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Literature summary extracted from

  • de Silva, J.; Jarman, C.D.; Arrowsmith, D.A.; Stronach, M.S.; Chengappa, S.; Sidebottom, C.; Reid, J.S.G.
    Molecular characterization of a xyloglucan-specific endo-(1-->4)-beta-D-glucanase (xyloglucan endo-transglycosylase) from nasturtium seeds (1993), Plant J., 3, 701-711.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.207 single copy gene, cDNA encoding XET is cloned, sequenced and encodes a 33.5 kDa precursor polypeptide, which is subsequently processed to a 31 kDa mature protein, NXG1 and 2 may be different alleles of the XET gene Tropaeolum majus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.207 cell wall localized exclusively in Tropaeolum majus 5618
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.207 31000
-
 x * 31000, SDS-PAGE and calculated from the amino acid sequence, the cDNA encodes a 33.5 kDa precursor polypeptide, which is subsequently processed to a 31 kDa mature protein Tropaeolum majus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.207 donor xyloglucan + xyloglucan acceptor Tropaeolum majus enzyme is involved in the post-germinative mobilization of xyloglucan storage reserves, involved in cell wall loosening, activity is primarily regulated at the level of gene expression ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.207 Tropaeolum majus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.1.207 no glycoprotein
-
 Tropaeolum majus
2.4.1.207 proteolytic modification hydrophobic core of 24 amino acids at the N-terminus, characteristic of a signal peptide, enzyme must be transported through the cell membrane in order to reach the cell wall, DNA encodes a 33.5 kDa precursor polypeptide, which is subsequently processed to a 31 kDa mature protein Tropaeolum majus

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.207
-
 Tropaeolum majus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.207 cotyledon source for mRNA isolation Tropaeolum majus
-
2.4.1.207 seed
-
 Tropaeolum majus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.207 donor xyloglucan + acceptor xyloglucan specific for xyloglucan Tropaeolum majus ?
-
 ?
2.4.1.207 donor xyloglucan + xyloglucan acceptor enzyme is involved in the post-germinative mobilization of xyloglucan storage reserves, involved in cell wall loosening, activity is primarily regulated at the level of gene expression Tropaeolum majus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.207 ? x * 31000, SDS-PAGE and calculated from the amino acid sequence, the cDNA encodes a 33.5 kDa precursor polypeptide, which is subsequently processed to a 31 kDa mature protein Tropaeolum majus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.207 additional information
-
 pI: 5.0-5.14 Tropaeolum majus