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Literature summary extracted from

  • von Rad, U.; Huttl, R.; Lottspeich, F.; Gierl, A.; Frey, M.
    Two glucosyltransferases are involved in detoxification of benzoxazinoids in maize (2001), Plant J., 28, 633-642.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.202 expressed in Escherichia coli Zea mays
2.4.1.202 transfected into Arabidopsis Zea mays

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.202 0.061
-
 2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one BX8-gene encoded protein Zea mays
2.4.1.202 0.071
-
 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one BX9-gene encoded protein Zea mays
2.4.1.202 0.081
-
 UDP-glucose BX8-gene encoded protein Zea mays
2.4.1.202 0.081
-
 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one BX8-gene encoded protein Zea mays
2.4.1.202 0.096
-
 UDP-glucose BX9-gene encoded protein Zea mays
2.4.1.202 1.3
-
 2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one BX9-gene encoded protein Zea mays

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.202 48000
-
 gel filtration Zea mays

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.202 UDP-glucose + 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one Zea mays involved in detoxification of benzoxazinoids UDP + 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one 2-D-glucoside
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.202 Zea mays
-
 two different enzymes with different substrate specificities, genes Bx8 and Bx9
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.202 0.5
-
 2-hydroxy-2H-1,4-benzoxazin-3(4H)-one, BX8-gene encoded protein Zea mays
2.4.1.202 0.5
-
 2-hydroxy-2H-1,4-benzoxazin-3(4H)-one, BX9-gene encoded protein Zea mays
2.4.1.202 1.4
-
 2-hydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one, BX8-gene encoded protein Zea mays
2.4.1.202 2.6
-
 2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one as substrate, BX9-gene encoded protein Zea mays
2.4.1.202 6
-
 2-hydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one, BX9-gene encoded protein Zea mays
2.4.1.202 13
-
 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one as substrate, BX9-gene encoded protein Zea mays
2.4.1.202 13.4
-
 2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one as substrate, BX8-gene encoded protein Zea mays
2.4.1.202 24
-
 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one as substrate, BX8-gene encoded protein Zea mays

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.202 UDP-glucose + 2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one
-
 Zea mays UDP + 2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one 2-D-glucoside
-
 ?
2.4.1.202 UDP-glucose + 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one
-
 Zea mays UDP + 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one 2-D-glucoside
-
 ?
2.4.1.202 UDP-glucose + 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one involved in detoxification of benzoxazinoids Zea mays UDP + 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one 2-D-glucoside
-
 ?
2.4.1.202 UDP-glucose + 2-hydroxy-2H-1,4-benzoxazin-3(4H)-one low glucosylation efficiency Zea mays UDP + 2-hydroxy-2H-1,4-benzoxazin-3(4H)-one 2-D-glucoside
-
 ?
2.4.1.202 UDP-glucose + 2-hydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one
-
 Zea mays UDP + 2-hydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one 2-D-glucoside
-
 ?