EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.1.34 | Thermus thermophilus | - |
- |
- |
2.1.1.34 | Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | - |
- |
- |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.1.1.34 | S-adenosyl-L-methionine + guanosine18 in tRNA = S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA | substrate recognition sites and mechanism | Thermus thermophilus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.34 | additional information | specifically methylates guanosine at position 18 in the D-loop of tRNAPhe from E. coli | Thermus thermophilus | ? | - |
? | |
2.1.1.34 | additional information | specifically methylates guanosine at position 18 in the D-loop of tRNAPhe from E. coli | Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | ? | - |
? | |
2.1.1.34 | S-adenosyl-L-methionine + guanosine18 in tRNA | methyl group acceptor from yeast: tRNAPhe | Thermus thermophilus | S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA | - |
? | |
2.1.1.34 | S-adenosyl-L-methionine + guanosine18 in tRNA | methyl group acceptors from E. coli: methyl deficient tRNATyr, tRNAPhe, tRNAfMet | Thermus thermophilus | S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA | - |
? | |
2.1.1.34 | S-adenosyl-L-methionine + guanosine18 in tRNA | methyl group acceptors from Bacillus subtilis: tRNAVal, tRNALys, tRNAThr, tRNALeu | Thermus thermophilus | S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA | - |
? | |
2.1.1.34 | S-adenosyl-L-methionine + guanosine18 in tRNA | methyl group acceptor from yeast: tRNAPhe | Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA | - |
? | |
2.1.1.34 | S-adenosyl-L-methionine + guanosine18 in tRNA | methyl group acceptors from E. coli: methyl deficient tRNATyr, tRNAPhe, tRNAfMet | Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA | - |
? | |
2.1.1.34 | S-adenosyl-L-methionine + guanosine18 in tRNA | methyl group acceptors from Bacillus subtilis: tRNAVal, tRNALys, tRNAThr, tRNALeu | Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.1.1.34 | additional information | - |
optimal temperature for methylation is ca. 20°C lower than the melting temperature of the tRNA, overview | Thermus thermophilus |
2.1.1.34 | 45 | - |
Bacillus subtilis tRNALeu | Thermus thermophilus |
2.1.1.34 | 55 | - |
Bacillus subtilis tRNAThr | Thermus thermophilus |
2.1.1.34 | 60 | - |
Bacillus subtilis tRNALys | Thermus thermophilus |
2.1.1.34 | 60 | - |
yeast tRNAPhe | Thermus thermophilus |
2.1.1.34 | 65 | - |
Bacillus subtilis tRNAVal | Thermus thermophilus |
2.1.1.34 | 65 | - |
Escherichia coli tRNAfMet | Thermus thermophilus |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.1.1.34 | 55 | 75 | about half-maximal activity at 55°C and 75°C, Escherichia coli tRNAfMet | Thermus thermophilus |
2.1.1.34 | 55 | 68 | about half-maximal activity at 55°C and 68°C, yeast tRNAPhe | Thermus thermophilus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.1.1.34 | 60 | - |
stable below, tRNAPhe | Thermus thermophilus |
2.1.1.34 | 70 | - |
stable below, tRNAMet | Thermus thermophilus |