EC Number | Cloned (Comment) | Organism |
---|---|---|
2.6.1.21 | - |
Bacillus sp. (in: Bacteria) |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.6.1.21 | E32A | 1% of wild-type activity | Bacillus sp. (in: Bacteria) |
2.6.1.21 | E32D | 65% of wild-type activity | Bacillus sp. (in: Bacteria) |
2.6.1.21 | E32Q | 10% of wild-type activity | Bacillus sp. (in: Bacteria) |
2.6.1.21 | K33A | 64% of wild-type activity | Bacillus sp. (in: Bacteria) |
2.6.1.21 | V33A | 110% of wild-type activity | Bacillus sp. (in: Bacteria) |
2.6.1.21 | Y31A | 0.4% of wild-type activity | Bacillus sp. (in: Bacteria) |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.6.1.21 | 1.2 | - |
D-alanine | pH 8.5, 50°C, E32D mtant enzyme | Bacillus sp. (in: Bacteria) | |
2.6.1.21 | 1.4 | - |
2-oxoglutarate | pH 8.5, 50°C, E32Q mutant enzyme | Bacillus sp. (in: Bacteria) | |
2.6.1.21 | 1.8 | - |
2-oxoglutarate | pH 8.5, 50°C, E32A mutant enzyme | Bacillus sp. (in: Bacteria) | |
2.6.1.21 | 2.1 | - |
D-alanine | pH 8.5, 50°C, wild-type and E32Q mutant enzyme | Bacillus sp. (in: Bacteria) | |
2.6.1.21 | 2.1 | - |
2-oxoglutarate | pH 8.5, 50°C, E32D mutant enzyme | Bacillus sp. (in: Bacteria) | |
2.6.1.21 | 2.4 | - |
2-oxoglutarate | pH 8.5, 50°C, Y33A mutant enzyme | Bacillus sp. (in: Bacteria) | |
2.6.1.21 | 2.5 | - |
2-oxoglutarate | pH 8.5, 50°C, wild-type enzyme | Bacillus sp. (in: Bacteria) | |
2.6.1.21 | 10.1 | - |
2-oxoglutarate | pH 8.5, 50°C, Y31A mutant enzyme | Bacillus sp. (in: Bacteria) | |
2.6.1.21 | 15.5 | - |
2-oxoisovalerate | pH 8.5, 50°C, V33A mutant enzyme | Bacillus sp. (in: Bacteria) | |
2.6.1.21 | 28 | - |
2-oxoisovalerate | pH 8.5, 50°C, wild-type enzyme | Bacillus sp. (in: Bacteria) | |
2.6.1.21 | 32.2 | - |
2-oxoisovalerate | pH 8.5, 50°C, K35A mutant enzyme | Bacillus sp. (in: Bacteria) | |
2.6.1.21 | 35.6 | - |
2-oxoglutarate | pH 8.5, 50°C, K35A mutant enzyme | Bacillus sp. (in: Bacteria) |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.6.1.21 | Bacillus sp. (in: Bacteria) | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.6.1.21 | recombinant wild-type and mutant enzymes, Resource Q, phenyl-superose | Bacillus sp. (in: Bacteria) |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.6.1.21 | D-alanine + 2-oxoisovalerate | - |
Bacillus sp. (in: Bacteria) | pyruvate + D-valine | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.6.1.21 | 0.5 | - |
D-alanine | pH 8.5, 50°C, Y31A mutant enzyme | Bacillus sp. (in: Bacteria) | |
2.6.1.21 | 1.3 | - |
D-alanine | pH 8.5, 50°C, E32A mutant enzyme | Bacillus sp. (in: Bacteria) | |
2.6.1.21 | 13.4 | - |
D-alanine | pH 8.5, 50°C, E32Q mutant enzyme | Bacillus sp. (in: Bacteria) | |
2.6.1.21 | 84.3 | - |
D-alanine | pH 8.5, 50°C, K35A mutant enzyme | Bacillus sp. (in: Bacteria) | |
2.6.1.21 | 85.6 | - |
D-alanine | pH 8.5, 50°C, E32D mutant enzyme | Bacillus sp. (in: Bacteria) | |
2.6.1.21 | 131 | - |
D-alanine | pH 8.5, 50°C, wild-type enzyme | Bacillus sp. (in: Bacteria) | |
2.6.1.21 | 145 | - |
D-alanine | pH 8.5, 50°C, V33A mutant enzyme | Bacillus sp. (in: Bacteria) |