Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Duewel, H.S.; Sheflyan, G.Y.; Woodard, R.W.
    Functional and biochemical characterization of a recombinant 3-deoxy-D-manno-octulosonic acid 8-phosphate synthase from the hyperthermophilic bacterium Aquifex aeolicus (1999), Biochem. Biophys. Res. Commun., 263, 346-351.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.55 expression in Escherichia coli Aquifex aeolicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.55 0.008
-
 D-arabinose 5-phosphate pH 7.5, 60°C Aquifex aeolicus
2.5.1.55 0.015
-
 D-arabinose 5-phosphate pH 7.5, 70°C Aquifex aeolicus
2.5.1.55 0.027
-
 D-arabinose 5-phosphate pH 7.5, 80°C Aquifex aeolicus
2.5.1.55 0.028
-
 phosphoenolpyruvate pH 7.5, at 90°C Aquifex aeolicus
2.5.1.55 0.038
-
 phosphoenolpyruvate pH 7.5, at 80°C Aquifex aeolicus
2.5.1.55 0.043
-
 phosphoenolpyruvate pH 7.5, at 60°C or 70°C Aquifex aeolicus
2.5.1.55 0.074
-
 D-arabinose 5-phosphate pH 7.5, 90°C Aquifex aeolicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.55 29736
-
 3 * 29736, electrospray ionization mass spectrometry Aquifex aeolicus
2.5.1.55 97600
-
 gel filtration Aquifex aeolicus

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.55 Aquifex aeolicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.55
-
 Aquifex aeolicus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.55 3.38
-
-
 Aquifex aeolicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.55 phosphoenolpyruvate + D-arabinose 5-phosphate
-
 Aquifex aeolicus 2-dehydro-3-deoxy-D-octonate 8-phosphate + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.55 ? 3 * 29736, electrospray ionization mass spectrometry Aquifex aeolicus
2.5.1.55 trimer
-
 Aquifex aeolicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.55 95
-
-
 Aquifex aeolicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.55 70
-
 half-life: 30.3 h Aquifex aeolicus
2.5.1.55 80
-
 half-life: 8.1 h Aquifex aeolicus
2.5.1.55 90
-
 half-life: 1.5 h Aquifex aeolicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.55 0.38
-
 phosphoenolpyruvate pH 7.5, 60°C Aquifex aeolicus
2.5.1.55 0.38
-
 D-arabinose 5-phosphate pH 7.5, 60°C Aquifex aeolicus
2.5.1.55 0.87
-
 phosphoenolpyruvate pH 7.5, 70°C Aquifex aeolicus
2.5.1.55 0.87
-
 D-arabinose 5-phosphate pH 7.5, 70°C Aquifex aeolicus
2.5.1.55 1.47
-
 phosphoenolpyruvate pH 7.5, 80°C Aquifex aeolicus
2.5.1.55 1.47
-
 D-arabinose 5-phosphate pH 7.5, 80°C Aquifex aeolicus
2.5.1.55 2
-
 phosphoenolpyruvate pH 7.5, 90°C Aquifex aeolicus
2.5.1.55 2
-
 D-arabinose 5-phosphate pH 7.5, 90°C Aquifex aeolicus