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Literature summary extracted from

  • Marquardt, J.L.; Siegele, D.A.; Kolter, R.; Walsh, C.T.
    Cloning and sequencing of Escherichia coli murZ and purification of its product, a UDP-N-acetylglucosamine enolpyruvyl transferase (1992), J. Bacteriol., 174, 5748-5752.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.7 DNA and amino acid sequence determination and analysis, chromosome mapping at 69.3 min, overexpression in Escherichia coli strain JLM 16 Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.7 phosphonomycin irreversible inactivation, requires the presence of UDP-GlcNAc Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.7 1
-
 phosphoenolpyruvate
-
 Escherichia coli
2.5.1.7 2.5
-
 UDP-GlcNAc
-
 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.7 44800
-
 MW deduced from amino acid sequence Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.7 Escherichia coli P0A749 gene murZ, at 69.3 min of the chromosome
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.7 native and recombinant from Escherichia coli Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.7 phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine
-
 Escherichia coli phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.7 4.75
-
 phosphoenolpyruvate recombinant enzyme Escherichia coli
2.5.1.7 4.75
-
 UDP-N-acetyl-D-glucosamine recombinant enzyme Escherichia coli
2.5.1.7 4.75
-
 phosphoenolpyruvate cosubstrate UDP-N-acetyl-D-glucosamine Escherichia coli
2.5.1.7 4.75
-
 UDP-N-acetyl-D-glucosamine cosubstrate phosphoenolpyruvate Escherichia coli