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Literature summary extracted from

  • Eisenberg, M.A.; Stoner, G.L.
    7,8-Diaminopelargonic acid aminotransferase (1979), Methods Enzymol., 62, 342-347.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.62 7-amino-8-oxononanoate
-
 Escherichia coli
2.6.1.62 7-Keto-8-aminopelargonic acid competitive with adenosyl-L-methionine Escherichia coli
2.6.1.62 adenosine
-
 Escherichia coli
2.6.1.62 S-Adenosyl-L-(2-hydroxy-4-methylthio)butyric acid
-
 Escherichia coli
2.6.1.62 S-adenosyl-L-ethionine
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.62 0.0002
-
 S-adenosyl-L-methionine pH 8.5, 37°C Escherichia coli
2.6.1.62 0.001
-
 7-amino-8-oxononanoate pH 8.5, 37°C Escherichia coli
2.6.1.62 0.0012
-
 8-Amino-7-oxononanoate pH 8.5, 37°C Escherichia coli
2.6.1.62 0.021
-
 pyridoxamine 5'-phosphate pH 8.5, 37°C Escherichia coli
2.6.1.62 0.032
-
 pyridoxal 5'-phosphate pH 8.5, 37°C Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.62 47000
-
 2 * 47000, SDS-PAGE Escherichia coli
2.6.1.62 94000
-
 gel filtration, sucrose density gradient sedimentation Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.62 Escherichia coli
-
 regulatory mutant
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.62
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.62 98
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.6.1.62 -20°C, 20% glycerol, stable for 1 year Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.62 S-adenosyl-L-methionine + 7,8-diketopelargonic acid
-
 Escherichia coli S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate
-
 ?
2.6.1.62 S-adenosyl-L-methionine + 7-amino-8-oxononanoate
-
 Escherichia coli S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate S-adenosyl-4-methylthio-2-oxobutanoate may be decomposed nonenzymatically to form 5'-methylthioadenosine and 2-oxo-3-butenoic acid ?
2.6.1.62 S-adenosyl-L-methionine + 8-amino-7-oxononanoate synonym: 7-keto-8-aminopelargonic acid, KAPA Escherichia coli S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate S-adenosyl-4-methylthio-2-oxobutanoate may be decomposed nonenzymatically to form 5'-methylthioadenosine and 2-oxo-3-butenoic acid ?

Subunits

EC Number Subunits Comment Organism
2.6.1.62 dimer 2 * 47000, SDS-PAGE Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.6.1.62 0.283
-
 8-Amino-7-oxononanoate pH 8.5, 37°C Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.6.1.62 0.025
-
 7-Keto-8-aminopelargonic acid pH 8.5, 37°C Escherichia coli