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Literature summary extracted from
- Characteristics of tyrosinase in AOT-isooctane reverse micelles (1989), Biotechnol. Bioeng., 34, 304-308.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.18.1 | 4-methylcatechol | - |
Mushroom |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.18.1 | 0.17 | - |
4-methylcatechol | - |
Mushroom | |
| 1.14.18.1 | 0.17 | - |
4-methylcatechol | in H2O | Mushroom | |
| 1.14.18.1 | 0.2 | - |
4-methylcatechol | measured in bis(2-ethylhexyl)sodium sulfosuccinate-isooctane reverse micelles | Mushroom | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.18.1 | Mushroom | - |
obtained from Sigma | - |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.18.1 | commercial preparation | - |
Mushroom | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.18.1 | 4-methylcatechol + O2 | - |
Mushroom | 4-methyl-o-benzoquinone + H2O | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.18.1 | 30 | - |
in bis(2-ethylhexyl)sodium sulfosuccinate-isooctane reverse micelles | Mushroom |
| 1.14.18.1 | 45 | - |
in H2O | Mushroom |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.18.1 | 6.2 | - |
free enzyme | Mushroom |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.18.1 | 4.5 | 7.5 | pH 4.5: about 45% of maximal activity, pH 7.5: about 60% of maximal activity, free enzyme | Mushroom |
| 1.14.18.1 | 5 | 9 | pH 5.0: about 30% of maximal activity, pH 9.0: about 50% of maximal activity, enzyme in reversed micelles | Mushroom |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.18.1 | 0.23 | - |
4-methylcatechol | in bis(2-ethylhexyl)sodium sulfosuccinate-isooctane reverse micelles | Mushroom | |
| 1.14.18.1 | 3.6 | - |
4-methylcatechol | in H2O, substrate inhibition | Mushroom | |
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Release 2023.1 (January 2023)
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