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Literature summary extracted from

  • Hutter, J.A.; Slama, J.T.
    Inhibition of thiaminase I from Bacillus thiaminolyticus. Evidence supporting a covalent 1,6-dihydropyrimidinyl-enzyme intermediate (1987), Biochemistry, 26, 1969-1973.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.2 4-Amino-5-(anilinomethyl)-6-chloro-2-methylpyrimidine complete irreversible inhibition 2 times faster than 4-amino-6-chloro-2-methylpyrimidine Paenibacillus thiaminolyticus
2.5.1.2 4-Amino-6-chloro-2-methylpyrimidine complete irreversible inhibition, activity protected by thiamine and quinolone Paenibacillus thiaminolyticus
2.5.1.2 additional information 4-amino-2-methyl pyrimidine does not inactivate nor competitively inhibits the enzyme Paenibacillus thiaminolyticus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.2 extracellular
-
 Paenibacillus thiaminolyticus
-
-

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.2 Paenibacillus thiaminolyticus
-
 BMM strain
-
2.5.1.2 Paenibacillus thiaminolyticus BMM
-
 BMM strain
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.2 cell culture
-
 Paenibacillus thiaminolyticus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.2 thiamine + aniline
-
 Paenibacillus thiaminolyticus 5-(anilinomethyl)-2-methylpyrimidin-4-amine + 4-methyl-5-(2-hydroxyethyl)-thiazole
-
 ?
2.5.1.2 thiamine + aniline
-
 Paenibacillus thiaminolyticus BMM 5-(anilinomethyl)-2-methylpyrimidin-4-amine + 4-methyl-5-(2-hydroxyethyl)-thiazole
-
 ?
2.5.1.2 thiamine + quinoline
-
 Paenibacillus thiaminolyticus 4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]quinolinium
-
 ?
2.5.1.2 thiamine + quinoline
-
 Paenibacillus thiaminolyticus BMM 4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]quinolinium
-
 ?

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.2 0.096
-
 4-Amino-5-(anilinomethyl)-6-chloro-2-methylpyrimidine
-
 Paenibacillus thiaminolyticus