BRENDA - Enzyme Database show

Purification and characterization of poly (ADP-ribose) synthetase from human placenta

Ushiro, H.; Yokoyama, Y.; Shizuta, Y.; J. Biol. Chem. 262, 2352-2357 (1987)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.4.2.30
DNA
absolute requirement
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.2.30
3-aminobenzamide
1 mM, 99% inhibition
Homo sapiens
2.4.2.30
3-aminobenzoic acid
1 mM, 12% inhibition
Homo sapiens
2.4.2.30
Cu2+
-
Homo sapiens
2.4.2.30
Hg2+
-
Homo sapiens
2.4.2.30
nicotinamide
1 mM, 93% inhibition
Homo sapiens
2.4.2.30
PCMB
complete
Homo sapiens
2.4.2.30
Zn2+
-
Homo sapiens
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.2.30
Ba2+
enhances activity
Homo sapiens
2.4.2.30
Mg2+
enhances both the automodification and poly(ADP-ribosyl)ation of histone H1
Homo sapiens
2.4.2.30
Sr2+
enhances activity
Homo sapiens
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.4.2.30
116000
-
1 * 116000, SDS-PAGE
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.2.30
Homo sapiens
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.4.2.30
-
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.2.30
placenta
-
Homo sapiens
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.4.2.30
1.02
-
-
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.2.30
NAD+ + (ADP-D-ribosyl)n-acceptor
poly(ADP-ribosyl)ation of histone H1
637991
Homo sapiens
nicotinamide + (ADP-D-ribosyl)n+1-acceptor
-
-
-
?
2.4.2.30
NAD+ + (ADP-D-ribosyl)n-acceptor
catalyzes poly(ADP-ribosyl)ation of the synthetase itself, automodification
637991
Homo sapiens
nicotinamide + (ADP-D-ribosyl)n+1-acceptor
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
2.4.2.30
monomer
1 * 116000, SDS-PAGE
Homo sapiens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.2.30
8.5
-
reaction with histone in presence of DNA, 0.1 M glycine-NaOH buffer
Homo sapiens
2.4.2.30
8.7
-
reaction with histone in presence of DNA, 0.1 M Tris-HCl buffer
Homo sapiens
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.4.2.30
DNA
absolute requirement
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.2.30
3-aminobenzamide
1 mM, 99% inhibition
Homo sapiens
2.4.2.30
3-aminobenzoic acid
1 mM, 12% inhibition
Homo sapiens
2.4.2.30
Cu2+
-
Homo sapiens
2.4.2.30
Hg2+
-
Homo sapiens
2.4.2.30
nicotinamide
1 mM, 93% inhibition
Homo sapiens
2.4.2.30
PCMB
complete
Homo sapiens
2.4.2.30
Zn2+
-
Homo sapiens
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.2.30
Ba2+
enhances activity
Homo sapiens
2.4.2.30
Mg2+
enhances both the automodification and poly(ADP-ribosyl)ation of histone H1
Homo sapiens
2.4.2.30
Sr2+
enhances activity
Homo sapiens
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.4.2.30
116000
-
1 * 116000, SDS-PAGE
Homo sapiens
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.4.2.30
-
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.2.30
placenta
-
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.4.2.30
1.02
-
-
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.2.30
NAD+ + (ADP-D-ribosyl)n-acceptor
poly(ADP-ribosyl)ation of histone H1
637991
Homo sapiens
nicotinamide + (ADP-D-ribosyl)n+1-acceptor
-
-
-
?
2.4.2.30
NAD+ + (ADP-D-ribosyl)n-acceptor
catalyzes poly(ADP-ribosyl)ation of the synthetase itself, automodification
637991
Homo sapiens
nicotinamide + (ADP-D-ribosyl)n+1-acceptor
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.4.2.30
monomer
1 * 116000, SDS-PAGE
Homo sapiens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.2.30
8.5
-
reaction with histone in presence of DNA, 0.1 M glycine-NaOH buffer
Homo sapiens
2.4.2.30
8.7
-
reaction with histone in presence of DNA, 0.1 M Tris-HCl buffer
Homo sapiens