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Literature summary extracted from

  • Jensen, J.W.; Schutzbach, J.S.
    The biosynthesis of oligosaccharide-lipids. Partial purification and characterization of mannosyltransferase II (1981), J. Biol. Chem., 256, 12899-12904.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.132 dolichol stimulates enzyme in the presence of inhibitory concentrations of phosphatidylcholine Oryctolagus cuniculus
2.4.1.132 dolichyl phosphate stimulates enzyme in the presence of inhibitory concentrations of phosphatidylcholine Oryctolagus cuniculus
2.4.1.132 Nonidet P-40 activation, up to 0.0225% v/v Oryctolagus cuniculus

General Stability

EC Number General Stability Organism
2.4.1.132 glycerol, 0.5% v/v, stabilizes during storage and purification Oryctolagus cuniculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.132 ADP weak Oryctolagus cuniculus
2.4.1.132 ATP weak Oryctolagus cuniculus
2.4.1.132 EDTA
-
 Oryctolagus cuniculus
2.4.1.132 GDP 50% inhibition at 0.0006 mM Oryctolagus cuniculus
2.4.1.132 GDPglucose
-
 Oryctolagus cuniculus
2.4.1.132 glycerol 1-2% v/v Oryctolagus cuniculus
2.4.1.132 GMP 50% inhibition at 0.094 mM Oryctolagus cuniculus
2.4.1.132 GTP 50% inhibition at 0.006 mM Oryctolagus cuniculus
2.4.1.132 additional information no inhibition by amphomycin Oryctolagus cuniculus
2.4.1.132 NiCl2
-
 Oryctolagus cuniculus
2.4.1.132 UDP weak Oryctolagus cuniculus
2.4.1.132 UDP-N-acetylglucosamine weak Oryctolagus cuniculus
2.4.1.132 UDPglucose weak Oryctolagus cuniculus
2.4.1.132 UTP weak Oryctolagus cuniculus
2.4.1.132 ZnCl2
-
 Oryctolagus cuniculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.132 0.00021
-
 tetrasaccharide-diphosphoryl-lipid detergent-stimulated Oryctolagus cuniculus
2.4.1.132 0.00065
-
 GDPmannose detergent-stimulated Oryctolagus cuniculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.132 microsome
-
 Oryctolagus cuniculus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.132 Ca2+ activation, about 60% as effective as Mg2+ Oryctolagus cuniculus
2.4.1.132 Co2+ activation, about 4% as effective as Mg2+ Oryctolagus cuniculus
2.4.1.132 Mg2+ requirement Oryctolagus cuniculus
2.4.1.132 Mg2+ 10 mM Oryctolagus cuniculus
2.4.1.132 Mn2+ activation, about 40% as effective as Mg2+ Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.132 GDPmannose + tetrasaccharide-diphosphoryl-lipid Oryctolagus cuniculus involved in the biosynthesis of asparagine-linked saccharide chains of mammalian glycoproteins GDP + mannosyl-alpha-1,3-tetrasaccharide-diphosphoryl-lipid
-
 ?
2.4.1.132 GDPmannose + tetrasaccharide-diphosphoryl-lipid Oryctolagus cuniculus the 1,3-linked mannosyl residue in mammalian lipid-linked oligosaccharide of the structure Glc3Man9GlcNAc2 is produced by this enzyme GDP + mannosyl-alpha-1,3-tetrasaccharide-diphosphoryl-lipid
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.132 Oryctolagus cuniculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.132 partial Oryctolagus cuniculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.132 liver
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.132 additional information
-
-
 Oryctolagus cuniculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.132 GDPmannose + tetrasaccharide-diphosphoryl-lipid no acceptor is dolichol-phosphate Oryctolagus cuniculus GDP + mannosyl-alpha-1,3-tetrasaccharide-diphosphoryl-lipid
-
 ?
2.4.1.132 GDPmannose + tetrasaccharide-diphosphoryl-lipid involved in the biosynthesis of asparagine-linked saccharide chains of mammalian glycoproteins Oryctolagus cuniculus GDP + mannosyl-alpha-1,3-tetrasaccharide-diphosphoryl-lipid
-
 ?
2.4.1.132 GDPmannose + tetrasaccharide-diphosphoryl-lipid the 1,3-linked mannosyl residue in mammalian lipid-linked oligosaccharide of the structure Glc3Man9GlcNAc2 is produced by this enzyme Oryctolagus cuniculus GDP + mannosyl-alpha-1,3-tetrasaccharide-diphosphoryl-lipid
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.132 37
-
 assay at Oryctolagus cuniculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.132 6.8 7.3
-
 Oryctolagus cuniculus