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Literature summary extracted from

  • Sanchez-Ferrer, A.; Bru, R.; Garcia-Carmona, F.
    Kinetic properties of polyphenoloxidase in organic solvents. A study in Brij 96-cyclohexane reverse micelles (1988), FEBS Lett., 233, 363-366.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.18.1 4 5 4-methyl catechol in poly-(10)-oxyethylene oleyl ether-cyclohexane reverse micelles Vitis vinifera
1.14.18.1 9
-
 4-methyl catechol
-
 Vitis vinifera

Organism

EC Number Organism UniProt Comment Textmining
1.14.18.1 Vitis vinifera
-
 monastrell grape
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.18.1 4-methylcatechol + O2
-
 Vitis vinifera 4-methyl-o-benzoquinone + H2O
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.18.1 25 40 catecholase activity Vitis vinifera

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.14.18.1 20 45 20°C, 85% of maximal activity, broad optimum between 25°C and 40°C, no activity above 45°C Vitis vinifera

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.18.1 5 6.5 catecholase activity entrapped in reverse micelles Vitis vinifera

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.14.18.1 4 8 pH 4.0: about 65% of maximal activity, pH 8.0: about 75% of maximal activity, catecholase activity Vitis vinifera