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Literature summary extracted from

  • Lindl, T.; Kreuzaler, F.; Hahlbrock, K.
    Synthesis of p-coumaryl coenzyme A with a partially purified p-coumarate:CoA ligase from cell suspension cultures of soybean (Glycine max) (1973), Biochim. Biophys. Acta, 302, 457-464.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.2.1.12 Mg2+ required in a molar ratio of 1:1 with ATP Glycine max

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.2.1.12 55000
-
 gel filtration Glycine max

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.12 Glycine max
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.12 partial Glycine max

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.2.1.12 cell culture
-
 Glycine max
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.2.1.12 additional information
-
-
 Glycine max

Storage Stability

EC Number Storage Stability Organism
6.2.1.12 -20°C, several weeks without significant loss of activity Glycine max

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.12 ATP + 4-coumarate + CoA
-
 Glycine max AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
6.2.1.12 ATP + 4-methoxycinnamate + CoA 12% of the activity relative to 4-coumarate Glycine max AMP + diphosphate + 4-methoxycinnamoyl-CoA
-
 ?
6.2.1.12 ATP + caffeate + CoA 14% of the activity relative to 4-coumarate Glycine max AMP + diphosphate + caffeoyl-CoA
-
 ?
6.2.1.12 ATP + cinnamate + CoA 10% of the activity relative to 4-coumarate Glycine max AMP + diphosphate + cinnamoyl-CoA
-
 ?
6.2.1.12 ATP + ferulate + CoA 49% of the activity relative to 4-coumarate Glycine max AMP + diphosphate + feruloyl-CoA
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.2.1.12 30
-
-
 Glycine max

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
6.2.1.12 20 40 20°C: about 70% of maximal activity, 40°C: about 80% of maximal activity Glycine max

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2.1.12 8.5
-
-
 Glycine max

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.2.1.12 7.2 9.5 about 55% of maximal activity at pH 7.2 and 9.5 Glycine max