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Literature summary extracted from

  • Ravanel, S.; Droux, M.; Douce, R.
    Methionine biosynthesis in higher plants. I. Purification and characterization of cystathionine gamma-synthase from spinach chloroplasts (1995), Arch. Biochem. Biophys., 316, 572-584.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.48 propargylglycine
-
 Spinacia oleracea
2.5.1.160 additional information not inhibited by 5 mM L-alpha-(2-aminoethoxyvinyl)glycine Spinacia oleracea
2.5.1.160 propargylglycine reversible inhibition Spinacia oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.48 0.18
-
 L-cysteine pH 7.5, 30°C Spinacia oleracea
2.5.1.48 0.6
-
 S2- pH 7.5, 30°C Spinacia oleracea
2.5.1.48 1.4
-
 O-phospho-L-homoserine pH 7.5, 30°C Spinacia oleracea
2.5.1.48 13.34
-
 O-succinyl-L-homoserine pH 7.5, 30°C Spinacia oleracea
2.5.1.160 0.18
-
 L-cysteine at pH 7.5 and 30°C Spinacia oleracea
2.5.1.160 1.4
-
 O-phospho-L-homoserine at pH 7.5 and 30°C Spinacia oleracea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.48 chloroplast
-
 Spinacia oleracea 9507
-
2.5.1.160 chloroplast
-
 Spinacia oleracea 9507
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.48 50000
-
 4 * 50000 or 2 * 50000 + 2 * 53000, SDS-PAGE Spinacia oleracea
2.5.1.48 53000
-
 4 * 50000 or 2 * 50000 + 2 * 53000, SDS-PAGE Spinacia oleracea
2.5.1.48 215000
-
 gel filtration Spinacia oleracea
2.5.1.160 215000
-
 gel filtration Spinacia oleracea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.160 O-phospho-L-homoserine + L-cysteine Spinacia oleracea
-
 L-cystathionine + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.48 Spinacia oleracea
-
 spinach
-
2.5.1.160 Spinacia oleracea
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.5.1.48 proteolytic modification the 50000 Da polypeptide may be derived from the 53000 Da polypeptide by proteolysis Spinacia oleracea

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.48
-
 Spinacia oleracea
2.5.1.160 heat treatment, ammonium sulfate precipitation, Fractogel EMD DEAE 650(M) column chromatography, phenyl Sepharose column chromatography, Accekk Plus QMA chromatography, Superdex 200 gel filtration, and Mono Q column chromatography Spinacia oleracea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.48 leaf
-
 Spinacia oleracea
-
2.5.1.160 leaf
-
 Spinacia oleracea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.48 13.11
-
-
 Spinacia oleracea

Storage Stability

EC Number Storage Stability Organism
2.5.1.48 -20°C, 20 mM MOPS-NaOH, pH 7.5, 1 mM EDTA, 1 mM DTT, 10% v/v glycerol, 5 days, 50% loss of activity Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.48 L-cystathionine + H2O
-
 Spinacia oleracea L-homocysteine + NH3 + pyruvate
-
 ?
2.5.1.48 O-phospho-L-homoserine + L-cysteine
-
 Spinacia oleracea L-cystathionine + phosphate
-
 ?
2.5.1.48 O-phospho-L-homoserine + S2-
-
 Spinacia oleracea L-homocysteine + phosphate
-
 ?
2.5.1.48 O-succinyl-L-homoserine + L-cysteine
-
 Spinacia oleracea L-cystathionine + succinate
-
 ?
2.5.1.160 O-phospho-L-homoserine + L-cysteine
-
 Spinacia oleracea L-cystathionine + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.48 tetramer 4 * 50000 or 2 * 50000 + 2 * 53000, SDS-PAGE Spinacia oleracea
2.5.1.160 tetramer 4 * 53000, SDS-PAGE Spinacia oleracea

Synonyms

EC Number Synonyms Comment Organism
2.5.1.160 CGS
-
 Spinacia oleracea
2.5.1.160 cystathionine gamma-synthase
-
 Spinacia oleracea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.48 7.4
-
-
 Spinacia oleracea
2.5.1.160 7.4
-
-
 Spinacia oleracea

Cofactor

EC Number Cofactor Comment Organism Structure
2.5.1.160 pyridoxal 5'-phosphate dependent on Spinacia oleracea

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.160 0.14
-
 propargylglycine at pH 7.5 and 20°C Spinacia oleracea