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Literature summary extracted from

  • Suzuki, K.; Ooba, J.I.
    Reversible inactivation of extracellular thiaminase I in Bacillus thiaminolyticus. I. Inactivation by the primary substrate and reactivation by the secondary substrate (1973), Biochim. Biophys. Acta, 293, 111-117.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.5.1.2 2-mercaptoethanol similar effects as pyridine at 10 mM probably due to its action as secondary substrate Paenibacillus thiaminolyticus
2.5.1.2 4,5-dimethylthiazole reactivation at 10 mM Paenibacillus thiaminolyticus
2.5.1.2 5-(2-chloroethyl)-4-methylthiazole reactivation at 10 mM Paenibacillus thiaminolyticus
2.5.1.2 5-(2-hydroxyethyl)-4-methylthiazole reactivation at 10 mM Paenibacillus thiaminolyticus
2.5.1.2 aniline reactivation of enzyme at 2 mM Paenibacillus thiaminolyticus
2.5.1.2 dithiothreitol similar effects as pyridine at 10 mM probably due to its action as secondary substrate Paenibacillus thiaminolyticus
2.5.1.2 Pyridine at 25 mM restores activity inhibited by heteropyrimidine at 0.006 mM Paenibacillus thiaminolyticus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.2 aniline inactivation at 0.006 mM; reactivation at 2 mM Paenibacillus thiaminolyticus
2.5.1.2 Dimethialium inactivation at 0.006 mM Paenibacillus thiaminolyticus
2.5.1.2 Heteropyrithiamine inactivation at 0.006 mM that is restored by pyridine at 25 mM and inhibition of restoration of activity by pyridine at 1 mM Paenibacillus thiaminolyticus
2.5.1.2 additional information primary substrates inactivate the enzyme at 0.006 mM, the lost activity can be restored by incubation at 37°C for 60 min after dialysis or by incubation with secondary substrates or acceptor bases, each substrate at high concentrations abolishes the inactivation of the other substrate Paenibacillus thiaminolyticus
2.5.1.2 p-chloromercuribenzoate 50% of inhibition at 0.2 mM and inhibits reactivation by pyridine Paenibacillus thiaminolyticus
2.5.1.2 thiamine inactivation at 0.006 mM Paenibacillus thiaminolyticus
2.5.1.2 thiamine diphosphate inactivation at 0.006 mM Paenibacillus thiaminolyticus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.2 extracellular
-
 Paenibacillus thiaminolyticus
-
-

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.2 Paenibacillus thiaminolyticus
-
-
-
2.5.1.2 Paenibacillus thiaminolyticus YUSM I00I
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.2 cell culture
-
 Paenibacillus thiaminolyticus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.2 additional information primary substrates inactivate the enzyme at 0.006 mM and secondary substrates or acceptor bases reactivate the enzyme at 10 mM, each substrate at high concentrations eliminates the inactivation of the other substrate Paenibacillus thiaminolyticus ?
-
 ?
2.5.1.2 additional information primary substrates inactivate the enzyme at 0.006 mM and secondary substrates or acceptor bases reactivate the enzyme at 10 mM, each substrate at high concentrations eliminates the inactivation of the other substrate Paenibacillus thiaminolyticus YUSM I00I ?
-
 ?
2.5.1.2 thiamine + aniline
-
 Paenibacillus thiaminolyticus 5-(anilinomethyl)-2-methylpyrimidin-4-amine + 4-methyl-5-(2-hydroxyethyl)-thiazole
-
 ?
2.5.1.2 thiamine + aniline
-
 Paenibacillus thiaminolyticus YUSM I00I 5-(anilinomethyl)-2-methylpyrimidin-4-amine + 4-methyl-5-(2-hydroxyethyl)-thiazole
-
 ?
2.5.1.2 thiamine + pyridine
-
 Paenibacillus thiaminolyticus heteropyrithiamine + 4-methyl-5-(2-hydroxyethyl)-thiazole
-
 ?
2.5.1.2 thiamine + pyridine
-
 Paenibacillus thiaminolyticus YUSM I00I heteropyrithiamine + 4-methyl-5-(2-hydroxyethyl)-thiazole
-
 ?

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.5.1.2 37
-
 assay at Paenibacillus thiaminolyticus
2.5.1.2 37
-
 after dialysis, 60 min of incubation restore 90% of activity inhibited by thiamine Paenibacillus thiaminolyticus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.5.1.2 6.5
-
 0.15 M citrate-phosphate buffer Paenibacillus thiaminolyticus