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Literature summary extracted from

  • Chandrasekaran, E.V.; Chawda, R.; Rhodes, J.M.; Xia, J.; Piskorz, C.; Matta, K.L.
    Human lung adenocarcinoma alpha1,3/4-L-fucosyltransferase displays two molecular forms, high substrate affinity for clustered sialyl LacNAc type 1 units as well as mucin core 2 sialyl LacNAc type 2 unit and novel alpha1,2-L-fucosylating activity (2001), Glycobiology, 11, 353-363.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.65 NEM
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.65 0.045
-
 ancrod enzyme form FTB Homo sapiens
2.4.1.65 0.047
-
 3-O-sulfoGalbeta(1,3)GlcNAcbeta-O-Al enzyme form FTB Homo sapiens
2.4.1.65 0.1
-
 3-O-sulfoGalbeta(1,3)GlcNAcbeta-O-Al enzyme form FTA Homo sapiens
2.4.1.65 0.16
-
 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn enzyme form FTB Homo sapiens
2.4.1.65 0.167
-
 asialo ancrod enzyme form FTB Homo sapiens
2.4.1.65 0.4
-
 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn enzyme form FTA Homo sapiens
2.4.1.65 0.63
-
 fetuin triantennary asialoglycopeptide enzyme form FTB Homo sapiens
2.4.1.65 0.67
-
 NeuAcalpha(2,3)Galbeta(1,3)GlcNAcbeta-O-Bn enzyme form FTB Homo sapiens
2.4.1.65 0.77
-
 NeuAcalpha(2,3)Galbeta(1,4)GlcNAcbeta(1,6)(Galbeta(1,3))GalNAcalpha-OMe enzyme form FTB Homo sapiens
2.4.1.65 1.43
-
 fetuin triantennary glycopeptide enzyme form FTB Homo sapiens
2.4.1.65 1.52
-
 Fucalpha(1,2)Galbeta(1,4)Glc enzyme form FTB Homo sapiens
2.4.1.65 2.5
-
 NeuAcalpha(2,3)Galbeta(1,3)GlcNAcbeta-O-Bn enzyme form FTA Homo sapiens
2.4.1.65 3.3
-
 NeuAcalpha(2,3)Galbeta(1,4)GlcNAcbeta(1,6)(Galbeta(1,3))GalNAcalpha-OMe enzyme form FTB Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.65 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.65 two molecular forms: FTA and FTB Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.65 lung cancer cell
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.65 0.061
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.65 GDP-beta-L-fucose + fetuin triantennary asialoglycopeptide
-
 Homo sapiens ?
-
 ?
2.4.1.65 GDP-L-fucose + 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn
-
 Homo sapiens GDP + 2-O-MeGalbeta(1,3)(Fucalpha(1,4))GlcNAcbeta-O-Bn
-
 ?
2.4.1.65 GDP-L-fucose + 3-O-MeGalbeta(1,4)GlcNAcbeta(1,6)(Galbeta(1,3))GalNAcalpha-O-Bn 16.3% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTA. 17.8% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTB Homo sapiens GDP + 3-O-MeGalbeta(1,4)(Fucalpha(1,3))GlcNAcbeta(1,6)(Galbeta(1,3))GalNAcalpha-O-Bn
-
 ?
2.4.1.65 GDP-L-fucose + 3-O-sulfoGalbeta(1,3)GlcNAcbeta(1,3)Galbeta-O-Al 64.3% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTA. 82.2% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTB Homo sapiens GDP + 3-O-sulfoGalbeta(1,3)(Fucalpha(1,4))GlcNAcbeta(1,3)Galbeta-O-Al
-
 ?
2.4.1.65 GDP-L-fucose + 3-O-sulfoGalbeta(1,3)GlcNAcbeta-O-Al 64.3% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTA. 62.9% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTB Homo sapiens GDP + 3-O-sulfoGalbeta(1,3)(Fucalpha(1,4))GlcNAcbeta-O-Al
-
 ?
2.4.1.65 GDP-L-fucose + 3-O-sulfoGalbeta(1,4)GlcNAcbeta(1,6)(Galbeta(1,3))GalNAcalpha-O-Bn 10.9% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTA. 14.0% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTB Homo sapiens GDP + 3-O-sulfoGalbeta(1,4)(Fucalpha(1,3))GlcNAcbeta(1,6)(Galbeta(1,3))GalNAcalpha-O-Bn
-
 ?
2.4.1.65 GDP-L-fucose + 6-O-sulfoGalbeta(1,3)GlcNAcbeta-O-Al 3.1% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTA. 3.5% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTB Homo sapiens GDP + 6-O-sulfoGalbeta(1,3)(Fucalpha(1,4))GlcNAcbeta-O-Al
-
 ?
2.4.1.65 GDP-L-fucose + ancrod
-
 Homo sapiens GDP + ?
-
 ?
2.4.1.65 GDP-L-fucose + asialo ancrod
-
 Homo sapiens GDP + ?
-
 ?
2.4.1.65 GDP-L-fucose + fetuin triantennary glycopeptide
-
 Homo sapiens GDP + ?
-
 ?
