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Literature summary extracted from

  • Clausen, T.; Wahl, M.C.; Messerschmidt, A.; Huber, R.; Fuhrmann, J.C.; Laber, B.; Streber, W.; Steegborn, C.
    Cloning, purification, and characterization of cystathionine g-synthase from Nicotiana tabacum (1999), Biol. Chem., 380, 1237-1242.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.48 overexpression in Escherichia coli Nicotiana tabacum

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.5.1.48
-
 Nicotiana tabacum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.48 (Z)-3-(2-phosphonoethen-1-yl)pyridine-2-carboxylic acid
-
 Nicotiana tabacum
2.5.1.48 3-(phosphonomethyl)pyridine-2-carboxylic acid
-
 Nicotiana tabacum
2.5.1.48 4-(phosphonomethyl)pyridine-2-carboxylic acid
-
 Nicotiana tabacum
2.5.1.48 DL-(E)-2-amino-5-phosphono-3-pentenoic acid
-
 Nicotiana tabacum
2.5.1.48 DL-propargylglycine irreversible Nicotiana tabacum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.48 0.23
-
 L-cysteine
-
 Nicotiana tabacum
2.5.1.48 0.55
-
 Sodium sulfide
-
 Nicotiana tabacum
2.5.1.48 0.89
-
 L-cysteine methyl ester
-
 Nicotiana tabacum
2.5.1.48 2.6
-
 2-mercaptoethanol
-
 Nicotiana tabacum
2.5.1.48 7.1
-
 O-phospho-L-homoserine
-
 Nicotiana tabacum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.48 O-succinyl-L-homoserine + L-cysteine Nicotiana tabacum the enzyme catalyzes the first reaction specific for methionine biosynthesis L-cystathionine + succinate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.48 Nicotiana tabacum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.48 recombinant enzyme Nicotiana tabacum

Reaction

EC Number Reaction Comment Organism Reaction ID
2.5.1.48 O4-succinyl-L-homoserine + L-cysteine = L-cystathionine + succinate ping-pong mechanism Nicotiana tabacum
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.48 O-acetyl-L-homoserine + L-Cys methyl ester 26% of the activity with L-Cys Nicotiana tabacum S-(L-2-amino-2-methoxycarbonyl)-L-homocysteine + acetate
-
 ?
2.5.1.48 O-phospho-L-homoserine + L-cysteine
-
 Nicotiana tabacum L-cystathionine + phosphate
-
 ?
2.5.1.48 O-succinyl-L-homoserine + 2-mercaptoethanol 32% of the activity with L-cysteine Nicotiana tabacum S-ethyl-L-homocysteine + succinate
-
 ?
2.5.1.48 O-succinyl-L-homoserine + D-cysteine 20% of the activity with L-cysteine Nicotiana tabacum ?
-
 ?
2.5.1.48 O-succinyl-L-homoserine + L-cysteine the enzyme catalyzes the first reaction specific for methionine biosynthesis Nicotiana tabacum L-cystathionine + succinate
-
 ?
2.5.1.48 O-succinyl-L-homoserine + sodium sulfide 62% of the activity with L-cysteine Nicotiana tabacum L-homocysteine + sodium succinate
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
2.5.1.48 pyridoxal 5'-phosphate enzyme is dependent on Nicotiana tabacum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.48 0.018
-
 DL-propargylglycine
-
 Nicotiana tabacum
2.5.1.48 0.027
-
 DL-(E)-2-amino-5-phosphono-3-pentenoic acid
-
 Nicotiana tabacum
2.5.1.48 0.2
-
 3-(phosphonomethyl)pyridine-2-carboxylic acid
-
 Nicotiana tabacum
2.5.1.48 0.3
-
 4-(phosphonomethyl)pyridine-2-carboxylic acid
-
 Nicotiana tabacum
2.5.1.48 0.45
-
 (Z)-3-(2-phosphonoethen-1-yl)pyridine-2-carboxylic acid
-
 Nicotiana tabacum