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Literature summary extracted from

  • Yamagata, S.
    O-Acetylserine and O-acetylhomoserine sulfhydrylase of yeast. Subunit structure (1976), J. Biochem., 80, 787-797.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.5.1.49 dithiothreitol 2 mM, catalytic activity 124% Saccharomyces cerevisiae
2.5.1.49 ferricyanide 1 mM, catalytic activity 102-119% Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.47 51000
-
 4 * 51000, SDS-PAGE, association does not require disulfide linkage Saccharomyces cerevisiae
2.5.1.47 200000
-
 gel filtration Saccharomyces cerevisiae
2.5.1.49 51000
-
 4 * 51000, SDS-PAGE Saccharomyces cerevisiae
2.5.1.49 57000
-
 4 * 57000, gel filtration in presence of urea and beta-mercaptoethanol Saccharomyces cerevisiae
2.5.1.49 200000
-
 gel filtration, sucrose density gradient centrifugation Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.47 Saccharomyces cerevisiae
-
-
-
2.5.1.49 Saccharomyces cerevisiae
-
 baker's yeast
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.49
-
 Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.49 30
-
-
 Saccharomyces cerevisiae

Subunits

EC Number Subunits Comment Organism
2.5.1.47 More amino acid analysis, one sulfhydryl group per subunit, essential for restoration of native subunit structure Saccharomyces cerevisiae
2.5.1.47 tetramer 4 * 51000, SDS-PAGE, association does not require disulfide linkage Saccharomyces cerevisiae
2.5.1.49 tetramer 4 * 51000, SDS-PAGE Saccharomyces cerevisiae
2.5.1.49 tetramer 4 * 57000, gel filtration in presence of urea and beta-mercaptoethanol Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
2.5.1.49 pyridoxal 5'-phosphate
-
 Saccharomyces cerevisiae