2.4.1.65 GDP-L-fucose + Fucalpha(1,2)Galbeta(1,4)Glc 34.9% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTA. 38.7% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTB Homo sapiens GDP + Fucalpha(1,2)Galbeta(1,4)(Fucalpha(1,3))Glc
-
 ?
2.4.1.65 GDP-L-fucose + Galalpha(1,3)Galbeta(1,3)GlcNAcbeta-O-Naph 42.6 of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTA. 46.0% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTB Homo sapiens GDP + Galalpha(1,3)Galbeta(1,3)(Fucalpha(1,4))GlcNAcbeta-O-Naph
-
 ?
2.4.1.65 GDP-L-fucose + Galbeta(1,3)(6-O-sulfo)GlcNAcbeta(1,3)Galbeta-O-Al 15.5% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTA. 19.4% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTB Homo sapiens GDP + ?
-
 ?
2.4.1.65 GDP-L-fucose + Galbeta(1,3)GlcNAc 45.0% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTA. 51% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTB Homo sapiens GDP + Galbeta(1,3)(Fucalpha(1,4))GlcNAc
-
 ?
2.4.1.65 GDP-L-fucose + Galbeta(1,3)GlcNAcbeta-O-Bn 79.1% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTA. 83.8% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTB Homo sapiens GDP + Galbeta(1,3)(Fucalpha(1,4))GlcNAcbeta-O-Bn
-
 ?
2.4.1.65 GDP-L-fucose + Galbeta(1,4)GlcNAc 3.9% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTA. 3.5% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTB Homo sapiens GDP + Galbeta(1,4)(Fucalpha(1,3))GlcNAc
-
 ?
2.4.1.65 GDP-L-fucose + Galbeta(1,4)GlcNAcbeta(1,6)(3-O-MeGalbeta(1,3))GalNAcalpha-O-Bn 2.3% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTA. 1.9% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTB Homo sapiens GDP + Galbeta(1,4)(Fucalpha(1,3))GlcNAcbeta(1,6)(3-O-MeGalbeta(1,3))GalNAcalpha-O-Bn
-
 ?
2.4.1.65 GDP-L-fucose + Galbeta(1,4)GlcNAcbeta(1,6)(3-O-sulfoGalbeta(1,3))-GalNAcalpha-O-Bn 1.6% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTA. 1.6% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTB Homo sapiens GDP + Galbeta(1,4)(Fucalpha(1,3))GlcNAcbeta(1,6)(3-O-sulfoGalbeta(1,3))-GalNAcalpha-O-Bn
-
 ?
2.4.1.65 GDP-L-fucose + Galbeta(1,4)GlcNAcbeta(1,6)(Galbeta(1,3))GalNAcalpha-O-Bn 2.3% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTA. 1.6% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTB Homo sapiens GDP + Galbeta(1,4)(Fucalpha(1,3))GlcNAcbeta(1,6)(Galbeta(1,3))GalNAcalpha-O-Bn
-
 ?
2.4.1.65 GDP-L-fucose + Galbeta(1,4)GlcNAcbeta-O-Bn 3.1% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTA. 2.5% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTB Homo sapiens GDP + Galbeta(1,4)(Fucalpha(1,3))GlcNAcbeta-O-Bn
-
 ?
2.4.1.65 GDP-L-fucose + GalNAcbeta(1,3)(6-O-sulfo)GlcNAcbeta-O-Me 6.2 of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTA. 8.6% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTB Homo sapiens GDP + ?
-
 ?
2.4.1.65 GDP-L-fucose + NeuAcalpha(2,3)Galbeta(1,3)GlcNAcbeta-O-Bn 55.8% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTA. 64.4% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTB Homo sapiens GDP + NeuAcalpha(2,3)Galbeta(1,3)(Fucalpha(1,4))GlcNAcbeta-O-Bn
-
 ?
2.4.1.65 GDP-L-fucose + NeuAcalpha(2,3)Galbeta(1,4)GlcNAcbeta(1,6)(Galbeta(1,3))GalNAcalpha-OMe 24.2% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTA. 12.9% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTB Homo sapiens GDP + NeuAcalpha(2,3)Galbeta(1,4)(Fucalpha(1,3))GlcNAcbeta(1,6)(Galbeta(1,3))GalNAcalpha-OMe
-
 ?
2.4.1.65 GDP-L-fucose + NeuAcalpha(2,3)Galbeta(1,4)GlcNAcbeta-O-Bn 6.2% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTA. 7.6% of the activity with 2-O-MeGalbeta(1,3)GlcNAcbeta-O-Bn, enzyme form FTB Homo sapiens GDP + NeuAcalpha(2,3)Galbeta(1,4)(Fucalpha(1,3))GlcNAcbeta-O-Bn
-
 ?
2.4.1.65 additional information high substrate affinity for clustered units of 3-sialyl Galbeta(1,3)GlcNAcbeta in asparagine linked carbohydrate as well as for mucin core 2 structure containing 3-sialyl Galbeta1,4GlcNAcbeta-unit, in addition of alpha(1,2)-L-fucosylating activity Homo sapiens ?
-
 ?

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.1.65 0.25
-
 NEM enzyme form FTA and FTB Homo sapiens