Any feedback?
Literature summary extracted from
- Properties of branching enzyme from hyperthermophilic bacterium, Aquifex aeolicus, and its potential for production of highly-branched cyclic dextrin (2003), J. Appl. Glycosci., 50, 15-20.
No PubMed abstract available
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 2.4.1.18 | expression in Escherichia coli | Aquifex aeolicus |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 2.4.1.18 | additional information | more than 95% of the recombinant enzyme is present within the cells as insoluble but catalytically active aggregate. Heat treatment of the aggregate suspension at 70°C results in about 30% solubilization of the enzyme activity | Aquifex aeolicus | - |
- |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.4.1.18 | Aquifex aeolicus | - |
- |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.4.1.18 | amylopectin | the enzyme cyclizes the B-chain which connects the cluster structures of amylopectin. The product, highly branched cyclic dextrin, has a ring structure with DPw 50 and non-cyclic chains with an average unit chain length of 16 connected to the ring | Aquifex aeolicus | amylopectin with additional alpha-1,6-glucosidic linkages | - |
? |  |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.18 | 75 | - |
soluble and insoluble enzyme form | Aquifex aeolicus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.18 | 70 | - |
pH 7.0, 30 min, 10% loss of the soluble enzyme form, 50% loss of the insoluble enzyme form | Aquifex aeolicus |
| 2.4.1.18 | 85 | - |
soluble and insoluble enzyme form, stable up to | Aquifex aeolicus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.18 | 7.5 | 8 | soluble and insoluble enzyme form | Aquifex aeolicus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
