EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.1.20 | expressed in Escherichia coli | Thermotoga maritima |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.20 | D-glucose | substrate inhibition, competitive with glucose-1-phosphate | Thermotoga maritima | |
2.4.1.20 | D-glucose 1-phosphate | - |
Thermotoga maritima |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.20 | 0.15 | - |
phosphate | - |
Thermotoga maritima | |
2.4.1.20 | 0.29 | - |
cellobiose | - |
Thermotoga maritima | |
2.4.1.20 | 0.69 | - |
D-glucose | - |
Thermotoga maritima | |
2.4.1.20 | 4.1 | - |
6-deoxy-D-glucose | - |
Thermotoga maritima | |
2.4.1.20 | 5.7 | - |
D-glucosamine | - |
Thermotoga maritima | |
2.4.1.20 | 14 | - |
D-xylose | - |
Thermotoga maritima | |
2.4.1.20 | 47 | - |
2-deoxy-D-glucose | - |
Thermotoga maritima | |
2.4.1.20 | 67 | - |
D-mannose | - |
Thermotoga maritima |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.4.1.20 | 85000 | - |
SDS-PAGE | Thermotoga maritima |
2.4.1.20 | 94320 | - |
calculated from DNA-sequence | Thermotoga maritima |
2.4.1.20 | 150000 | - |
gel filtration | Thermotoga maritima |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.20 | cellobiose + phosphate | Thermotoga maritima | - |
alpha-D-glucose 1-phosphate + D-glucose | - |
? | |
2.4.1.20 | cellobiose + phosphate | Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | - |
alpha-D-glucose 1-phosphate + D-glucose | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.20 | Thermotoga maritima | - |
- |
- |
2.4.1.20 | Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.1.20 | - |
Thermotoga maritima |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.4.1.20 | 2.66 | - |
purified enzyme | Thermotoga maritima |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.20 | alpha-D-glucose 1-phosphate + 2-deoxy-D-glucose | - |
Thermotoga maritima | ? | - |
? | |
2.4.1.20 | alpha-D-glucose 1-phosphate + 2-deoxy-D-glucose | - |
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | ? | - |
? | |
2.4.1.20 | alpha-D-glucose 1-phosphate + 6-deoxy-D-glucose | - |
Thermotoga maritima | ? | - |
? | |
2.4.1.20 | alpha-D-glucose 1-phosphate + 6-deoxy-D-glucose | - |
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | ? | - |
? | |
2.4.1.20 | alpha-D-glucose 1-phosphate + D-glucosamine | - |
Thermotoga maritima | ? | - |
? | |
2.4.1.20 | alpha-D-glucose 1-phosphate + D-mannose | - |
Thermotoga maritima | ? | - |
? | |
2.4.1.20 | alpha-D-glucose 1-phosphate + D-xylose | no activity with L-isomer | Thermotoga maritima | ? | - |
? | |
2.4.1.20 | alpha-D-glucose 1-phosphate + D-xylose | higher specific activity than with D-glucose as acceptor | Thermotoga maritima | ? | - |
? | |
2.4.1.20 | cellobiose + phosphate | - |
Thermotoga maritima | alpha-D-glucose 1-phosphate + D-glucose | - |
? | |
2.4.1.20 | cellobiose + phosphate | random ordered bi-bi mechanism | Thermotoga maritima | alpha-D-glucose 1-phosphate + D-glucose | - |
? | |
2.4.1.20 | cellobiose + phosphate | sequential bi-bi mechanism | Thermotoga maritima | alpha-D-glucose 1-phosphate + D-glucose | - |
? | |
2.4.1.20 | cellobiose + phosphate | - |
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | alpha-D-glucose 1-phosphate + D-glucose | - |
? | |
2.4.1.20 | cellobiose + phosphate | random ordered bi-bi mechanism | Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | alpha-D-glucose 1-phosphate + D-glucose | - |
? | |
2.4.1.20 | cellobiose + phosphate | sequential bi-bi mechanism | Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | alpha-D-glucose 1-phosphate + D-glucose | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.4.1.20 | dimer | 2 * 85000, gel filtration, SDS-PAGE | Thermotoga maritima |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.20 | 80 | - |
- |
Thermotoga maritima |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.20 | 40 | 95 | - |
Thermotoga maritima |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.20 | 70 | - |
less than 10% loss of activity after heating for 30 min at pH 6-8 | Thermotoga maritima |
2.4.1.20 | 90 | - |
25% loss of activity after heating for 30 min at pH 6-8 | Thermotoga maritima |
2.4.1.20 | 100 | - |
complete loss of activity after heating for 30 min at pH 6-8 | Thermotoga maritima |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.20 | 4.4 | - |
D-mannose | - |
Thermotoga maritima | |
2.4.1.20 | 5.2 | - |
D-glucosamine | - |
Thermotoga maritima | |
2.4.1.20 | 5.4 | - |
cellobiose | - |
Thermotoga maritima | |
2.4.1.20 | 8 | - |
D-glucose | - |
Thermotoga maritima | |
2.4.1.20 | 16 | - |
2-deoxy-D-glucose | - |
Thermotoga maritima | |
2.4.1.20 | 17 | - |
6-deoxy-D-glucose | - |
Thermotoga maritima | |
2.4.1.20 | 40 | - |
D-xylose | - |
Thermotoga maritima |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.20 | 6.2 | - |
- |
Thermotoga maritima |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.20 | 4 | 9 | - |
Thermotoga maritima |