
-
638042
Lactobacillus helveticus
9-deoxyribosylxanthine + 7-deoxyribosylxanthine
20% 9-deoxyribosylxanthine + 80% 7-deoxyribosylxanthine
638042
Lactobacillus helveticus
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.2.6
5.8
-
-
Lactobacillus helveticus
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
2.4.2.6
6.5
-
optimal stability
Lactobacillus helveticus
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.4.2.6
0.021
-
adenine
-
Lactobacillus helveticus
2.4.2.6
0.039
-
adenine
-
Lactobacillus helveticus
2.4.2.6
0.079
-
hypoxanthine
-
Lactobacillus helveticus
2.4.2.6
0.092
-
deoxyadenosine
-
Lactobacillus helveticus
2.4.2.6
0.095
-
deoxycytidine
-
Lactobacillus helveticus
2.4.2.6
0.17
-
cytosine
-
Lactobacillus helveticus
2.4.2.6
0.34
-
deoxyinosine
-
Lactobacillus helveticus
2.4.2.6
0.43
-
deoxyadenosine
-
Lactobacillus helveticus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.2.6
adenine
-
Lactobacillus helveticus
2.4.2.6
cytosine
-
Lactobacillus helveticus
2.4.2.6
deoxyadenosine
-
Lactobacillus helveticus
2.4.2.6
deoxycytidine
-
Lactobacillus helveticus
2.4.2.6
deoxyinosine
-
Lactobacillus helveticus
2.4.2.6
hypoxanthine
-
Lactobacillus helveticus
2.4.2.6
Tris
complete inhibition of deoxyribosyltransferase I at 0.1 M, pH 6.9. 62% inhibition of nucleoside deoxyribosyltransferase I at 0.8 M, pH 5.9
Lactobacillus helveticus
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.4.2.6
0.021
-
adenine
-
Lactobacillus helveticus
2.4.2.6
0.039
-
adenine
-
Lactobacillus helveticus
2.4.2.6
0.079
-
hypoxanthine
-
Lactobacillus helveticus
2.4.2.6
0.092
-
deoxyadenosine
-
Lactobacillus helveticus
2.4.2.6
0.095
-
deoxycytidine
-
Lactobacillus helveticus
2.4.2.6
0.17
-
cytosine
-
Lactobacillus helveticus
2.4.2.6
0.34
-
deoxyinosine
-
Lactobacillus helveticus
2.4.2.6
0.43
-
deoxyadenosine
-
Lactobacillus helveticus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.2.6
0.019
-
adenine
reaction with deoxycytidine
Lactobacillus helveticus
2.4.2.6
0.041
-
deoxyinosine
-
Lactobacillus helveticus
2.4.2.6
0.086
-
hypoxanthine
-
Lactobacillus helveticus
2.4.2.6
0.09
-
deoxycytidine
-
Lactobacillus helveticus
2.4.2.6
0.12
-
deoxyadenosine
-
Lactobacillus helveticus
2.4.2.6
0.22
-
cytosine
-
Lactobacillus helveticus
2.4.2.6
0.35
-
deoxyinosine
-
Lactobacillus helveticus
2.4.2.6
0.45
-
deoxyadenosine
-
Lactobacillus helveticus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.4.2.6
86000
-
-
Lactobacillus helveticus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.2.6
5'-fluoro-5'-thymidine + adenine
deoxyribosyltransferase II
638042
Lactobacillus helveticus
5'-fluoro-5'-deoxythymine + adenosine
-
-
-
?
2.4.2.6
deoxycytidine + adenine
-
638042
Lactobacillus helveticus
cytosine + deoxyadenosine
-
638042
Lactobacillus helveticus
r
2.4.2.6
deoxyinosine + adenine
-
638042
Lactobacillus helveticus
hypoxanthine + deoxyadenosine
-
638042
Lactobacillus helveticus
r
2.4.2.6
additional information
nucleoside deoxyribosyltransferase I is strictly specific for transfer between purine bases. Nucleoside deoxyribosyltransferase II catalyzes the transfer of the deoxyribosyl moiety between purines or pyrimidines as well as from a purine to a pyrimidine
638042
Lactobacillus helveticus
?
-
-
-
-
2.4.2.6
thymidine + xanthine
-
638042
Lactobacillus helveticus
9-deoxyribosylxanthine + 7-deoxyribosylxanthine
20% 9-deoxyribosylxanthine + 80% 7-deoxyribosylxanthine
638042
Lactobacillus helveticus
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.2.6
5.8
-
-
Lactobacillus helveticus
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
2.4.2.6
6.5
-
optimal stability
Lactobacillus helveticus