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Literature summary extracted from

  • Myllylä, R.; Anttinen, H.; Risteli, L.; Kivirikko, K.I.
    Isolation of collagen glucosyltransferase as a homogeneous protein from chick embryos (1977), Biochim. Biophys. Acta, 480, 113-121.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.4.1.66 concentrated enzyme, above 0.2 mg/ml, loses most of the activity Gallus gallus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.66 Mn2+
-
 Gallus gallus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.66 Gallus gallus
-
 white Leghorn chicken
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.1.66 glycoprotein
-
 Gallus gallus

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.66 14600fold Gallus gallus
2.4.1.66 affinity chromatography Gallus gallus
2.4.1.66 solubilized with 0.5 M NaCl, 2 M urea increases solubilization rate Gallus gallus
2.4.1.66 solubilized with Nonidet P-40 Gallus gallus
2.4.1.66 solubilized with Triton X-100 Gallus gallus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.66 embryo 14-15 days old Gallus gallus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.66 0.43
-
-
 Gallus gallus

Storage Stability

EC Number Storage Stability Organism
2.4.1.66 -20°C, enzyme concentration 0.1 mg/ml, several months, 33% loss of activity Gallus gallus
2.4.1.66 4°C, enzyme concentration 0.1 mg/ml, 1 week, 33% loss of activity Gallus gallus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.66 additional information not: UDP-N-acetylglucosamine and UDP-N-acetylgalactosamine Gallus gallus ?
-
 ?
2.4.1.66 additional information not: ADPglucose Gallus gallus ?
-
 ?
2.4.1.66 UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen heat-denatured citrate-soluble rat skin collagen Gallus gallus UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen
-
 ?
2.4.1.66 UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen calf skin gelatin Gallus gallus UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.66 monomer 1 * 72000-78000, value depends on the gel composition, SDS-PAGE Gallus gallus