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Literature summary extracted from

  • Yagi, K.; Nishimi, M.
    Expression of recombinant L-gulono-gamma-lactone oxidase (1997), Methods Enzymol., 279, 24-29.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.3.8 expressed in COS1-cells Rattus norvegicus
1.1.3.8 expressed in silkworm cell line BmN4 with baculovirus vector Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.8 51000
-
 expressed in silkworm cells, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.8 L-gulono-1,4-lactone + O2 Rattus norvegicus
-
 L-xylo-hex-3-ulonolactone + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.8 no activity in Cavia porcellus
-
 guinea pig
-
1.1.3.8 no activity in Homo sapiens
-
-
-
1.1.3.8 no activity in primates
-
-
-
1.1.3.8 Rattus norvegicus
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.3.8 L-gulono-1,4-lactone + O2 = L-xylo-hex-2-ulono-1,4-lactone + H2O2 product isomerizes spontaneously to L-ascorbate Rattus norvegicus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.3.8 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.8 0.016 0.02 enzyme expressed in silkworm cells Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.8 L-gulono-1,4-lactone + O2
-
 Rattus norvegicus L-xylo-hex-3-ulonolactone + H2O2
-
 ?
1.1.3.8 L-gulono-1,4-lactone + O2
-
 Rattus norvegicus L-xylo-hex-3-ulonolactone + H2O2 product isomerizes spontaneously to L-ascorbate ?

Synonyms

EC Number Synonyms Comment Organism
1.1.3.8 GLO
-
 Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.8 FAD covalently-bound Rattus norvegicus