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Literature summary extracted from

  • Costello, C.A.; Kelleher, N.L.; Abe, M.; McLafferty, F.W.; Begley, T.P.
    Mechanistic studies on thiaminase I. Overexpression and identification of the active site nucleophile (1996), J. Biol. Chem., 271, 3445-3452.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.2 cloned in Escherichia coli with Gly6 changed to Ala6 with no apparent effect Paenibacillus thiaminolyticus

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.2 C113S inactive mutant at the active nucleophilic site Paenibacillus thiaminolyticus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.2 4-Amino-6-chloro-2-methylpyrimidine inhibitory Paenibacillus thiaminolyticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.2 0.0037
-
 thiamine
-
 Paenibacillus thiaminolyticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.2 42000
-
 SDS-PAGE Paenibacillus thiaminolyticus

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.2 Paenibacillus thiaminolyticus P45741 cloned in Escherichia coli
-

Oxidation Stability

EC Number Oxidation Stability Organism
2.5.1.2 addition of dithiothreitol, 2 mM, reduces the loss of activity Paenibacillus thiaminolyticus

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.2 by ammonium precipitation, DEAE column, Cibacron blue dye affinity column Paenibacillus thiaminolyticus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.2 cell culture
-
 Paenibacillus thiaminolyticus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.2 thiamine + veratrylamine the reaction can occur without base substrate Paenibacillus thiaminolyticus bis-(4-amino-2-methyl)pyrimidinyl-3,4-dimethoxybenzylamine + 4-methyl-5-(2-hydroxyethyl)-thiazole
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.2 monomer 1 * 42000, SDS-polyacrylamide gel electrophoresis Paenibacillus thiaminolyticus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.2 25
-
 in 50 mM sodium phosphate at pH 7 Paenibacillus thiaminolyticus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.5.1.2 7
-
 50 mM sodium phosphate buffer, 2 mM dithiothreitol, 2 mM EDTA Paenibacillus thiaminolyticus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.2 7.7
-
 4-Amino-6-chloro-2-methylpyrimidine
-
 Paenibacillus thiaminolyticus