BRENDA - Enzyme Database

The biosynthesis of oligosaccharide-lipids. Partial purification and characterization of mannosyltransferase II

Jensen, J.W.; Schutzbach, J.S.; J. Biol. Chem. 256, 12899-12904 (1981)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.4.1.132
dolichol
stimulates enzyme in the presence of inhibitory concentrations of phosphatidylcholine
Oryctolagus cuniculus
2.4.1.132
dolichyl phosphate
stimulates enzyme in the presence of inhibitory concentrations of phosphatidylcholine
Oryctolagus cuniculus
2.4.1.132
Nonidet P-40
activation, up to 0.0225% v/v
Oryctolagus cuniculus
General Stability
EC Number
General Stability
Organism
2.4.1.132
glycerol, 0.5% v/v, stabilizes during storage and purification
Oryctolagus cuniculus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.1.132
ADP
weak
Oryctolagus cuniculus
2.4.1.132
ATP
weak
Oryctolagus cuniculus
2.4.1.132
EDTA
-
Oryctolagus cuniculus
2.4.1.132
GDP
50% inhibition at 0.0006 mM
Oryctolagus cuniculus
2.4.1.132
GDPglucose
-
Oryctolagus cuniculus
2.4.1.132
glycerol
1-2% v/v
Oryctolagus cuniculus
2.4.1.132
GMP
50% inhibition at 0.094 mM
Oryctolagus cuniculus
2.4.1.132
GTP
50% inhibition at 0.006 mM
Oryctolagus cuniculus
2.4.1.132
additional information
no inhibition by amphomycin
Oryctolagus cuniculus
2.4.1.132
NiCl2
-
Oryctolagus cuniculus
2.4.1.132
UDP
weak
Oryctolagus cuniculus
2.4.1.132
UDP-N-acetylglucosamine
weak
Oryctolagus cuniculus
2.4.1.132
UDPglucose
weak
Oryctolagus cuniculus
2.4.1.132
UTP
weak
Oryctolagus cuniculus
2.4.1.132
ZnCl2
-
Oryctolagus cuniculus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.1.132
0.00021
-
tetrasaccharide-diphosphoryl-lipid
detergent-stimulated
Oryctolagus cuniculus
2.4.1.132
0.00065
-
GDPmannose
detergent-stimulated
Oryctolagus cuniculus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.1.132
microsome
-
Oryctolagus cuniculus
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.1.132
Ca2+
activation, about 60% as effective as Mg2+
Oryctolagus cuniculus
2.4.1.132
Co2+
activation, about 4% as effective as Mg2+
Oryctolagus cuniculus
2.4.1.132
Mg2+
10 mM; requirement
Oryctolagus cuniculus
2.4.1.132
Mn2+
activation, about 40% as effective as Mg2+
Oryctolagus cuniculus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.4.1.132
GDPmannose + tetrasaccharide-diphosphoryl-lipid
Oryctolagus cuniculus
involved in the biosynthesis of asparagine-linked saccharide chains of mammalian glycoproteins
GDP + mannosyl-alpha-1,3-tetrasaccharide-diphosphoryl-lipid
-
-
-
2.4.1.132
GDPmannose + tetrasaccharide-diphosphoryl-lipid
Oryctolagus cuniculus
the 1,3-linked mannosyl residue in mammalian lipid-linked oligosaccharide of the structure Glc3Man9GlcNAc2 is produced by this enzyme
GDP + mannosyl-alpha-1,3-tetrasaccharide-diphosphoryl-lipid
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.1.132
Oryctolagus cuniculus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.4.1.132
partial
Oryctolagus cuniculus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.1.132
liver
-
Oryctolagus cuniculus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.4.1.132
additional information
-
-
Oryctolagus cuniculus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.132
GDPmannose + tetrasaccharide-diphosphoryl-lipid
no acceptor is dolichol-phosphate
636752
Oryctolagus cuniculus
GDP + mannosyl-alpha-1,3-tetrasaccharide-diphosphoryl-lipid
-
636752
Oryctolagus cuniculus
?
