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Literature summary extracted from

  • Hemmi, H.; Ikejiri, S.; Yamashita, S.; Nishino, T.
    Novel medium-chain prenyl diphosphate synthase from the thermoacidophilic archaeon Sulfolobus solfataricus (2002), J. Bacteriol., 184, 615-620.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.5.1.82 Triton X-100 approximately increases the activity of the enzyme 2fold and retains the effect at a similar level through various concentrations from 0.01 to 1% Saccharolobus solfataricus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.82 expression in Escherichia coli Saccharolobus solfataricus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.82 Mg2+ the enzyme does not show activity without a divalent cation and prefers Mg2+ to other cations such as Mn2+ and Ca2+. At concentrations ranging from 2.5 to 50 mM, the effect of Mg2+ does not change greatly Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.82 32000
-
 x * 32000, SDS-PAGE Saccharolobus solfataricus
2.5.1.82 32274
-
 x * 32274, calculated from sequence Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.82 geranylgeranyl diphosphate + 2 isopentenyl diphosphate Saccharolobus solfataricus
-
 2 diphosphate + all-trans-hexaprenyl diphosphate
-
 ?
2.5.1.82 geranylgeranyl diphosphate + 2 isopentenyl diphosphate Saccharolobus solfataricus P2
-
 2 diphosphate + all-trans-hexaprenyl diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.82 Saccharolobus solfataricus Q97W92
-
-
2.5.1.82 Saccharolobus solfataricus P2 Q97W92
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.82 recombinant enzyme Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.82 farnesyl diphosphate + 3 isopentenyl diphosphate the enzyme prefers geranylgeranyl diphosphate to farnesyl diphosphate as an allylic substrate and does not show activity for geranyl diphosphate and dimethylallyl diphosphate Saccharolobus solfataricus 3 diphosphate + all-trans-hexaprenyl diphosphate
-
 ?
2.5.1.82 farnesyl diphosphate + 3 isopentenyl diphosphate the enzyme prefers geranylgeranyl diphosphate to farnesyl diphosphate as an allylic substrate and does not show activity for geranyl diphosphate and dimethylallyl diphosphate Saccharolobus solfataricus P2 3 diphosphate + all-trans-hexaprenyl diphosphate
-
 ?
2.5.1.82 geranylgeranyl diphosphate + 2 isopentenyl diphosphate
-
 Saccharolobus solfataricus 2 diphosphate + all-trans-hexaprenyl diphosphate
-
 ?
2.5.1.82 geranylgeranyl diphosphate + 2 isopentenyl diphosphate the enzyme prefers geranylgeranyl diphosphate to farnesyl diphosphate as an allylic substrate and does not show activity for geranyl diphosphate and dimethylallyl diphosphate. The main product is hexaprenyl diphosphate (C30) when the concentrations of isopentenyl diphosphate and geranylgeranyl diphosphate are the same. A 10-fold increase in the concentration of isopentenyl diphosphate results in the increased chain length of the products: a significant amount of heptaprenyl diphosphate (C35) is produced, although hexaprenyl diphosphate remains the main product Saccharolobus solfataricus 2 diphosphate + all-trans-hexaprenyl diphosphate
-
 ?
2.5.1.82 geranylgeranyl diphosphate + 2 isopentenyl diphosphate
-
 Saccharolobus solfataricus P2 2 diphosphate + all-trans-hexaprenyl diphosphate
-
 ?
2.5.1.82 geranylgeranyl diphosphate + 2 isopentenyl diphosphate the enzyme prefers geranylgeranyl diphosphate to farnesyl diphosphate as an allylic substrate and does not show activity for geranyl diphosphate and dimethylallyl diphosphate. The main product is hexaprenyl diphosphate (C30) when the concentrations of isopentenyl diphosphate and geranylgeranyl diphosphate are the same. A 10-fold increase in the concentration of isopentenyl diphosphate results in the increased chain length of the products: a significant amount of heptaprenyl diphosphate (C35) is produced, although hexaprenyl diphosphate remains the main product Saccharolobus solfataricus P2 2 diphosphate + all-trans-hexaprenyl diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.82 ? x * 32000, SDS-PAGE Saccharolobus solfataricus
2.5.1.82 ? x * 32274, calculated from sequence Saccharolobus solfataricus

Synonyms

EC Number Synonyms Comment Organism
2.5.1.82 (all-E) hexaprenyl diphosphate synthase
-
 Saccharolobus solfataricus
2.5.1.82 (all-trans) hexaprenyl diphosphate synthase
-
 Saccharolobus solfataricus
2.5.1.82 HexPS
-
 Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.82 6
-
-
 Saccharolobus solfataricus