BRENDA - Enzyme Database show

The single deoxynucleoside kinase in Drosophila melanogaster, Dm-dNK, is multifunctional and differs from the mammalian deoxynucleoside kinases

Munch-Petersen, B.; Piskur, J.; Sondergaard, L.; Adv. Exp. Med. Biol. 431, 465-469 (1998)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.145
2'-deoxycytidine
competitive with thymidine
Drosophila melanogaster
2.7.1.145
2'-deoxyguanosine
-
Drosophila melanogaster
2.7.1.145
thymidine
competitive with 2'-deoxycytidine
Drosophila melanogaster
2.7.1.145
TTP
feed-back inhibition
Drosophila melanogaster
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.145
0.001
-
2'-deoxycytidine
pH 8.0, 22°C
Drosophila melanogaster
2.7.1.145
0.001
-
thymidine
pH 8.0, 22°C
Drosophila melanogaster
2.7.1.145
0.0014
-
ATP
pH 8.0, 22°C, reaction with 2'-deoxycytidine
Drosophila melanogaster
2.7.1.145
0.0022
-
ATP
pH 8.0, 22°C, reaction with thymidine
Drosophila melanogaster
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.145
29000
-
1 * 29000, SDS-PAGE
Drosophila melanogaster
2.7.1.145
30000
-
gel filtration
Drosophila melanogaster
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.145
Drosophila melanogaster
-
S-2
-
2.7.1.145
Drosophila melanogaster S-2
-
S-2
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.1.145
-
Drosophila melanogaster
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.145
embryo
-
Drosophila melanogaster
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.145
30
-
-
Drosophila melanogaster
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.145
1-beta-D-arabinofuranosylcytosine + ATP
50% of the activity with thymidine and 2'-deoxycytidine
636839
Drosophila melanogaster
1-beta-D-arabinofuranosylcytosine 5'-phosphate + ADP
-
-
-
?
2.7.1.145
1-beta-D-arabinofuranosylthymine + ATP
15% of the activity with thymidine and 2'-deoxycytidine
636839
Drosophila melanogaster
1-beta-D-arabinofuranosylthymine 5'-phosphate + ADP
-
-
-
?
2.7.1.145
2'-deoxyadenosine + ATP
15% of the activity with thymidine and 2'-deoxycytidine
636839
Drosophila melanogaster
2'-deoxyadenosine 5'-phosphate + ADP
-
-
-
?
2.7.1.145
2'-deoxycytidine + ATP
-
636839
Drosophila melanogaster
2'-deoxycytidine 5'-phosphate + ADP
-
-
-
?
2.7.1.145
2'-deoxycytidine + ATP
-
636839
Drosophila melanogaster S-2
2'-deoxycytidine 5'-phosphate + ADP
-
-
-
?
2.7.1.145
2'-deoxyguanosine + ATP
4% of the activity with thymidine and 2'-deoxycytidine
636839
Drosophila melanogaster
2'-deoxyguanosine 5'-phosphate + ADP
-
-
-
?
2.7.1.145
3'-azidothymidine + ATP
0.3% of the activity with thymidine and 2'-deoxycytidine
636839
Drosophila melanogaster
3'-azidothymidine 5'-phosphate + ADP
-
-
-
?
2.7.1.145
additional information
no activity with acyclovir
636839
Drosophila melanogaster
?
-
-
-
-
2.7.1.145
additional information
no activity with TMP
636839
Drosophila melanogaster
?
-
-
-
-
2.7.1.145
additional information
CTP has the same phosphotransfer capability as ATP
636839
Drosophila melanogaster
?
-
-
-
-
2.7.1.145
additional information
no activity with acyclovir
636839
Drosophila melanogaster S-2
?
-
-
-
-
2.7.1.145
additional information
no activity with TMP
636839
Drosophila melanogaster S-2
?
-
-
-
-
2.7.1.145
additional information
CTP has the same phosphotransfer capability as ATP
636839
Drosophila melanogaster S-2
?
-
-
-
-
2.7.1.145
thymidine + ATP
-
636839
Drosophila melanogaster
thymidine 5'-phosphate + ADP
-
-
-
?