2.4.1.132
GDPmannose + tetrasaccharide-diphosphoryl-lipid
involved in the biosynthesis of asparagine-linked saccharide chains of mammalian glycoproteins
636752
Oryctolagus cuniculus
GDP + mannosyl-alpha-1,3-tetrasaccharide-diphosphoryl-lipid
-
-
-
-
2.4.1.132
GDPmannose + tetrasaccharide-diphosphoryl-lipid
the 1,3-linked mannosyl residue in mammalian lipid-linked oligosaccharide of the structure Glc3Man9GlcNAc2 is produced by this enzyme
636752
Oryctolagus cuniculus
GDP + mannosyl-alpha-1,3-tetrasaccharide-diphosphoryl-lipid
-
-
-
-
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.4.1.132
37
-
assay at
Oryctolagus cuniculus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.1.132
6.8
7.3
-
Oryctolagus cuniculus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.4.1.132
dolichol
stimulates enzyme in the presence of inhibitory concentrations of phosphatidylcholine
Oryctolagus cuniculus
2.4.1.132
dolichyl phosphate
stimulates enzyme in the presence of inhibitory concentrations of phosphatidylcholine
Oryctolagus cuniculus
2.4.1.132
Nonidet P-40
activation, up to 0.0225% v/v
Oryctolagus cuniculus
General Stability (protein specific)
EC Number
General Stability
Organism
2.4.1.132
glycerol, 0.5% v/v, stabilizes during storage and purification
Oryctolagus cuniculus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.1.132
ADP
weak
Oryctolagus cuniculus
2.4.1.132
ATP
weak
Oryctolagus cuniculus
2.4.1.132
EDTA
-
Oryctolagus cuniculus
2.4.1.132
GDP
50% inhibition at 0.0006 mM
Oryctolagus cuniculus
2.4.1.132
GDPglucose
-
Oryctolagus cuniculus
2.4.1.132
glycerol
1-2% v/v
Oryctolagus cuniculus
2.4.1.132
GMP
50% inhibition at 0.094 mM
Oryctolagus cuniculus
2.4.1.132
GTP
50% inhibition at 0.006 mM
Oryctolagus cuniculus
2.4.1.132
additional information
no inhibition by amphomycin
Oryctolagus cuniculus
2.4.1.132
NiCl2
-
Oryctolagus cuniculus
2.4.1.132
UDP
weak
Oryctolagus cuniculus
2.4.1.132
UDP-N-acetylglucosamine
weak
Oryctolagus cuniculus
2.4.1.132
UDPglucose
weak
Oryctolagus cuniculus
2.4.1.132
UTP
weak
Oryctolagus cuniculus
2.4.1.132
ZnCl2
-
Oryctolagus cuniculus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.1.132
0.00021
-
tetrasaccharide-diphosphoryl-lipid
detergent-stimulated
Oryctolagus cuniculus
2.4.1.132
0.00065
-
GDPmannose
detergent-stimulated
Oryctolagus cuniculus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.1.132
microsome
-
Oryctolagus cuniculus
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.1.132
Ca2+
activation, about 60% as effective as Mg2+
Oryctolagus cuniculus
2.4.1.132
Co2+
activation, about 4% as effective as Mg2+
Oryctolagus cuniculus
2.4.1.132
Mg2+
10 mM; requirement
Oryctolagus cuniculus
2.4.1.132
Mn2+
activation, about 40% as effective as Mg2+
Oryctolagus cuniculus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.4.1.132
GDPmannose + tetrasaccharide-diphosphoryl-lipid
Oryctolagus cuniculus
involved in the biosynthesis of asparagine-linked saccharide chains of mammalian glycoproteins
GDP + mannosyl-alpha-1,3-tetrasaccharide-diphosphoryl-lipid
-
-
-
2.4.1.132
GDPmannose + tetrasaccharide-diphosphoryl-lipid
Oryctolagus cuniculus
the 1,3-linked mannosyl residue in mammalian lipid-linked oligosaccharide of the structure Glc3Man9GlcNAc2 is produced by this enzyme
GDP + mannosyl-alpha-1,3-tetrasaccharide-diphosphoryl-lipid
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.4.1.132
partial
Oryctolagus cuniculus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.1.132
liver
-
Oryctolagus cuniculus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.4.1.132
additional information
-
-
Oryctolagus cuniculus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.132
GDPmannose + tetrasaccharide-diphosphoryl-lipid
no acceptor is dolichol-phosphate
636752
Oryctolagus cuniculus
GDP + mannosyl-alpha-1,3-tetrasaccharide-diphosphoryl-lipid
-
636752
Oryctolagus cuniculus
?
2.4.1.132
GDPmannose + tetrasaccharide-diphosphoryl-lipid
involved in the biosynthesis of asparagine-linked saccharide chains of mammalian glycoproteins
636752
Oryctolagus cuniculus
GDP + mannosyl-alpha-1,3-tetrasaccharide-diphosphoryl-lipid
-
-
-
-
2.4.1.132
GDPmannose + tetrasaccharide-diphosphoryl-lipid
the 1,3-linked mannosyl residue in mammalian lipid-linked oligosaccharide of the structure Glc3Man9GlcNAc2 is produced by this enzyme
636752
Oryctolagus cuniculus
GDP + mannosyl-alpha-1,3-tetrasaccharide-diphosphoryl-lipid
-
-
-
-
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.4.1.132
37
-
assay at
Oryctolagus cuniculus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.1.132
6.8
7.3
-
Oryctolagus cuniculus