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Literature summary extracted from

  • Hamada, S.; Nozaki, K.; Ito, H.; Yoshimoto, Y.; Yoshida, H.; Hiraga, S.; Onodera, S.; Honma, M.; Takeda, Y.; Matsui, H.
    Two starch-branching-enzyme isoforms occur in different fractions of developing seeds of kidney bean (2001), Biochem. J., 359, 23-34.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.18 citrate 0.3 M, more than 3fold stimulation of activity of isoenzyme SBE2, no effect on activity of isoenzyme SBE1 Phaseolus vulgaris

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.18 isoenzymes PySBE1 and PySBE2, expression in Escherichia coli Phaseolus vulgaris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.18 additional information
-
 additional information Km-value for amylose, isoenzyme SBE2: 1.27 mg/ml. Km-value for amylose, isoenzyme SBE1: 0.46 mg/ml Phaseolus vulgaris

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.18 soluble SBE2 is present in the soluble fraction, SBE1 is associated with the starch granule fraction Phaseolus vulgaris
-
-
2.4.1.18 starch grain SBE2 is present in the soluble fraction, SBE1 is associated with the starch granule fraction Phaseolus vulgaris 43036
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.18 81932
-
 1 * 81932, isoenzyme SBE2, calculation from nucleotide sequence Phaseolus vulgaris
2.4.1.18 82000
-
 1 * 82000, isoenzyme SBE2, SDS-PAGE Phaseolus vulgaris
2.4.1.18 88590
-
 1 * 88590, isoenzyme SBE1, calculation from nucleotide sequence Phaseolus vulgaris
2.4.1.18 100000
-
 1 * 100000, isoenzyme SBE1, SDS-PAGE Phaseolus vulgaris

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.18 Phaseolus vulgaris
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Subunits

EC Number Subunits Comment Organism
2.4.1.18 monomer 1 * 100000, isoenzyme SBE1, SDS-PAGE Phaseolus vulgaris
2.4.1.18 monomer 1 * 88590, isoenzyme SBE1, calculation from nucleotide sequence Phaseolus vulgaris
2.4.1.18 monomer 1 * 82000, isoenzyme SBE2, SDS-PAGE Phaseolus vulgaris
2.4.1.18 monomer 1 * 81932, isoenzyme SBE2, calculation from nucleotide sequence Phaseolus vulgaris

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.18 40
-
 pH 8.0, 15 min, stable up to, enzyme form KBE1 Phaseolus vulgaris
2.4.1.18 50
-
 pH 8.0, 15 min, stable up to, enzyme form KBE2 Phaseolus vulgaris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.18 7
-
-
 Phaseolus vulgaris

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.18 7 9 4°C, 16 h, less than 10% loss of activity, isoenzyme SBE2 Phaseolus vulgaris
2.4.1.18 7 8.5 4°C, 16 h, less than 10% loss of activity, isoenzyme SBE1 Phaseolus vulgaris