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Literature summary extracted from

  • Boyer, C.; Preiss, J.
    Biosynthesis of bacterial glycogen. Purification and properties of the Escherichia coli b alpha-1,4-glucan: alpha-1,4-glucan 6-glycosyltansferase (1977), Biochemistry, 16, 3693-3699.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.18 84000
-
 1 * 84000, SDS-PAGE Escherichia coli
2.4.1.18 87000
-
 branching enzyme II, sucrose density gradient centrifugation Escherichia coli
2.4.1.18 95000
-
 branching enzyme I, sucrose density gradient centrifugation Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.18 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.18
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.18 299
-
 branching enzyme II Escherichia coli
2.4.1.18 460
-
 branching enzyme I Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.4.1.18 -85°C or 4°C, stable for several months Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.18 amylopectin
-
 Escherichia coli amylopectin with additional alpha-1,6-glucosidic linkages
-
 ?
2.4.1.18 amylose
-
 Escherichia coli amylose containing alpha-1,6-glucosidic linkages characterization of products, smallest chains transferred contain 5-7 glucose units ?
2.4.1.18 Glycogen from E. coli and rabbit liver, little activity Escherichia coli ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.18 monomer 1 * 84000, SDS-PAGE Escherichia coli