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Literature summary extracted from

  • Warnecke, D.C.; Heinz, E.
    Purification of a membrane-bound UDP-glucose:sterol beta-D-glucosyltransferase based on its solubility in diethyl ether (1994), Plant Physiol., 105, 1067-1073.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.173 4-thio-UDP 50% of inhibition at 160 uM Avena sativa
2.4.1.173 UDP 50% of inhibition at 70 uM Avena sativa
2.4.1.173 UDP-mannose 50% of inhibition at 40 uM Avena sativa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.173 0.24 0.54 sitosterol
-
 Avena sativa
2.4.1.173 0.34
-
 UDP-glucose
-
 Avena sativa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.173 microsome
-
 Avena sativa
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.173 56000
-
 purification by Q-Sepharose fast flow column, Hitrap blue column and SDS-PAGE Avena sativa

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.173 Avena sativa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.173 purification about 12500fold by microsomes, extraction with diethyl ether, Q-Sepharose fast flow column, Hitrap blue column and SDS-PAGE Avena sativa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.173 shoot etiolated Avena sativa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.173 additional information UDP-glucose is an effective glucose donor but not so UDP-mannose, UDP-galactose or UMP-glucose Avena sativa ?
-
 ?
2.4.1.173 UDP-glucose + cholesterol with sitosterol, best substrates Avena sativa UDP + cholesterol 3-beta-D-glucoside
-
 ?
2.4.1.173 UDP-glucose + ergosterol 69% of the activity with sitosterol Avena sativa UDP + ergosterol 3-beta-D-glucoside
-
 ?
2.4.1.173 UDP-glucose + sitosterol with cholesterol, best substrates Avena sativa UDP + sitosterol 3-beta-D-glucoside
-
 ?
2.4.1.173 UDP-glucose + stigmasterol 63% of the activity with sitosterol Avena sativa UDP + stigmasterol 3-beta-D-glucoside
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.173 30
-
 assay at Avena sativa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.173 8.5
-
 Tris-HCl 60 mM, Triton X-100 0.2%, dithiothreitol 2.5 mM Avena sativa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.1.173 0.047
-
 UDP
-
 Avena sativa