EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.14.18.1 | 1-Phenyl-2-thiourea | 1 mM, 94% inhibition | Aspergillus nidulans | |
1.14.18.1 | 8-hydroxyquinoline | 3 mM, 44% inhibition | Aspergillus nidulans | |
1.14.18.1 | azide | no inhibition by sodium azide | Aspergillus nidulans | |
1.14.18.1 | diethyldithiocarbamate | 0.1 mM, complete inhibition | Aspergillus nidulans | |
1.14.18.1 | Metabisulfite | 0.1 mM, 99% inhibition | Aspergillus nidulans | |
1.14.18.1 | o-phenanthroline hydrate | 3 mM, 30% inhibition | Aspergillus nidulans | |
1.14.18.1 | phenylhydrazine | 1 mM, 86% inhibition, noncompetitive inhibition | Aspergillus nidulans |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.18.1 | 2 | 14 | Hydroquinone monomethylether | range of estimated values for crude or purified extracts | Aspergillus nidulans |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.14.18.1 | extracellular | - |
Aspergillus nidulans | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.14.18.1 | copper | 2.1 copper atoms per polypeptide, enzyme form B | Aspergillus nidulans | |
1.14.18.1 | Zn2+ | 0.9 mol per polypeptide | Aspergillus nidulans |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.18.1 | 48000 | - |
x * 48000, enzyme form B, SDS-PAGE | Aspergillus nidulans |
1.14.18.1 | 50000 | - |
x * 50000, enzyme form A, SDS-PAGE | Aspergillus nidulans |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.18.1 | N-acetyl-6-hydroxytryptophan + O2 | Aspergillus nidulans | - |
? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.18.1 | Aspergillus nidulans | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.18.1 | DEAE-Sepharose, Phenyl Sepharose, enzyme form A and B | Aspergillus nidulans |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.14.18.1 | conidiophore | - |
Aspergillus nidulans | - |
1.14.18.1 | culture filtrate | - |
Aspergillus nidulans | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.14.18.1 | additional information | - |
enzyme form A, 480.0 DELTA absorbance 470 nm/min/mg, enzyme form B, 258.6 DELTA absorbance 470 nm/min/mg | Aspergillus nidulans |
EC Number | Storage Stability | Organism |
---|---|---|
1.14.18.1 | 4°C, 3 weeks, no loss of activity | Aspergillus nidulans |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.18.1 | 4-methylcatechol + O2 | - |
Aspergillus nidulans | 4-methyl-o-benzoquinone + H2O | - |
? | |
1.14.18.1 | catechol + 1/2 O2 | - |
Aspergillus nidulans | 1,2-benzoquinone + H2O | - |
? | |
1.14.18.1 | chlorogenic acid + O2 | - |
Aspergillus nidulans | chlorogenoquinone + H2O | - |
? | |
1.14.18.1 | D-catechin + O2 | - |
Aspergillus nidulans | ? | - |
? | |
1.14.18.1 | hydroquinone monomethylether + O2 | - |
Aspergillus nidulans | quinone monomethylether | - |
? | |
1.14.18.1 | N-acetyl-6-hydroxytryptophan + O2 | - |
Aspergillus nidulans | ? | - |
? | |
1.14.18.1 | o-methoxyphenol + O2 + AH2 | trivial name guaiacol | Aspergillus nidulans | 1,2-dihydroxy-3-methoxybenzene + H2O + A | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.14.18.1 | ? | x * 48000, enzyme form B, SDS-PAGE | Aspergillus nidulans |
1.14.18.1 | ? | x * 50000, enzyme form A, SDS-PAGE | Aspergillus nidulans |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.18.1 | 7 | - |
with hydroquinone monomethylether | Aspergillus nidulans |