BRENDA - Enzyme Database show

Purification and properties of trans-N-deoxyribosylase

Roush, A.H.; Betz, R.F.; J. Biol. Chem. 233, 261-266 (1958)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.2.6
Tris
-
Lactobacillus helveticus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.2.6
Lactobacillus helveticus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.4.2.6
-
Lactobacillus helveticus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.2.6
deoxyadenosine + cytosine
-
638040
Lactobacillus helveticus
adenine + deoxycytidine
-
-
-
r
2.4.2.6
deoxyadenosine + hypoxanthine
-
638040
Lactobacillus helveticus
adenine + deoxyinosine + 7-(beta-D-2'-deoxyribufuranosyl)hypoxanthine
-
-
-
r
2.4.2.6
deoxyadenosine + thymine
-
638040
Lactobacillus helveticus
adenine + thymidine
-
-
-
r
2.4.2.6
deoxyadenosine + uracil
-
638040
Lactobacillus helveticus
adenine + deoxyuridine
-
-
-
r
2.4.2.6
deoxyadenosine + xanthine
-
638040
Lactobacillus helveticus
adenine + deoxyxanthosine
-
-
-
r
2.4.2.6
deoxycytidine + adenine
-
638040
Lactobacillus helveticus
cytosine + deoxyadenosine
-
638040
Lactobacillus helveticus
r
2.4.2.6
deoxycytidine + cytosine
-
638040
Lactobacillus helveticus
cytosine + deoxycytidine
-
-
-
?
2.4.2.6
deoxycytidine + guanine
-
638040
Lactobacillus helveticus
cytosine + deoxyguanosine
-
-
-
r
2.4.2.6
deoxycytidine + hypoxanthine
-
638040
Lactobacillus helveticus
cytosine + deoxyinosine
-
-
-
r
2.4.2.6
deoxycytidine + thymine
-
638040
Lactobacillus helveticus
cytosine + thymidine
-
-
-
r
2.4.2.6
deoxycytidine + xanthine
-
638040
Lactobacillus helveticus
cytosine + xanthosine
-
-
-
r
2.4.2.6
deoxyguanosine + adenine
-
638040
Lactobacillus helveticus
guanine + deoxyadenosine
-
-
-
r
2.4.2.6
deoxyguanosine + cytosine
-
638040
Lactobacillus helveticus
guanine + deoxycytidine
-
-
-
r
2.4.2.6
deoxyguanosine + hypoxanthine
-
638040
Lactobacillus helveticus
guanine + deoxyinosine
-
-
-
r
2.4.2.6
deoxyguanosine + thymine
-
638040
Lactobacillus helveticus
guanine + thymidine
-
-
-
r
2.4.2.6
deoxyguanosine + uracil
-
638040
Lactobacillus helveticus
guanine + deoxyuridine
-
-
-
r
2.4.2.6
deoxyguanosine + xanthine
-
638040
Lactobacillus helveticus
guanine + deoxyxanthine
-
-
-
r
2.4.2.6
deoxyinosine + 8-azaguanine
-
638040
Lactobacillus helveticus
hypoxanthine + deoxy-8-azaguanosine
-
-
-
r
2.4.2.6
deoxyinosine + 8-azaxanthine
-
638040
Lactobacillus helveticus
hypoxanthine + deoxy-8-azaxanthosine
-
-
-
r
2.4.2.6
deoxyinosine + adenine
-
638040
Lactobacillus helveticus
hypoxanthine + deoxyadenosine
-
638040
Lactobacillus helveticus
r
2.4.2.6
deoxyinosine + cytosine
-
638040
Lactobacillus helveticus
hypoxanthine + deoxycytidine
-
-
-
r
2.4.2.6
deoxyinosine + guanine
-
638040
Lactobacillus helveticus
hypoxanthine + deoxyguanosine
-
638040
Lactobacillus helveticus
r
2.4.2.6
deoxyinosine + thymine
-
638040
Lactobacillus helveticus
hypoxanthine + thymidine
-
-
-
r
2.4.2.6
deoxyinosine + uracil
-
638040
Lactobacillus helveticus
hypoxanthine + deoxyuridine
-
-
-
r
2.4.2.6
deoxyinosine + uric acid
-
638040
Lactobacillus helveticus
hypoxanthine + 9-(2-deoxy-beta-D-ribofuranosyl)-7,9-dihydro-3H-purine-2,6,8-trione
-
-
-
r
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.4.2.6
55
-
pH 6.3, 10 min, stable below
Lactobacillus helveticus
2.4.2.6
90
-
pH 6.3, 10 min, 75% loss of activity
Lactobacillus helveticus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.2.6
5.8
-
-
Lactobacillus helveticus
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
2.4.2.6
4
-
61°C, 30 min, about 30% loss of activity
Lactobacillus helveticus
2.4.2.6
6.5
-
61°C, 30 min, maximal stability
Lactobacillus helveticus
2.4.2.6
8
-
61°C, 30 min, about 15% loss of activity
Lactobacillus helveticus
2.4.2.6
8.5
-
61°C, 30 min, about 70% of maximal activity
Lactobacillus helveticus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.2.6
Tris
-
Lactobacillus helveticus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.4.2.6
-
Lactobacillus helveticus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.2.6
deoxyadenosine + cytosine
-
638040
Lactobacillus helveticus
adenine + deoxycytidine
-
-
-
r
2.4.2.6
deoxyadenosine + hypoxanthine
-
638040
Lactobacillus helveticus
adenine + deoxyinosine + 7-(beta-D-2'-deoxyribufuranosyl)hypoxanthine
-
-
-
r
2.4.2.6
deoxyadenosine + thymine
-
638040
Lactobacillus helveticus
adenine + thymidine
-
-
-
r
2.4.2.6
deoxyadenosine + uracil
-
638040
Lactobacillus helveticus
adenine + deoxyuridine
-
-
-
r
2.4.2.6
deoxyadenosine + xanthine
-
638040
Lactobacillus helveticus
adenine + deoxyxanthosine
-
-
-
r
2.4.2.6
deoxycytidine + adenine
-
638040
Lactobacillus helveticus
cytosine + deoxyadenosine
-
638040
Lactobacillus helveticus
r
2.4.2.6
deoxycytidine + cytosine
-
638040
Lactobacillus helveticus
cytosine + deoxycytidine
-
-
-
?
