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Literature summary extracted from
- Biosynthesis of glycogen in Neurospora crassa. Purification and properties of the branching enzyme (1983), J. Biochem., 94, 451-458.
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.18 | 80000 | - |
native PAGE | Neurospora crassa |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.4.1.18 | Neurospora crassa | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 2.4.1.18 | glycoprotein | enzyme contains 0.03 mg of carbohydrate in 1 mg of enzyme protein | Neurospora crassa |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 2.4.1.18 | - |
Neurospora crassa |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 2.4.1.18 | mycelium | - |
Neurospora crassa | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.18 | additional information | - |
- |
Neurospora crassa |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 2.4.1.18 | -20°C, 50 mM glycylglycine buffer, pH 8.0, 5 mM mercaptoethanol, 1 mM EDTA, 0.02% NaN3, 25% glycerol | Neurospora crassa |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.4.1.18 | amylopectin | - |
Neurospora crassa | amylopectin with additional alpha-1,6-glucosidic linkages | - |
? |  |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 2.4.1.18 | monomer | 1 * 80000, SDS-PAGE | Neurospora crassa |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.18 | 27 | - |
- |
Neurospora crassa |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.18 | 35 | - |
pH 8.0, 30 min, stable below | Neurospora crassa |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.18 | 8 | - |
- |
Neurospora crassa |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.18 | 7.3 | 8.1 | 4°C, 16 h, stable | Neurospora crassa |
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