2.7.1.145
thymidine + ATP
-
636839
Drosophila melanogaster S-2
thymidine 5'-phosphate + ADP
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
2.7.1.145
monomer
1 * 29000, SDS-PAGE
Drosophila melanogaster
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.7.1.145
additional information
-
additional information
-
Drosophila melanogaster
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.1.145
0.001
-
2'-deoxycytidine
pH 8.0, 22°C, competitive with thymidine
Drosophila melanogaster
2.7.1.145
0.001
-
thymidine
pH 8.0, 22°C, competitive with 2'-deoxycytidine
Drosophila melanogaster
2.7.1.145
0.005
-
TTP
pH 8.0, 22°C
Drosophila melanogaster
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.145
2'-deoxycytidine
competitive with thymidine
Drosophila melanogaster
2.7.1.145
2'-deoxyguanosine
-
Drosophila melanogaster
2.7.1.145
thymidine
competitive with 2'-deoxycytidine
Drosophila melanogaster
2.7.1.145
TTP
feed-back inhibition
Drosophila melanogaster
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.1.145
0.001
-
2'-deoxycytidine
pH 8.0, 22°C, competitive with thymidine
Drosophila melanogaster
2.7.1.145
0.001
-
thymidine
pH 8.0, 22°C, competitive with 2'-deoxycytidine
Drosophila melanogaster
2.7.1.145
0.005
-
TTP
pH 8.0, 22°C
Drosophila melanogaster
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.145
0.001
-
2'-deoxycytidine
pH 8.0, 22°C
Drosophila melanogaster
2.7.1.145
0.001
-
thymidine
pH 8.0, 22°C
Drosophila melanogaster
2.7.1.145
0.0014
-
ATP
pH 8.0, 22°C, reaction with 2'-deoxycytidine
Drosophila melanogaster
2.7.1.145
0.0022
-
ATP
pH 8.0, 22°C, reaction with thymidine
Drosophila melanogaster
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.145
29000
-
1 * 29000, SDS-PAGE
Drosophila melanogaster
2.7.1.145
30000
-
gel filtration
Drosophila melanogaster
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.145
-
Drosophila melanogaster
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.145
embryo
-
Drosophila melanogaster
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.145
30
-
-
Drosophila melanogaster
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.145
1-beta-D-arabinofuranosylcytosine + ATP
50% of the activity with thymidine and 2'-deoxycytidine
636839
Drosophila melanogaster
1-beta-D-arabinofuranosylcytosine 5'-phosphate + ADP
-
-
-
?
2.7.1.145
1-beta-D-arabinofuranosylthymine + ATP
15% of the activity with thymidine and 2'-deoxycytidine
636839
Drosophila melanogaster
1-beta-D-arabinofuranosylthymine 5'-phosphate + ADP
-
-
-
?
2.7.1.145
2'-deoxyadenosine + ATP
15% of the activity with thymidine and 2'-deoxycytidine
636839
Drosophila melanogaster
2'-deoxyadenosine 5'-phosphate + ADP
-
-
-
?
2.7.1.145
2'-deoxycytidine + ATP
-
636839
Drosophila melanogaster
2'-deoxycytidine 5'-phosphate + ADP
-
-
-
?
2.7.1.145
2'-deoxycytidine + ATP
-
636839
Drosophila melanogaster S-2
2'-deoxycytidine 5'-phosphate + ADP
-
-
-
?
2.7.1.145
2'-deoxyguanosine + ATP
4% of the activity with thymidine and 2'-deoxycytidine
636839
Drosophila melanogaster
2'-deoxyguanosine 5'-phosphate + ADP
-
-
-
?
2.7.1.145
3'-azidothymidine + ATP
0.3% of the activity with thymidine and 2'-deoxycytidine
636839
Drosophila melanogaster
3'-azidothymidine 5'-phosphate + ADP
-
-
-
?
2.7.1.145
additional information
no activity with acyclovir
636839
Drosophila melanogaster
?
-
-
-
-
2.7.1.145
additional information
no activity with TMP
636839
Drosophila melanogaster
?
-
-
-
-
2.7.1.145
additional information
CTP has the same phosphotransfer capability as ATP
636839
Drosophila melanogaster
?
-
-
-
-
2.7.1.145
additional information
no activity with acyclovir
636839
Drosophila melanogaster S-2
?
-
-
-
-
2.7.1.145
additional information
no activity with TMP
636839
Drosophila melanogaster S-2
?
-
-
-
-
2.7.1.145
additional information
CTP has the same phosphotransfer capability as ATP
636839
Drosophila melanogaster S-2
?
-
-
-
-
2.7.1.145
thymidine + ATP
-
636839
Drosophila melanogaster
thymidine 5'-phosphate + ADP
-
-
-
?
2.7.1.145
thymidine + ATP
-
636839
Drosophila melanogaster S-2
thymidine 5'-phosphate + ADP
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.7.1.145
monomer
1 * 29000, SDS-PAGE
Drosophila melanogaster
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.7.1.145
additional information
-
additional information
-
Drosophila melanogaster