Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Cress, W.A.; Chayet, L.T.; Rilling, H.C.
    Crystallization and partial characterization of dimethylallyl pyrophosphate: L-tryptophan dimethylallyltransferase from Claviceps sp. SD58 (1981), J. Biol. Chem., 256, 10917-10923.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.5.1.34
-
 Claviceps sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.34 1,10-phenanthroline
-
 Claviceps sp.
2.5.1.34 2,2'-bipyridyl
-
 Claviceps sp.
2.5.1.34 8-hydroxyquinoline
-
 Claviceps sp.
2.5.1.34 8-mercaptoquinoline
-
 Claviceps sp.
2.5.1.34 Acetylacetone
-
 Claviceps sp.
2.5.1.34 Chelating agents in presence of excess of Ca2+ Claviceps sp.
2.5.1.34 dithiothreitol
-
 Claviceps sp.
2.5.1.34 EDTA
-
 Claviceps sp.
2.5.1.34 nitrilotriacetic acid
-
 Claviceps sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.34 additional information metal ion, free in solution not required for activity Claviceps sp.
2.5.1.34 additional information enzyme contains a divalent cation which is most likely involved in catalysis Claviceps sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.34 34000
-
 2 * 34000, SDS-PAGE Claviceps sp.
2.5.1.34 70000
-
 gel filtration Claviceps sp.

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.34 Claviceps sp.
-
 SD58
-
2.5.1.34 Claviceps sp. SD58
-
 SD58
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.34
-
 Claviceps sp.

Reaction

EC Number Reaction Comment Organism Reaction ID
2.5.1.34 dimethylallyl diphosphate + L-tryptophan = diphosphate + 4-(3-methylbut-2-enyl)-L-tryptophan random sequential mechanism Claviceps sp.
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.34 0.35
-
-
 Claviceps sp.

Storage Stability

EC Number Storage Stability Organism
2.5.1.34 -20°C, 50% loss of activity after several weeks Claviceps sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.34 dimethylallyl diphosphate + L-tryptophan
-
 Claviceps sp. diphosphate + L-4-(gamma,gamma-dimethylallyl)tryptophan
-
 ?
2.5.1.34 dimethylallyl diphosphate + L-tryptophan
-
 Claviceps sp. SD58 diphosphate + L-4-(gamma,gamma-dimethylallyl)tryptophan
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.34 dimer 2 * 34000, SDS-PAGE Claviceps sp.