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Literature summary extracted from

  • Warnecke, D.C.; Baltrusch, M.; Buck, F.; Wolter, F.P.; Heinz, E.
    UDP-glucose:sterol glucosyltransferase: cloning and functional expression in Escherichia coli (1997), Plant Mol. Biol., 35, 597-603.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.173 expression in Escherichia coli Arabidopsis thaliana
2.4.1.173 expression in Escherichia coli Avena sativa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.173 66000
-
 cloned, N-terminal peptide of 133 amino acid could be cleaved resulting in a molecular weight of 52000 in agreement with 56000 of the purified enzyme Avena sativa
2.4.1.173 69000
-
 cloned, calculated molecular mass Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.173 Arabidopsis thaliana Q9M8Z7 ugt80A2
-
2.4.1.173 Avena sativa O22678 ugt80A1
-
2.4.1.173 Avena sativa ugt80A1 O22678 ugt80A1
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.173 flower cDNA library Arabidopsis thaliana
-
2.4.1.173 leaf cDNA library Arabidopsis thaliana
-
2.4.1.173 root cDNA library Arabidopsis thaliana
-
2.4.1.173 shoot cDNA library Arabidopsis thaliana
-
2.4.1.173 shoot cloned from Avena sativa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.173 UDP-glucose + cholesterol cloned enzyme exhibits equal activity to the purified protein Arabidopsis thaliana UDP + cholesterol 3-beta-D-glucoside
-
 ?
2.4.1.173 UDP-glucose + cholesterol cloned enzyme exhibits equal activity to the purified protein Avena sativa UDP + cholesterol 3-beta-D-glucoside
-
 ?
2.4.1.173 UDP-glucose + cholesterol cloned enzyme exhibits equal activity to the purified protein Avena sativa ugt80A1 UDP + cholesterol 3-beta-D-glucoside
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.173 30
-
 assay at Arabidopsis thaliana
2.4.1.173 30
-
 assay at Avena sativa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.173 8
-
-
 Arabidopsis thaliana
2.4.1.173 8
-
 Tris-HCl 100 mM, dithiothreitol 1 mM, Triton X-100 0.2% Avena sativa