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Literature summary extracted from

  • Ward, D.E.; Kengen, S.W.; van Der Oost, J.; de Vos, W.M.
    Purification and characterization of the alanine aminotransferase from the hyperthermophilic Archaeon pyrococcus furiosus and its role in alanine production (2000), J. Bacteriol., 182, 2559-2566.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.2 expression in Escherichia coli Pyrococcus furiosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.2 0.9
-
 2-oxoglutarate pH 7.2, 80°C, native enzyme Pyrococcus furiosus
2.6.1.2 1.1
-
 2-oxoglutarate pH 7.2, 80°C, recombinant enzyme Pyrococcus furiosus
2.6.1.2 2.8
-
 alanine pH 7.2, 80°C, native enzyme Pyrococcus furiosus
2.6.1.2 3.2
-
 L-alanine pH 7.2, 80°C, recombinant enzyme Pyrococcus furiosus
2.6.1.2 4.3
-
 glutamate pH 7.2, 80°C, native enzyme Pyrococcus furiosus
2.6.1.2 4.7
-
 glutamate pH 7.2, 80°C, recombinant enzyme Pyrococcus furiosus
2.6.1.2 5.4
-
 pyruvate pH 7.2, 80°C, native enzyme Pyrococcus furiosus
2.6.1.2 5.6
-
 pyruvate pH 7.2, 80°C, recombinant enzyme Pyrococcus furiosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.2 46000
-
 2 * 46000, SDS-PAGE Pyrococcus furiosus
2.6.1.2 93400
-
 gel filtration Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.2 Pyrococcus furiosus Q9P9M8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.2 native enzyme, Q-Sepharose, phenyl-Sepharose, hydroxyapatite, S-Sepharose, Mono Q, Superdex 200, recombinant enzyme, heat treatment, Mono Q Pyrococcus furiosus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.2 158
-
 native enzyme Pyrococcus furiosus
2.6.1.2 243
-
 recombinant enzyme Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.2 aspartate + 2-oxoglutarate 14% of activity with glutamate and pyruvate Pyrococcus furiosus malate + L-glutamate
-
 ?
2.6.1.2 aspartate + pyruvate 10% of activity with alanine and 2-oxoglutarate Pyrococcus furiosus malate + alanine
-
 ?
2.6.1.2 L-alanine + 2-oxoglutarate
-
 Pyrococcus furiosus pyruvate + L-glutamate
-
 r

Subunits

EC Number Subunits Comment Organism
2.6.1.2 dimer 2 * 46000, SDS-PAGE Pyrococcus furiosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.2 additional information
-
 above 95°C, native and recombinant enzyme Pyrococcus furiosus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.6.1.2 30 95 native and recombinant enzyme Pyrococcus furiosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.2 6.5 7.8 native and recombinant enzyme Pyrococcus furiosus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.6.1.2 5.5 8.2 native and recombinant enzyme Pyrococcus furiosus