2.4.2.6
deoxycytidine + guanine
-
638040
Lactobacillus helveticus
cytosine + deoxyguanosine
-
-
-
r
2.4.2.6
deoxycytidine + hypoxanthine
-
638040
Lactobacillus helveticus
cytosine + deoxyinosine
-
-
-
r
2.4.2.6
deoxycytidine + thymine
-
638040
Lactobacillus helveticus
cytosine + thymidine
-
-
-
r
2.4.2.6
deoxycytidine + xanthine
-
638040
Lactobacillus helveticus
cytosine + xanthosine
-
-
-
r
2.4.2.6
deoxyguanosine + adenine
-
638040
Lactobacillus helveticus
guanine + deoxyadenosine
-
-
-
r
2.4.2.6
deoxyguanosine + cytosine
-
638040
Lactobacillus helveticus
guanine + deoxycytidine
-
-
-
r
2.4.2.6
deoxyguanosine + hypoxanthine
-
638040
Lactobacillus helveticus
guanine + deoxyinosine
-
-
-
r
2.4.2.6
deoxyguanosine + thymine
-
638040
Lactobacillus helveticus
guanine + thymidine
-
-
-
r
2.4.2.6
deoxyguanosine + uracil
-
638040
Lactobacillus helveticus
guanine + deoxyuridine
-
-
-
r
2.4.2.6
deoxyguanosine + xanthine
-
638040
Lactobacillus helveticus
guanine + deoxyxanthine
-
-
-
r
2.4.2.6
deoxyinosine + 8-azaguanine
-
638040
Lactobacillus helveticus
hypoxanthine + deoxy-8-azaguanosine
-
-
-
r
2.4.2.6
deoxyinosine + 8-azaxanthine
-
638040
Lactobacillus helveticus
hypoxanthine + deoxy-8-azaxanthosine
-
-
-
r
2.4.2.6
deoxyinosine + adenine
-
638040
Lactobacillus helveticus
hypoxanthine + deoxyadenosine
-
638040
Lactobacillus helveticus
r
2.4.2.6
deoxyinosine + cytosine
-
638040
Lactobacillus helveticus
hypoxanthine + deoxycytidine
-
-
-
r
2.4.2.6
deoxyinosine + guanine
-
638040
Lactobacillus helveticus
hypoxanthine + deoxyguanosine
-
638040
Lactobacillus helveticus
r
2.4.2.6
deoxyinosine + thymine
-
638040
Lactobacillus helveticus
hypoxanthine + thymidine
-
-
-
r
2.4.2.6
deoxyinosine + uracil
-
638040
Lactobacillus helveticus
hypoxanthine + deoxyuridine
-
-
-
r
2.4.2.6
deoxyinosine + uric acid
-
638040
Lactobacillus helveticus
hypoxanthine + 9-(2-deoxy-beta-D-ribofuranosyl)-7,9-dihydro-3H-purine-2,6,8-trione
-
-
-
r
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.4.2.6
55
-
pH 6.3, 10 min, stable below
Lactobacillus helveticus
2.4.2.6
90
-
pH 6.3, 10 min, 75% loss of activity
Lactobacillus helveticus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.2.6
5.8
-
-
Lactobacillus helveticus
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
2.4.2.6
4
-
61°C, 30 min, about 30% loss of activity
Lactobacillus helveticus
2.4.2.6
6.5
-
61°C, 30 min, maximal stability
Lactobacillus helveticus
2.4.2.6
8
-
61°C, 30 min, about 15% loss of activity
Lactobacillus helveticus
2.4.2.6
8.5
-
61°C, 30 min, about 70% of maximal activity
Lactobacillus helveticus