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Literature summary extracted from

  • Johnson, C.L.; Pechonick, E.; Park, S.D.; Havemann, G.D.; Leal, N.A.; Bobik, T.A.
    Functional genomic, biochemical, and genetic characterization of the Salmonella pduO gene, an ATP:cob(I)alamin adenosyltransferase gene (2001), J. Bacteriol., 183, 1577-1584.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.17 structural gene cobA Salmonella enterica
2.5.1.17 structural gene pduO Salmonella enterica subsp. enterica serovar Typhimurium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.17 K+
-
 Salmonella enterica
2.5.1.17 K+
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.17 Mg2+
-
 Salmonella enterica
2.5.1.17 Mg2+
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.17 36600
-
 calculated from amino acid sequence Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.17 37000
-
 SDS-PAGE of recombinant enzyme expressed in E. coli Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.17 ATP + cob(I)alamin Salmonella enterica subsp. enterica serovar Typhimurium the main role of this enzyme is apparently the conversion of inactive cobalamins to adenosyl cobalamin for 1,2 propanediol degradation triphosphate + coenzyme B12
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.17 Salmonella enterica
-
 serovar typhimurium
-
2.5.1.17 Salmonella enterica subsp. enterica serovar Typhimurium P31570 serovar typhimurium, structural gene pduO, this DNA sequence encodes for several enzymes
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.17 0.312
-
 pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.17 0.312
-
 activity of inclusion bodies formed with enzyme expressed in E.coli Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.17 ATP + cob(I)alamin
-
 Salmonella enterica triphosphate + coenzyme B12 adenosylcobalamin, B12-coenzyme or deoxyadenosyl-B12 ?
2.5.1.17 ATP + cob(I)alamin
-
 Salmonella enterica subsp. enterica serovar Typhimurium triphosphate + coenzyme B12 adenosylcobalamin, B12-coenzyme or deoxyadenosyl-B12 ?
2.5.1.17 ATP + cob(I)alamin the main role of this enzyme is apparently the conversion of inactive cobalamins to adenosyl cobalamin for 1,2 propanediol degradation Salmonella enterica subsp. enterica serovar Typhimurium triphosphate + coenzyme B12
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.17 37
-
 assay at Salmonella enterica
2.5.1.17 37
-
 assay at Salmonella enterica subsp. enterica serovar Typhimurium