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Literature summary extracted from

  • Schaffrath, C.; Deng, H.; O’Hagan, D.
    Isolation and characterisation of 5’-fluorodeoxyadenosine synthase, a fluorination enzyme from Streptomyces cattleya (2003), FEBS Lett., 547, 111-114.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.5.1.63 EDTA 1 mM increases enzyme activity by 25% Streptomyces cattleya

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.63 S-adenosyl-L-homocysteine competent, competitive Streptomyces cattleya
2.5.1.63 sinefungin weak Streptomyces cattleya

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.63 0.42
-
 S-adenosyl-L-methionine
-
 Streptomyces cattleya
2.5.1.63 8.56
-
 fluoride
-
 Streptomyces cattleya

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.63 additional information Mg2+ at 1 mM does not affect enzyme activity Streptomyces cattleya

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.63 32000
-
 6 * 32000, SDS-PAGE and electrospray mass spectrometry Streptomyces cattleya
2.5.1.63 180000
-
 gel filtration Streptomyces cattleya

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.63 S-adenosyl-L-methionine + fluoride Streptomyces cattleya involved in production of toxic fluoroacetate 5'-deoxy-5'-fluoroadenosine + L-methionine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.63 Streptomyces cattleya
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.63
-
 Streptomyces cattleya

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.63 S-adenosyl-L-methionine + fluoride
-
 Streptomyces cattleya 5'-deoxy-5'-fluoroadenosine + L-methionine
-
 ?
2.5.1.63 S-adenosyl-L-methionine + fluoride involved in production of toxic fluoroacetate Streptomyces cattleya 5'-deoxy-5'-fluoroadenosine + L-methionine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.63 hexamer 6 * 32000, SDS-PAGE and electrospray mass spectrometry Streptomyces cattleya

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.63 55
-
-
 Streptomyces cattleya

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.63 0.029
-
 S-adenosyl-homocysteine
-
 Streptomyces cattleya