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Literature summary extracted from

  • Rajavel, M.; Lalo, D.; Gross, J.W.; Grubmeyer, C.
    Conversion of a cosubstrate to an inhibitor. Phosphorylation mutants of nicotinic acid phosphoribosyltransferase (1998), Biochemistry, 37, 4181-4188.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.4.21 expression in Escherichia coli Saccharomyces cerevisiae

General Stability

EC Number General Stability Organism
6.3.4.21 first order activity loss in the presence of 1/200 trypsin/enzyme Salmonella enterica subsp. enterica serovar Typhimurium
6.3.4.21 sensitive to trypsin, partial proteolysis, 4fold increase in t1/2 for inactivation in the presence of 1 mM MgATP2- Salmonella enterica subsp. enterica serovar Typhimurium

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.4.21 ATP
-
 Saccharomyces cerevisiae
6.3.4.21 ATP 3 mM, competitive inhibition of nicotinic acid mononucleotide formation in mutants enzymes Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.4.21 0.3
-
 nicotinate
-
 Salmonella enterica subsp. enterica serovar Typhimurium
6.3.4.21 0.33 0.4 nicotinate mutant enzymes Salmonella enterica subsp. enterica serovar Typhimurium
6.3.4.21 2.3 48 5-phospho-alpha-D-ribose 1-diphosphate mutant enzymes Salmonella enterica subsp. enterica serovar Typhimurium
6.3.4.21 4.5
-
 5-phospho-alpha-D-ribose 1-diphosphate
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.4.21 45500
-
 mutant enzyme, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium
6.3.4.21 45529
-
 1 * 45529, gel filtration Salmonella enterica subsp. enterica serovar Typhimurium
6.3.4.21 45540
-
 SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium
6.3.4.21 45550
-
 mutant enzyme, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.21 Saccharomyces cerevisiae P39683
-
-
6.3.4.21 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Storage Stability

EC Number Storage Stability Organism
6.3.4.21 4ºC, as 70% saturated ammonium sulfate suspensions in 200 mM phosphate buffer, pH 8, 10% glycerol and 5 mM DTT Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.21 additional information
-
 Saccharomyces cerevisiae ?
-
 ?
6.3.4.21 additional information negligible ATPase activity in the absence of nicotinic acid Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?
6.3.4.21 nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
-
 Saccharomyces cerevisiae beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
 ?
6.3.4.21 nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O the enzyme is a facultative ATPase that uses ATP hydrolysis to drive the synthesis of nicotinate mononucleotide and diphosphate from nicotinic acid and phosphoribosyl diphosphate in a steady-state reaction, the enzyme undergoes phosphorylation in His-219 by bound ATP, phosphorylated enzyme has higher affinity for substrates than does nonphosphorylated enzyme Salmonella enterica subsp. enterica serovar Typhimurium beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
 ?
6.3.4.21 nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O mutants in the His-219 residue do catalyze the slow formation of nicotinic acid mononucleotide in the absence of ATP similarly to the wild type enzymes Salmonella enterica subsp. enterica serovar Typhimurium beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.4.21 monomer 1 * 45529, gel filtration Salmonella enterica subsp. enterica serovar Typhimurium

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.4.21 ATP 1 mol ATP is hydrolyzed to ADP plus phosphate per mol product formed Salmonella enterica subsp. enterica serovar Typhimurium
6.3.4.21 ATP 1 mol ATP is hydrolyzed to ADP plus phosphate per mol product formed Saccharomyces cerevisiae
6.3.4.21 ATP the Vmax of nicotinic acid mononucleotide synthesis activity is stimulated 10fold Salmonella enterica subsp. enterica serovar Typhimurium