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Literature summary extracted from

  • Soda, K.; Misono, H.
    L-Lysine:alpha-ketoglutarate aminotransferase. II. Purification, crystallization, and properties (1968), Biochemistry, 7, 4110-4119.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.6.1.36
-
 [Flavobacterium] lutescens

General Stability

EC Number General Stability Organism
2.6.1.36 pyridoxal 5'-phosphate, 0.1 mM, or 2-mercaptoethanol, 0.02%, protects against heat inactivation [Flavobacterium] lutescens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.36 additional information incubation of the enzyme with L-lysine in the presence of high concentrations of phosphate gives an inactive form of the enzyme (semiapoenzyme), reactivation with pyridoxal 5'-phosphate [Flavobacterium] lutescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.36 0.13
-
 2-oxoglutarate cosubstrate L-ornithine, pH 8.0, 37°C [Flavobacterium] lutescens
2.6.1.36 0.5
-
 2-oxoglutarate L-lysine, pH 8.0, 37°C [Flavobacterium] lutescens
2.6.1.36 2
-
 L-ornithine pH 8.0, 37°C [Flavobacterium] lutescens
2.6.1.36 2.8
-
 L-lysine pH 8.0, 37°C [Flavobacterium] lutescens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.36 116000
-
 sedimentation equilibrium method [Flavobacterium] lutescens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.36 L-lysine + 2-oxoglutarate [Flavobacterium] lutescens
-
 2-aminoadipate 6-semialdehyde + L-glutamate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.36 [Flavobacterium] lutescens
-
 i.e. Achromobacter liquidum
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.36
-
 [Flavobacterium] lutescens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.36 18.67
-
 pH 8.0, 37°C [Flavobacterium] lutescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.36 L-lysine + 2-oxoglutarate
-
 [Flavobacterium] lutescens 2-aminoadipate 6-semialdehyde + L-glutamate
-
 ?
2.6.1.36 L-lysine + 2-oxoglutarate 2-oxoglutarate is the only active amino acceptor [Flavobacterium] lutescens 2-aminoadipate 6-semialdehyde + L-glutamate
-
 ?
2.6.1.36 L-ornithine + 2-oxoglutarate
-
 [Flavobacterium] lutescens ? + L-glutamate
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.6.1.36 additional information
-
 pyridoxal 5'-phosphate, 0.1 mM, or 2-mercaptoethanol, 0.02%, protects against heat inactivation [Flavobacterium] lutescens
2.6.1.36 55
-
 pH 6.0-7.5, 5 min, stable, partially purified enzyme [Flavobacterium] lutescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.36 7.5
-
 substrates L-ornithine + 2-oxoglutarate [Flavobacterium] lutescens
2.6.1.36 8.3 8.5 substrates lysine + 2-oxoglutarate [Flavobacterium] lutescens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.6.1.36 6 9.5 about 40% of maximum activity at pH 6 and pH 9.5, substrates ornithine + 2-oxoglutarate [Flavobacterium] lutescens
2.6.1.36 7 10 pH 7: about 50% of maximum activity, pH 10: about 30% of maximum activity [Flavobacterium] lutescens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.6.1.36 6 7.5 55°C, 5 min, stable, partially purified enzyme [Flavobacterium] lutescens

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.36 pyridoxal 5'-phosphate a pyridoxal phosphate protein [Flavobacterium] lutescens
2.6.1.36 pyridoxal 5'-phosphate 2 mol of pyridoxal 5'-phosphate per mol of holoenzyme [Flavobacterium] lutescens
2.6.1.36 pyridoxal 5'-phosphate Km: 0.000357 mM [Flavobacterium] lutescens
2.6.1.36 pyridoxamine 5'-phosphate can replace pyridoxal 5'-phosphate, Km: 0.00715 mM [Flavobacterium] lutescens