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Literature summary extracted from

  • Chang, S.Y.; Tsai, P.C.; Tseng, C.S.; Liang, P.H.
    Refolding and characterization of a yeast dehydrodolichyl diphosphate synthase overexpressed in Escherichia coli (2001), Protein Expr. Purif., 23, 432-439.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.31 expression in Escherichia coli. Thioredoxin and His tag are utilized to increase the solubility of the recombinant protein and facilitate its purification using Ni-nitrilotriacetic acid column Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.31 0.027
-
 isopentenyl diphosphate
-
 Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.31 additional information the recombinant enzyme expressed in Escherichia coli mostly exists in pellet in the absence of detergents, a low quantity of soluble enzyme is purified Saccharomyces cerevisiae
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.31 additional information Saccharomyces cerevisiae the synthesized long-chain dehydrodolichyl diphosphate serves as a precursor of glycosyl carrier in glycoprotein biosynthesis in eukaryotes ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.31 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.31
-
 Saccharomyces cerevisiae

Renatured (Commentary)

EC Number Renatured (Comment) Organism
2.5.1.31 the recombinant protein in the pellet is solubilized with 7 M urea and purified using nickel-nitrilotriacetic acid under denaturing conditions. The protein refolding is achieved via the stepwise dialysis to remove the denaturant in the presence of 6 mM beta-mercaptoethanol. Alternatively, on-column refolding is carried out in a single step to obtain the active protein in large quantities. beta-Mercaptoethanol and Triton are both required in this quick refolding process Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.31 isopentenyl diphosphate + farnesyl diphosphate
-
 Saccharomyces cerevisiae C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate the C55 polymer is the major product in presence of Triton X-100, without Triton C55-C75 polyprenyl diphosphates are generated ?
2.5.1.31 additional information the synthesized long-chain dehydrodolichyl diphosphate serves as a precursor of glycosyl carrier in glycoprotein biosynthesis in eukaryotes Saccharomyces cerevisiae ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.31 additional information
-
 additional information
-
 Saccharomyces cerevisiae
2.5.1.31 0.0002
-
 isopentenyl diphosphate
-
 Saccharomyces cerevisiae