Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Della Ragione, F.; Takabayashi, K.; Mastropietro, S.; Mercurio, C.; Oliva, A.; Russo, G.L.; Della Pietra, V.; Borriello, A.; Nobori, T.; et al.
    Purification and characterization of recombinant human 5'-methylthioadenosine phosphorylase: definite identification coding cDNA (1996), Biochem. Biophys. Res. Commun., 223, 514-519.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.4.2.28 medicine gene is linked to the important tumor suppressor gene p16INK4A, enzyme is of key importance in defining the chromosomal area homozygously deleted in a large number of human tumors, absence of enzyme activity only in malignant cells is of great value in developing selective antitumor therapeutic strategies Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.28 the gene maps on the 9p21 chromosome, strictly linked to the tumor suppressor gene p16INK4A, functional expression in Escherichia coli strain JM105 Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.28 5'-Chloroformycin
-
 Homo sapiens
2.4.2.28 5'-methylthiotubercidin
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.28 0.004
-
 5'-methylthioadenosine recombinant enzyme Homo sapiens
2.4.2.28 0.75
-
 phosphate recombinant enzyme Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.28 30000
-
 3 * 30000, recombinant enzyme, SDS-PAGE Homo sapiens
2.4.2.28 90000
-
 recombinant enzyme, gel filtration Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.28 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.28 recombinant from Escherichia coli Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.28 9.5
-
 purified recombinant enzyme Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.28 5'-methylthioadenosine + phosphate
-
 Homo sapiens adenine + 5-methylthio-D-ribose 1-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.28 trimer 3 * 30000, recombinant enzyme, SDS-PAGE Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.28 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.28 7.4
-
 assay at Homo sapiens