Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kim, Y.K.; Kitaoka, M.; Krishnareddy, M.; Mori, Y.; Hayashi, K.
    Kinetic studies of a recombinant cellobiose phosphorylase (CBP) of the Clostridium thermocellum YM4 strain expressed in Escherichia coli (2002), J. Biochem., 132, 197-203.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.20 expressed in Escherichia coli Acetivibrio thermocellus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.20 D-glucose substrate inhibition, competitive with glucose-1-phosphate Acetivibrio thermocellus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.20 0.97
-
 D-glucose
-
 Acetivibrio thermocellus
2.4.1.20 5.9
-
 D-glucosamine
-
 Acetivibrio thermocellus
2.4.1.20 6.1
-
 6-deoxy-D-glucose
-
 Acetivibrio thermocellus
2.4.1.20 21.4
-
 D-xylose
-
 Acetivibrio thermocellus
2.4.1.20 56.9
-
 2-deoxy-D-glucose
-
 Acetivibrio thermocellus
2.4.1.20 131
-
 D-mannose
-
 Acetivibrio thermocellus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.20 92000
-
 SDS-PAGE, native PAGE Acetivibrio thermocellus
2.4.1.20 93760
-
 calculated from DNA-sequence Acetivibrio thermocellus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.20 cellobiose + phosphate Acetivibrio thermocellus
-
 alpha-D-glucose 1-phosphate + D-glucose
-
 ?
2.4.1.20 cellobiose + phosphate Acetivibrio thermocellus YM4
-
 alpha-D-glucose 1-phosphate + D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.20 Acetivibrio thermocellus
-
-
-
2.4.1.20 Acetivibrio thermocellus YM4
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.20 recombinant enzyme from E. coli Acetivibrio thermocellus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.20 alpha-D-glucose 1-phosphate + 2-deoxy-D-glucose
-
 Acetivibrio thermocellus ?
-
 ?
2.4.1.20 alpha-D-glucose 1-phosphate + 2-deoxy-D-glucose
-
 Acetivibrio thermocellus YM4 ?
-
 ?
2.4.1.20 alpha-D-glucose 1-phosphate + 6-deoxy-D-glucose
-
 Acetivibrio thermocellus ?
-
 ?
2.4.1.20 alpha-D-glucose 1-phosphate + 6-deoxy-D-glucose
-
 Acetivibrio thermocellus YM4 ?
-
 ?
2.4.1.20 alpha-D-glucose 1-phosphate + D-glucosamine
-
 Acetivibrio thermocellus ?
-
 ?
2.4.1.20 alpha-D-glucose 1-phosphate + D-mannose
-
 Acetivibrio thermocellus ?
-
 ?
2.4.1.20 alpha-D-glucose 1-phosphate + D-xylose no activity with L-isomer Acetivibrio thermocellus ?
-
 ?
2.4.1.20 cellobiose + phosphate
-
 Acetivibrio thermocellus alpha-D-glucose 1-phosphate + D-glucose
-
 ?
2.4.1.20 cellobiose + phosphate ordered bi-bi mechanism Acetivibrio thermocellus alpha-D-glucose 1-phosphate + D-glucose
-
 ?
2.4.1.20 cellobiose + phosphate beta-cellobiose preferred over alpha-anomer Acetivibrio thermocellus alpha-D-glucose 1-phosphate + D-glucose
-
 ?
2.4.1.20 cellobiose + phosphate
-
 Acetivibrio thermocellus YM4 alpha-D-glucose 1-phosphate + D-glucose
-
 ?
2.4.1.20 cellobiose + phosphate ordered bi-bi mechanism Acetivibrio thermocellus YM4 alpha-D-glucose 1-phosphate + D-glucose
-
 ?
2.4.1.20 cellobiose + phosphate beta-cellobiose preferred over alpha-anomer Acetivibrio thermocellus YM4 alpha-D-glucose 1-phosphate + D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.20 monomer 1 * 92000, SDS-PAGE, native PAGE Acetivibrio thermocellus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.20 7.7
-
 D-glucosamine
-
 Acetivibrio thermocellus
2.4.1.20 9.3
-
 D-mannose
-
 Acetivibrio thermocellus
2.4.1.20 12.2
-
 D-glucose
-
 Acetivibrio thermocellus
2.4.1.20 18.3
-
 2-deoxy-D-glucose
-
 Acetivibrio thermocellus
2.4.1.20 44.3
-
 D-xylose
-
 Acetivibrio thermocellus
2.4.1.20 59.5
-
 6-deoxy-D-glucose
-
 Acetivibrio thermocellus