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Literature summary extracted from

  • Ro, H.S.; Hong, S.P.; Seo, H.J.; Yoshimura, T.; Esaki, N.; Soda, K.; Kim, H.S.; Sung, M.H.
    Site-directed mutagenesis of the amino acid residues in beta-strand III [Val30-Val36] of D-amino acid aminotransferase of Bacillus sp. YM-1 (1996), FEBS Lett., 398, 141-145.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.21
-
Bacillus sp. (in: Bacteria)

Protein Variants

EC Number Protein Variants Comment Organism
2.6.1.21 E32A 1% of wild-type activity Bacillus sp. (in: Bacteria)
2.6.1.21 E32D 65% of wild-type activity Bacillus sp. (in: Bacteria)
2.6.1.21 E32Q 10% of wild-type activity Bacillus sp. (in: Bacteria)
2.6.1.21 K33A 64% of wild-type activity Bacillus sp. (in: Bacteria)
2.6.1.21 V33A 110% of wild-type activity Bacillus sp. (in: Bacteria)
2.6.1.21 Y31A 0.4% of wild-type activity Bacillus sp. (in: Bacteria)

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.21 1.2
-
D-alanine pH 8.5, 50°C, E32D mtant enzyme Bacillus sp. (in: Bacteria)
2.6.1.21 1.4
-
2-oxoglutarate pH 8.5, 50°C, E32Q mutant enzyme Bacillus sp. (in: Bacteria)
2.6.1.21 1.8
-
2-oxoglutarate pH 8.5, 50°C, E32A mutant enzyme Bacillus sp. (in: Bacteria)
2.6.1.21 2.1
-
D-alanine pH 8.5, 50°C, wild-type and E32Q mutant enzyme Bacillus sp. (in: Bacteria)
2.6.1.21 2.1
-
2-oxoglutarate pH 8.5, 50°C, E32D mutant enzyme Bacillus sp. (in: Bacteria)
2.6.1.21 2.4
-
2-oxoglutarate pH 8.5, 50°C, Y33A mutant enzyme Bacillus sp. (in: Bacteria)
2.6.1.21 2.5
-
2-oxoglutarate pH 8.5, 50°C, wild-type enzyme Bacillus sp. (in: Bacteria)
2.6.1.21 10.1
-
2-oxoglutarate pH 8.5, 50°C, Y31A mutant enzyme Bacillus sp. (in: Bacteria)
2.6.1.21 15.5
-
2-oxoisovalerate pH 8.5, 50°C, V33A mutant enzyme Bacillus sp. (in: Bacteria)
2.6.1.21 28
-
2-oxoisovalerate pH 8.5, 50°C, wild-type enzyme Bacillus sp. (in: Bacteria)
2.6.1.21 32.2
-
2-oxoisovalerate pH 8.5, 50°C, K35A mutant enzyme Bacillus sp. (in: Bacteria)
2.6.1.21 35.6
-
2-oxoglutarate pH 8.5, 50°C, K35A mutant enzyme Bacillus sp. (in: Bacteria)

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.21 Bacillus sp. (in: Bacteria)
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.21 recombinant wild-type and mutant enzymes, Resource Q, phenyl-superose Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.21 D-alanine + 2-oxoisovalerate
-
Bacillus sp. (in: Bacteria) pyruvate + D-valine
-
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Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.6.1.21 0.5
-
D-alanine pH 8.5, 50°C, Y31A mutant enzyme Bacillus sp. (in: Bacteria)
2.6.1.21 1.3
-
D-alanine pH 8.5, 50°C, E32A mutant enzyme Bacillus sp. (in: Bacteria)
2.6.1.21 13.4
-
D-alanine pH 8.5, 50°C, E32Q mutant enzyme Bacillus sp. (in: Bacteria)
2.6.1.21 84.3
-
D-alanine pH 8.5, 50°C, K35A mutant enzyme Bacillus sp. (in: Bacteria)
2.6.1.21 85.6
-
D-alanine pH 8.5, 50°C, E32D mutant enzyme Bacillus sp. (in: Bacteria)
2.6.1.21 131
-
D-alanine pH 8.5, 50°C, wild-type enzyme Bacillus sp. (in: Bacteria)
2.6.1.21 145
-
D-alanine pH 8.5, 50°C, V33A mutant enzyme Bacillus sp. (in: Bacteria)